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Discovery of Rickettsia spp. in mosquitoes collected in Georgia by metagenomics analysis and molecular characterization
Arthropods have a broad and expanding worldwide presence and can transmit a variety of viral, bacterial, and parasite pathogens. A number of Rickettsia and Orientia species associated with ticks, fleas, lice, and mites have been detected in, or isolated from, patients with febrile illness and/or ani...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493313/ https://www.ncbi.nlm.nih.gov/pubmed/36160204 http://dx.doi.org/10.3389/fmicb.2022.961090 |
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author | Pollio, Adam R. Jiang, Ju Lee, Sam S. Gandhi, Jaykumar S. Knott, Brian D. Chunashvili, Tamar Conte, Matthew A. Walls, Shannon D. Hulseberg, Christine E. Farris, Christina M. Reinbold-Wasson, Drew D. Hang, Jun |
author_facet | Pollio, Adam R. Jiang, Ju Lee, Sam S. Gandhi, Jaykumar S. Knott, Brian D. Chunashvili, Tamar Conte, Matthew A. Walls, Shannon D. Hulseberg, Christine E. Farris, Christina M. Reinbold-Wasson, Drew D. Hang, Jun |
author_sort | Pollio, Adam R. |
collection | PubMed |
description | Arthropods have a broad and expanding worldwide presence and can transmit a variety of viral, bacterial, and parasite pathogens. A number of Rickettsia and Orientia species associated with ticks, fleas, lice, and mites have been detected in, or isolated from, patients with febrile illness and/or animal reservoirs throughout the world. Mosquitoes are not currently considered vectors for Rickettsia spp. pathogens to humans or to animals. In this study, we conducted a random metagenome next-generation sequencing (NGS) of 475 pools of Aedes, Culex, and Culiseta species of mosquitoes collected in Georgia from 2018 to 2019, identifying rickettsial gene sequences in 33 pools of mosquitoes. We further confirmed the findings of the Rickettsia by genus-specific quantitative PCR (qPCR) and multi-locus sequence typing (MLST). The NGS and MLST results indicate that Rickettsia spp. are closely related to Rickettsia bellii, which is not known to be pathogenic in humans. The results, together with other reports of Rickettsia spp. in mosquitoes and the susceptibility and transmissibility experiments, suggest that mosquitoes may play a role in the transmission cycle of Rickettsia spp. |
format | Online Article Text |
id | pubmed-9493313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94933132022-09-23 Discovery of Rickettsia spp. in mosquitoes collected in Georgia by metagenomics analysis and molecular characterization Pollio, Adam R. Jiang, Ju Lee, Sam S. Gandhi, Jaykumar S. Knott, Brian D. Chunashvili, Tamar Conte, Matthew A. Walls, Shannon D. Hulseberg, Christine E. Farris, Christina M. Reinbold-Wasson, Drew D. Hang, Jun Front Microbiol Microbiology Arthropods have a broad and expanding worldwide presence and can transmit a variety of viral, bacterial, and parasite pathogens. A number of Rickettsia and Orientia species associated with ticks, fleas, lice, and mites have been detected in, or isolated from, patients with febrile illness and/or animal reservoirs throughout the world. Mosquitoes are not currently considered vectors for Rickettsia spp. pathogens to humans or to animals. In this study, we conducted a random metagenome next-generation sequencing (NGS) of 475 pools of Aedes, Culex, and Culiseta species of mosquitoes collected in Georgia from 2018 to 2019, identifying rickettsial gene sequences in 33 pools of mosquitoes. We further confirmed the findings of the Rickettsia by genus-specific quantitative PCR (qPCR) and multi-locus sequence typing (MLST). The NGS and MLST results indicate that Rickettsia spp. are closely related to Rickettsia bellii, which is not known to be pathogenic in humans. The results, together with other reports of Rickettsia spp. in mosquitoes and the susceptibility and transmissibility experiments, suggest that mosquitoes may play a role in the transmission cycle of Rickettsia spp. Frontiers Media S.A. 2022-09-08 /pmc/articles/PMC9493313/ /pubmed/36160204 http://dx.doi.org/10.3389/fmicb.2022.961090 Text en Copyright © 2022 Pollio, Jiang, Lee, Gandhi, Knott, Chunashvili, Conte, Walls, Hulseberg, Farris, Reinbold-Wasson and Hang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Pollio, Adam R. Jiang, Ju Lee, Sam S. Gandhi, Jaykumar S. Knott, Brian D. Chunashvili, Tamar Conte, Matthew A. Walls, Shannon D. Hulseberg, Christine E. Farris, Christina M. Reinbold-Wasson, Drew D. Hang, Jun Discovery of Rickettsia spp. in mosquitoes collected in Georgia by metagenomics analysis and molecular characterization |
title | Discovery of Rickettsia spp. in mosquitoes collected in Georgia by metagenomics analysis and molecular characterization |
title_full | Discovery of Rickettsia spp. in mosquitoes collected in Georgia by metagenomics analysis and molecular characterization |
title_fullStr | Discovery of Rickettsia spp. in mosquitoes collected in Georgia by metagenomics analysis and molecular characterization |
title_full_unstemmed | Discovery of Rickettsia spp. in mosquitoes collected in Georgia by metagenomics analysis and molecular characterization |
title_short | Discovery of Rickettsia spp. in mosquitoes collected in Georgia by metagenomics analysis and molecular characterization |
title_sort | discovery of rickettsia spp. in mosquitoes collected in georgia by metagenomics analysis and molecular characterization |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493313/ https://www.ncbi.nlm.nih.gov/pubmed/36160204 http://dx.doi.org/10.3389/fmicb.2022.961090 |
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