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Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus)

Variations in water salinity and other extrinsic factors have been shown to induce changes in feeding rhythms and growth in fish. However, it is unknown whether appetite-related hormones mediate these changes in Nile tilapia (Oreochromis niloticus), an important species for aquaculture in several co...

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Autores principales: Martins, Amanda W. S., Dellagostin, Eduardo N., Blödorn, Eduardo B., Silveira, Tony Leandro R., Sampaio, Luis A., Komninou, Eliza R., Varela Junior, Antonio S., Corcini, Carine D., Nunes, Leandro S., Remião, Mariana H., Collares, Gilberto L., Domingues, William B., Campos, Vinicius F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493302/
https://www.ncbi.nlm.nih.gov/pubmed/36160013
http://dx.doi.org/10.3389/fgene.2022.948228
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author Martins, Amanda W. S.
Dellagostin, Eduardo N.
Blödorn, Eduardo B.
Silveira, Tony Leandro R.
Sampaio, Luis A.
Komninou, Eliza R.
Varela Junior, Antonio S.
Corcini, Carine D.
Nunes, Leandro S.
Remião, Mariana H.
Collares, Gilberto L.
Domingues, William B.
Campos, Vinicius F.
author_facet Martins, Amanda W. S.
Dellagostin, Eduardo N.
Blödorn, Eduardo B.
Silveira, Tony Leandro R.
Sampaio, Luis A.
Komninou, Eliza R.
Varela Junior, Antonio S.
Corcini, Carine D.
Nunes, Leandro S.
Remião, Mariana H.
Collares, Gilberto L.
Domingues, William B.
Campos, Vinicius F.
author_sort Martins, Amanda W. S.
collection PubMed
description Variations in water salinity and other extrinsic factors have been shown to induce changes in feeding rhythms and growth in fish. However, it is unknown whether appetite-related hormones mediate these changes in Nile tilapia (Oreochromis niloticus), an important species for aquaculture in several countries. This study aimed to evaluate the expression of genes responsible for appetite regulation and genes related to metabolic and physiological changes in tilapia exposed to different salinities. Moreover, the study proposed to sequence and to characterize the cart, cck, and pyy genes, and to quantify their expression in the brain and intestine of the fish by quantitative polymerase chain reaction (qPCR). The animals were exposed to three salinities: 0, 6, and 12 parts per thousand (ppt) of salt for 21 days. Furthermore, lipid peroxidation, reactive oxygen species, DNA damage, and membrane fluidity in blood cells were quantified by flow cytometry. The results indicated an increased expression of cart, pyy, and cck and a decreased expression of npy in the brain, and the same with cck and npy in the intestine of fish treated with 12 ppt. This modulation and other adaptive responses may have contributed to the decrease in weight gain, specific growth rate, and final weight. In addition, we showed oxidative damage in blood cells resulting from increasing salinity. These results provide essential data on O. niloticus when exposed to high salinities that have never been described before and generate knowledge necessary for developing biotechnologies that may help improve the production of economically important farmed fish.
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spelling pubmed-94933022022-09-23 Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus) Martins, Amanda W. S. Dellagostin, Eduardo N. Blödorn, Eduardo B. Silveira, Tony Leandro R. Sampaio, Luis A. Komninou, Eliza R. Varela Junior, Antonio S. Corcini, Carine D. Nunes, Leandro S. Remião, Mariana H. Collares, Gilberto L. Domingues, William B. Campos, Vinicius F. Front Genet Genetics Variations in water salinity and other extrinsic factors have been shown to induce changes in feeding rhythms and growth in fish. However, it is unknown whether appetite-related hormones mediate these changes in Nile tilapia (Oreochromis niloticus), an important species for aquaculture in several countries. This study aimed to evaluate the expression of genes responsible for appetite regulation and genes related to metabolic and physiological changes in tilapia exposed to different salinities. Moreover, the study proposed to sequence and to characterize the cart, cck, and pyy genes, and to quantify their expression in the brain and intestine of the fish by quantitative polymerase chain reaction (qPCR). The animals were exposed to three salinities: 0, 6, and 12 parts per thousand (ppt) of salt for 21 days. Furthermore, lipid peroxidation, reactive oxygen species, DNA damage, and membrane fluidity in blood cells were quantified by flow cytometry. The results indicated an increased expression of cart, pyy, and cck and a decreased expression of npy in the brain, and the same with cck and npy in the intestine of fish treated with 12 ppt. This modulation and other adaptive responses may have contributed to the decrease in weight gain, specific growth rate, and final weight. In addition, we showed oxidative damage in blood cells resulting from increasing salinity. These results provide essential data on O. niloticus when exposed to high salinities that have never been described before and generate knowledge necessary for developing biotechnologies that may help improve the production of economically important farmed fish. Frontiers Media S.A. 2022-09-08 /pmc/articles/PMC9493302/ /pubmed/36160013 http://dx.doi.org/10.3389/fgene.2022.948228 Text en Copyright © 2022 Martins, Dellagostin, Blödorn, Silveira, Sampaio, Komninou, Varela Junior, Corcini, Nunes, Remião, Collares, Domingues and Campos. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Martins, Amanda W. S.
Dellagostin, Eduardo N.
Blödorn, Eduardo B.
Silveira, Tony Leandro R.
Sampaio, Luis A.
Komninou, Eliza R.
Varela Junior, Antonio S.
Corcini, Carine D.
Nunes, Leandro S.
Remião, Mariana H.
Collares, Gilberto L.
Domingues, William B.
Campos, Vinicius F.
Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus)
title Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus)
title_full Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus)
title_fullStr Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus)
title_full_unstemmed Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus)
title_short Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus)
title_sort exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in nile tilapia (oreochromis niloticus)
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493302/
https://www.ncbi.nlm.nih.gov/pubmed/36160013
http://dx.doi.org/10.3389/fgene.2022.948228
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