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Physiological genetic variation in tomato fruit chilling tolerance during postharvest storage

Storage at low temperatures is a common practice to prolong postharvest life of fruit and vegetables with a minimal negative impact on human/environmental health. Storage at low temperatures, however, can be restricted due to produce susceptibility to non-freezing chilling temperatures, when injurie...

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Autores principales: David, Sivan, Levin, Elena, Fallik, Elazar, Alkalai-Tuvia, Sharon, Foolad, Majid R., Lers, Amnon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493348/
https://www.ncbi.nlm.nih.gov/pubmed/36160961
http://dx.doi.org/10.3389/fpls.2022.991983
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author David, Sivan
Levin, Elena
Fallik, Elazar
Alkalai-Tuvia, Sharon
Foolad, Majid R.
Lers, Amnon
author_facet David, Sivan
Levin, Elena
Fallik, Elazar
Alkalai-Tuvia, Sharon
Foolad, Majid R.
Lers, Amnon
author_sort David, Sivan
collection PubMed
description Storage at low temperatures is a common practice to prolong postharvest life of fruit and vegetables with a minimal negative impact on human/environmental health. Storage at low temperatures, however, can be restricted due to produce susceptibility to non-freezing chilling temperatures, when injuries such as physiological disorders and decays may result in unmarketable produce. We have investigated tomato fruit response to postharvest chilling stress in a recombinant inbred line (RIL) population developed from a cross between a chilling-sensitive cultivated tomato (Solanum lycopersicum L.) breeding line and a chilling-tolerant inbred accession of the tomato wild species S. pimpinellifolium L. Screening of the fruit of 148 RILs under cold storage (1.5°C) indicated presence of significant variations in chilling tolerance, manifested by varying degrees of fruit injury. Two extremely contrasting groups of RILs were identified, chilling-tolerant and chilling-sensitive RILs. The RILs in the two groups were further investigated under chilling stress conditions, and several physiological parameters, including weight loss, chlorophyll fluorescence parameters Fv/Fm, and Performance Index (PI), were determined to be efficient markers for identifying response to chilling stress in postharvest fruit. The Fv/Fm values reflected the physiological damages endured by the fruit after cold storage, and PI was a sensitive marker for early changes in photosystem II function. These two parameters were early indicators of chilling response before occurrence of visible chilling injuries. Antioxidant activities and ascorbic acid content were significantly higher in the chilling-tolerant than the chilling-sensitive lines. Further, the expression of C-repeat/DREB binding factors (CBFs) genes swiftly changed within 1-hr of fruit exposure to the chilling temperature, and the SlCBF1 transcript level was generally higher in the chilling-tolerant than chilling-sensitive lines after 2-hr exposure to the low temperature. This research demonstrates the presence of potential genetic variation in fruit chilling tolerance in the tomato RIL population. Further investigation of the RIL population is underway to better understand the genetic, physiological, and biochemical mechanisms involved in postharvest fruit chilling tolerance in tomato.
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spelling pubmed-94933482022-09-23 Physiological genetic variation in tomato fruit chilling tolerance during postharvest storage David, Sivan Levin, Elena Fallik, Elazar Alkalai-Tuvia, Sharon Foolad, Majid R. Lers, Amnon Front Plant Sci Plant Science Storage at low temperatures is a common practice to prolong postharvest life of fruit and vegetables with a minimal negative impact on human/environmental health. Storage at low temperatures, however, can be restricted due to produce susceptibility to non-freezing chilling temperatures, when injuries such as physiological disorders and decays may result in unmarketable produce. We have investigated tomato fruit response to postharvest chilling stress in a recombinant inbred line (RIL) population developed from a cross between a chilling-sensitive cultivated tomato (Solanum lycopersicum L.) breeding line and a chilling-tolerant inbred accession of the tomato wild species S. pimpinellifolium L. Screening of the fruit of 148 RILs under cold storage (1.5°C) indicated presence of significant variations in chilling tolerance, manifested by varying degrees of fruit injury. Two extremely contrasting groups of RILs were identified, chilling-tolerant and chilling-sensitive RILs. The RILs in the two groups were further investigated under chilling stress conditions, and several physiological parameters, including weight loss, chlorophyll fluorescence parameters Fv/Fm, and Performance Index (PI), were determined to be efficient markers for identifying response to chilling stress in postharvest fruit. The Fv/Fm values reflected the physiological damages endured by the fruit after cold storage, and PI was a sensitive marker for early changes in photosystem II function. These two parameters were early indicators of chilling response before occurrence of visible chilling injuries. Antioxidant activities and ascorbic acid content were significantly higher in the chilling-tolerant than the chilling-sensitive lines. Further, the expression of C-repeat/DREB binding factors (CBFs) genes swiftly changed within 1-hr of fruit exposure to the chilling temperature, and the SlCBF1 transcript level was generally higher in the chilling-tolerant than chilling-sensitive lines after 2-hr exposure to the low temperature. This research demonstrates the presence of potential genetic variation in fruit chilling tolerance in the tomato RIL population. Further investigation of the RIL population is underway to better understand the genetic, physiological, and biochemical mechanisms involved in postharvest fruit chilling tolerance in tomato. Frontiers Media S.A. 2022-09-08 /pmc/articles/PMC9493348/ /pubmed/36160961 http://dx.doi.org/10.3389/fpls.2022.991983 Text en Copyright © 2022 David, Levin, Fallik, Alkalai-Tuvia, Foolad and Lers. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
David, Sivan
Levin, Elena
Fallik, Elazar
Alkalai-Tuvia, Sharon
Foolad, Majid R.
Lers, Amnon
Physiological genetic variation in tomato fruit chilling tolerance during postharvest storage
title Physiological genetic variation in tomato fruit chilling tolerance during postharvest storage
title_full Physiological genetic variation in tomato fruit chilling tolerance during postharvest storage
title_fullStr Physiological genetic variation in tomato fruit chilling tolerance during postharvest storage
title_full_unstemmed Physiological genetic variation in tomato fruit chilling tolerance during postharvest storage
title_short Physiological genetic variation in tomato fruit chilling tolerance during postharvest storage
title_sort physiological genetic variation in tomato fruit chilling tolerance during postharvest storage
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493348/
https://www.ncbi.nlm.nih.gov/pubmed/36160961
http://dx.doi.org/10.3389/fpls.2022.991983
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