Significance of m(6)A regulatory factor in gene expression and immune function of osteoarthritis

One of the most prevalent posttranscriptional modifications of eukaryotic mRNA is the RNA N6-methyladenosine (m(6)A) regulator, which plays a significant role in various illnesses. The involvement of m(6)A regulators in osteoarthritis (OA) is not fully known. By comparing nonosteoarthritic and osteoarthritic patients, 26 important m(6)A regulators were identified from the gene expression omnibus GSE48556 dataset. Seven candidate m(6)A regulators (IGFBP3, WTAP, IGFBP1, HNRNPC, RBM15B, YTHDC1, and METTL3) were screened using a random forest model to assess the likelihood of OA. A column line graph model founded on seven m(6)A modulator candidates was created. According to decision curve analysis, patients might profit from the column line graph model. Based on chosen relevant m(6)A modifiers, a consensus clustering approach was utilized to categorize OA into two m(6)A categories (group A and group B). To measure the m(6)A pattern, a principal component analysis technique was created to generate the m(6)A score for every sample. Cluster A patients exhibited more excellent m(6)A scores than cluster B patients. Furthermore, we discovered that patients with lower and higher m(6)A scores had varied immunological responses using the m(6)A type. At last, m(6)A regulators contribute significantly to the progression of OA. Our research on m(6)A patterns might help to guide further OA immunotherapeutic techniques.