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A time-course transcriptome analysis of wax gourd fruit development reveals predominant genes regulating taste and nutrition

Wax gourd, which belongs to Cucurbitaceae, is an excellent plant resource with the concomitant function of both medicine and foodstuff. Its unique taste and rich nutrition are deeply accepted by consumers. However, the main flavor and nutrients are still unclear, which restricts the quality breeding...

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Autores principales: Xue, Shudan, Wan, Xiaotong, Lu, Sen, Zhong, Yujuan, Xie, Dasen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493329/
https://www.ncbi.nlm.nih.gov/pubmed/36161022
http://dx.doi.org/10.3389/fpls.2022.971274
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author Xue, Shudan
Wan, Xiaotong
Lu, Sen
Zhong, Yujuan
Xie, Dasen
author_facet Xue, Shudan
Wan, Xiaotong
Lu, Sen
Zhong, Yujuan
Xie, Dasen
author_sort Xue, Shudan
collection PubMed
description Wax gourd, which belongs to Cucurbitaceae, is an excellent plant resource with the concomitant function of both medicine and foodstuff. Its unique taste and rich nutrition are deeply accepted by consumers. However, the main flavor and nutrients are still unclear, which restricts the quality breeding process of wax gourd. Here, we discovered that monosaccharides, malic acid and citrulline affect the flavor and nutrition of wax gourd and clarified the dynamic accumulation process of these metabolites. To gain insights into the underlying predominant genes regulating accumulation of these metabolites, we performed a time-course transcriptome analysis using RNA-sequencing analysis and compared the expression of screened genes among twenty-four germplasms with different metabolites levels. In addition, the expression abundance among the homologous genes were also analyzed. Finally, a total of 8 genes related to sugar [AGA2 (Bhi03G001926), SUS (Bhi12G001032)], malic acid [MDH (Bhi12G001426, Bhi01G000427), PEPC (Bhi12G000721, Bhi09G002867), ME (Bhi01G002616)] and citrulline [ASS (Bhi02G000401)], respectively were determined. In summary, understanding the core genes influencing taste or nutrition will provide a theoretical basis for fruit quality improvement in wax gourd.
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spelling pubmed-94933292022-09-23 A time-course transcriptome analysis of wax gourd fruit development reveals predominant genes regulating taste and nutrition Xue, Shudan Wan, Xiaotong Lu, Sen Zhong, Yujuan Xie, Dasen Front Plant Sci Plant Science Wax gourd, which belongs to Cucurbitaceae, is an excellent plant resource with the concomitant function of both medicine and foodstuff. Its unique taste and rich nutrition are deeply accepted by consumers. However, the main flavor and nutrients are still unclear, which restricts the quality breeding process of wax gourd. Here, we discovered that monosaccharides, malic acid and citrulline affect the flavor and nutrition of wax gourd and clarified the dynamic accumulation process of these metabolites. To gain insights into the underlying predominant genes regulating accumulation of these metabolites, we performed a time-course transcriptome analysis using RNA-sequencing analysis and compared the expression of screened genes among twenty-four germplasms with different metabolites levels. In addition, the expression abundance among the homologous genes were also analyzed. Finally, a total of 8 genes related to sugar [AGA2 (Bhi03G001926), SUS (Bhi12G001032)], malic acid [MDH (Bhi12G001426, Bhi01G000427), PEPC (Bhi12G000721, Bhi09G002867), ME (Bhi01G002616)] and citrulline [ASS (Bhi02G000401)], respectively were determined. In summary, understanding the core genes influencing taste or nutrition will provide a theoretical basis for fruit quality improvement in wax gourd. Frontiers Media S.A. 2022-09-08 /pmc/articles/PMC9493329/ /pubmed/36161022 http://dx.doi.org/10.3389/fpls.2022.971274 Text en Copyright © 2022 Xue, Wan, Lu, Zhong and Xie. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Xue, Shudan
Wan, Xiaotong
Lu, Sen
Zhong, Yujuan
Xie, Dasen
A time-course transcriptome analysis of wax gourd fruit development reveals predominant genes regulating taste and nutrition
title A time-course transcriptome analysis of wax gourd fruit development reveals predominant genes regulating taste and nutrition
title_full A time-course transcriptome analysis of wax gourd fruit development reveals predominant genes regulating taste and nutrition
title_fullStr A time-course transcriptome analysis of wax gourd fruit development reveals predominant genes regulating taste and nutrition
title_full_unstemmed A time-course transcriptome analysis of wax gourd fruit development reveals predominant genes regulating taste and nutrition
title_short A time-course transcriptome analysis of wax gourd fruit development reveals predominant genes regulating taste and nutrition
title_sort time-course transcriptome analysis of wax gourd fruit development reveals predominant genes regulating taste and nutrition
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493329/
https://www.ncbi.nlm.nih.gov/pubmed/36161022
http://dx.doi.org/10.3389/fpls.2022.971274
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