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Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant

Erythrina velutina is a Brazilian native tree of the Caatinga (a unique semiarid biome). It is widely used in traditional medicine showing anti-inflammatory and central nervous system modulating activities. The species is a rich source of specialized metabolites, mostly alkaloids and flavonoids. To...

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Autores principales: Chacon, Daisy Sotero, Santos, Marlon Dias Mariano, Bonilauri, Bernardo, Vilasboa, Johnatan, da Costa, Cibele Tesser, da Silva, Ivanice Bezerra, Torres, Taffarel de Melo, de Araújo, Thiago Ferreira, Roque, Alan de Araújo, Pilon, Alan Cesar, Selegatto, Denise Medeiros, Freire, Rafael Teixeira, Reginaldo, Fernanda Priscila Santos, Voigt, Eduardo Luiz, Zuanazzi, José Angelo Silveira, Scortecci, Kátia Castanho, Cavalheiro, Alberto José, Lopes, Norberto Peporine, Ferreira, Leandro De Santis, dos Santos, Leandro Vieira, Fontes, Wagner, de Sousa, Marcelo Valle, Carvalho, Paulo Costa, Fett-Neto, Arthur Germano, Giordani, Raquel Brandt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493460/
https://www.ncbi.nlm.nih.gov/pubmed/36161018
http://dx.doi.org/10.3389/fpls.2022.947558
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author Chacon, Daisy Sotero
Santos, Marlon Dias Mariano
Bonilauri, Bernardo
Vilasboa, Johnatan
da Costa, Cibele Tesser
da Silva, Ivanice Bezerra
Torres, Taffarel de Melo
de Araújo, Thiago Ferreira
Roque, Alan de Araújo
Pilon, Alan Cesar
Selegatto, Denise Medeiros
Freire, Rafael Teixeira
Reginaldo, Fernanda Priscila Santos
Voigt, Eduardo Luiz
Zuanazzi, José Angelo Silveira
Scortecci, Kátia Castanho
Cavalheiro, Alberto José
Lopes, Norberto Peporine
Ferreira, Leandro De Santis
dos Santos, Leandro Vieira
Fontes, Wagner
de Sousa, Marcelo Valle
Carvalho, Paulo Costa
Fett-Neto, Arthur Germano
Giordani, Raquel Brandt
author_facet Chacon, Daisy Sotero
Santos, Marlon Dias Mariano
Bonilauri, Bernardo
Vilasboa, Johnatan
da Costa, Cibele Tesser
da Silva, Ivanice Bezerra
Torres, Taffarel de Melo
de Araújo, Thiago Ferreira
Roque, Alan de Araújo
Pilon, Alan Cesar
Selegatto, Denise Medeiros
Freire, Rafael Teixeira
Reginaldo, Fernanda Priscila Santos
Voigt, Eduardo Luiz
Zuanazzi, José Angelo Silveira
Scortecci, Kátia Castanho
Cavalheiro, Alberto José
Lopes, Norberto Peporine
Ferreira, Leandro De Santis
dos Santos, Leandro Vieira
Fontes, Wagner
de Sousa, Marcelo Valle
Carvalho, Paulo Costa
Fett-Neto, Arthur Germano
Giordani, Raquel Brandt
author_sort Chacon, Daisy Sotero
collection PubMed
description Erythrina velutina is a Brazilian native tree of the Caatinga (a unique semiarid biome). It is widely used in traditional medicine showing anti-inflammatory and central nervous system modulating activities. The species is a rich source of specialized metabolites, mostly alkaloids and flavonoids. To date, genomic information, biosynthesis, and regulation of flavonoids remain unknown in this woody plant. As part of a larger ongoing research goal to better understand specialized metabolism in plants inhabiting the harsh conditions of the Caatinga, the present study focused on this important class of bioactive phenolics. Leaves and seeds of plants growing in their natural habitat had their metabolic and proteomic profiles analyzed and integrated with transcriptome data. As a result, 96 metabolites (including 43 flavonoids) were annotated. Transcripts of the flavonoid pathway totaled 27, of which EvCHI, EvCHR, EvCHS, EvCYP75A and EvCYP75B1 were identified as putative main targets for modulating the accumulation of these metabolites. The highest correspondence of mRNA vs. protein was observed in the differentially expressed transcripts. In addition, 394 candidate transcripts encoding for transcription factors distributed among the bHLH, ERF, and MYB families were annotated. Based on interaction network analyses, several putative genes of the flavonoid pathway and transcription factors were related, particularly TFs of the MYB family. Expression patterns of transcripts involved in flavonoid biosynthesis and those involved in responses to biotic and abiotic stresses were discussed in detail. Overall, these findings provide a base for the understanding of molecular and metabolic responses in this medicinally important species. Moreover, the identification of key regulatory targets for future studies aiming at bioactive metabolite production will be facilitated.
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spelling pubmed-94934602022-09-23 Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant Chacon, Daisy Sotero Santos, Marlon Dias Mariano Bonilauri, Bernardo Vilasboa, Johnatan da Costa, Cibele Tesser da Silva, Ivanice Bezerra Torres, Taffarel de Melo de Araújo, Thiago Ferreira Roque, Alan de Araújo Pilon, Alan Cesar Selegatto, Denise Medeiros Freire, Rafael Teixeira Reginaldo, Fernanda Priscila Santos Voigt, Eduardo Luiz Zuanazzi, José Angelo Silveira Scortecci, Kátia Castanho Cavalheiro, Alberto José Lopes, Norberto Peporine Ferreira, Leandro De Santis dos Santos, Leandro Vieira Fontes, Wagner de Sousa, Marcelo Valle Carvalho, Paulo Costa Fett-Neto, Arthur Germano Giordani, Raquel Brandt Front Plant Sci Plant Science Erythrina velutina is a Brazilian native tree of the Caatinga (a unique semiarid biome). It is widely used in traditional medicine showing anti-inflammatory and central nervous system modulating activities. The species is a rich source of specialized metabolites, mostly alkaloids and flavonoids. To date, genomic information, biosynthesis, and regulation of flavonoids remain unknown in this woody plant. As part of a larger ongoing research goal to better understand specialized metabolism in plants inhabiting the harsh conditions of the Caatinga, the present study focused on this important class of bioactive phenolics. Leaves and seeds of plants growing in their natural habitat had their metabolic and proteomic profiles analyzed and integrated with transcriptome data. As a result, 96 metabolites (including 43 flavonoids) were annotated. Transcripts of the flavonoid pathway totaled 27, of which EvCHI, EvCHR, EvCHS, EvCYP75A and EvCYP75B1 were identified as putative main targets for modulating the accumulation of these metabolites. The highest correspondence of mRNA vs. protein was observed in the differentially expressed transcripts. In addition, 394 candidate transcripts encoding for transcription factors distributed among the bHLH, ERF, and MYB families were annotated. Based on interaction network analyses, several putative genes of the flavonoid pathway and transcription factors were related, particularly TFs of the MYB family. Expression patterns of transcripts involved in flavonoid biosynthesis and those involved in responses to biotic and abiotic stresses were discussed in detail. Overall, these findings provide a base for the understanding of molecular and metabolic responses in this medicinally important species. Moreover, the identification of key regulatory targets for future studies aiming at bioactive metabolite production will be facilitated. Frontiers Media S.A. 2022-09-08 /pmc/articles/PMC9493460/ /pubmed/36161018 http://dx.doi.org/10.3389/fpls.2022.947558 Text en Copyright © 2022 Chacon, Santos, Bonilauri, Vilasboa, da Costa, da Silva, Torres, de Araújo, Roque, Pilon, Selegatto, Freire, Reginaldo, Voigt, Zuanazzi, Scortecci, Cavalheiro, Lopes, Ferreira, Santos, Fontes, Sousa, Carvalho, Fett-Neto and Giordani. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Chacon, Daisy Sotero
Santos, Marlon Dias Mariano
Bonilauri, Bernardo
Vilasboa, Johnatan
da Costa, Cibele Tesser
da Silva, Ivanice Bezerra
Torres, Taffarel de Melo
de Araújo, Thiago Ferreira
Roque, Alan de Araújo
Pilon, Alan Cesar
Selegatto, Denise Medeiros
Freire, Rafael Teixeira
Reginaldo, Fernanda Priscila Santos
Voigt, Eduardo Luiz
Zuanazzi, José Angelo Silveira
Scortecci, Kátia Castanho
Cavalheiro, Alberto José
Lopes, Norberto Peporine
Ferreira, Leandro De Santis
dos Santos, Leandro Vieira
Fontes, Wagner
de Sousa, Marcelo Valle
Carvalho, Paulo Costa
Fett-Neto, Arthur Germano
Giordani, Raquel Brandt
Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant
title Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant
title_full Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant
title_fullStr Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant
title_full_unstemmed Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant
title_short Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant
title_sort non-target molecular network and putative genes of flavonoid biosynthesis in erythrina velutina willd., a brazilian semiarid native woody plant
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493460/
https://www.ncbi.nlm.nih.gov/pubmed/36161018
http://dx.doi.org/10.3389/fpls.2022.947558
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