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Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant
Erythrina velutina is a Brazilian native tree of the Caatinga (a unique semiarid biome). It is widely used in traditional medicine showing anti-inflammatory and central nervous system modulating activities. The species is a rich source of specialized metabolites, mostly alkaloids and flavonoids. To...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493460/ https://www.ncbi.nlm.nih.gov/pubmed/36161018 http://dx.doi.org/10.3389/fpls.2022.947558 |
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author | Chacon, Daisy Sotero Santos, Marlon Dias Mariano Bonilauri, Bernardo Vilasboa, Johnatan da Costa, Cibele Tesser da Silva, Ivanice Bezerra Torres, Taffarel de Melo de Araújo, Thiago Ferreira Roque, Alan de Araújo Pilon, Alan Cesar Selegatto, Denise Medeiros Freire, Rafael Teixeira Reginaldo, Fernanda Priscila Santos Voigt, Eduardo Luiz Zuanazzi, José Angelo Silveira Scortecci, Kátia Castanho Cavalheiro, Alberto José Lopes, Norberto Peporine Ferreira, Leandro De Santis dos Santos, Leandro Vieira Fontes, Wagner de Sousa, Marcelo Valle Carvalho, Paulo Costa Fett-Neto, Arthur Germano Giordani, Raquel Brandt |
author_facet | Chacon, Daisy Sotero Santos, Marlon Dias Mariano Bonilauri, Bernardo Vilasboa, Johnatan da Costa, Cibele Tesser da Silva, Ivanice Bezerra Torres, Taffarel de Melo de Araújo, Thiago Ferreira Roque, Alan de Araújo Pilon, Alan Cesar Selegatto, Denise Medeiros Freire, Rafael Teixeira Reginaldo, Fernanda Priscila Santos Voigt, Eduardo Luiz Zuanazzi, José Angelo Silveira Scortecci, Kátia Castanho Cavalheiro, Alberto José Lopes, Norberto Peporine Ferreira, Leandro De Santis dos Santos, Leandro Vieira Fontes, Wagner de Sousa, Marcelo Valle Carvalho, Paulo Costa Fett-Neto, Arthur Germano Giordani, Raquel Brandt |
author_sort | Chacon, Daisy Sotero |
collection | PubMed |
description | Erythrina velutina is a Brazilian native tree of the Caatinga (a unique semiarid biome). It is widely used in traditional medicine showing anti-inflammatory and central nervous system modulating activities. The species is a rich source of specialized metabolites, mostly alkaloids and flavonoids. To date, genomic information, biosynthesis, and regulation of flavonoids remain unknown in this woody plant. As part of a larger ongoing research goal to better understand specialized metabolism in plants inhabiting the harsh conditions of the Caatinga, the present study focused on this important class of bioactive phenolics. Leaves and seeds of plants growing in their natural habitat had their metabolic and proteomic profiles analyzed and integrated with transcriptome data. As a result, 96 metabolites (including 43 flavonoids) were annotated. Transcripts of the flavonoid pathway totaled 27, of which EvCHI, EvCHR, EvCHS, EvCYP75A and EvCYP75B1 were identified as putative main targets for modulating the accumulation of these metabolites. The highest correspondence of mRNA vs. protein was observed in the differentially expressed transcripts. In addition, 394 candidate transcripts encoding for transcription factors distributed among the bHLH, ERF, and MYB families were annotated. Based on interaction network analyses, several putative genes of the flavonoid pathway and transcription factors were related, particularly TFs of the MYB family. Expression patterns of transcripts involved in flavonoid biosynthesis and those involved in responses to biotic and abiotic stresses were discussed in detail. Overall, these findings provide a base for the understanding of molecular and metabolic responses in this medicinally important species. Moreover, the identification of key regulatory targets for future studies aiming at bioactive metabolite production will be facilitated. |
format | Online Article Text |
id | pubmed-9493460 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94934602022-09-23 Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant Chacon, Daisy Sotero Santos, Marlon Dias Mariano Bonilauri, Bernardo Vilasboa, Johnatan da Costa, Cibele Tesser da Silva, Ivanice Bezerra Torres, Taffarel de Melo de Araújo, Thiago Ferreira Roque, Alan de Araújo Pilon, Alan Cesar Selegatto, Denise Medeiros Freire, Rafael Teixeira Reginaldo, Fernanda Priscila Santos Voigt, Eduardo Luiz Zuanazzi, José Angelo Silveira Scortecci, Kátia Castanho Cavalheiro, Alberto José Lopes, Norberto Peporine Ferreira, Leandro De Santis dos Santos, Leandro Vieira Fontes, Wagner de Sousa, Marcelo Valle Carvalho, Paulo Costa Fett-Neto, Arthur Germano Giordani, Raquel Brandt Front Plant Sci Plant Science Erythrina velutina is a Brazilian native tree of the Caatinga (a unique semiarid biome). It is widely used in traditional medicine showing anti-inflammatory and central nervous system modulating activities. The species is a rich source of specialized metabolites, mostly alkaloids and flavonoids. To date, genomic information, biosynthesis, and regulation of flavonoids remain unknown in this woody plant. As part of a larger ongoing research goal to better understand specialized metabolism in plants inhabiting the harsh conditions of the Caatinga, the present study focused on this important class of bioactive phenolics. Leaves and seeds of plants growing in their natural habitat had their metabolic and proteomic profiles analyzed and integrated with transcriptome data. As a result, 96 metabolites (including 43 flavonoids) were annotated. Transcripts of the flavonoid pathway totaled 27, of which EvCHI, EvCHR, EvCHS, EvCYP75A and EvCYP75B1 were identified as putative main targets for modulating the accumulation of these metabolites. The highest correspondence of mRNA vs. protein was observed in the differentially expressed transcripts. In addition, 394 candidate transcripts encoding for transcription factors distributed among the bHLH, ERF, and MYB families were annotated. Based on interaction network analyses, several putative genes of the flavonoid pathway and transcription factors were related, particularly TFs of the MYB family. Expression patterns of transcripts involved in flavonoid biosynthesis and those involved in responses to biotic and abiotic stresses were discussed in detail. Overall, these findings provide a base for the understanding of molecular and metabolic responses in this medicinally important species. Moreover, the identification of key regulatory targets for future studies aiming at bioactive metabolite production will be facilitated. Frontiers Media S.A. 2022-09-08 /pmc/articles/PMC9493460/ /pubmed/36161018 http://dx.doi.org/10.3389/fpls.2022.947558 Text en Copyright © 2022 Chacon, Santos, Bonilauri, Vilasboa, da Costa, da Silva, Torres, de Araújo, Roque, Pilon, Selegatto, Freire, Reginaldo, Voigt, Zuanazzi, Scortecci, Cavalheiro, Lopes, Ferreira, Santos, Fontes, Sousa, Carvalho, Fett-Neto and Giordani. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Chacon, Daisy Sotero Santos, Marlon Dias Mariano Bonilauri, Bernardo Vilasboa, Johnatan da Costa, Cibele Tesser da Silva, Ivanice Bezerra Torres, Taffarel de Melo de Araújo, Thiago Ferreira Roque, Alan de Araújo Pilon, Alan Cesar Selegatto, Denise Medeiros Freire, Rafael Teixeira Reginaldo, Fernanda Priscila Santos Voigt, Eduardo Luiz Zuanazzi, José Angelo Silveira Scortecci, Kátia Castanho Cavalheiro, Alberto José Lopes, Norberto Peporine Ferreira, Leandro De Santis dos Santos, Leandro Vieira Fontes, Wagner de Sousa, Marcelo Valle Carvalho, Paulo Costa Fett-Neto, Arthur Germano Giordani, Raquel Brandt Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant |
title | Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant |
title_full | Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant |
title_fullStr | Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant |
title_full_unstemmed | Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant |
title_short | Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant |
title_sort | non-target molecular network and putative genes of flavonoid biosynthesis in erythrina velutina willd., a brazilian semiarid native woody plant |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493460/ https://www.ncbi.nlm.nih.gov/pubmed/36161018 http://dx.doi.org/10.3389/fpls.2022.947558 |
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