Protocol to drive human monocyte-to-macrophage polarization in vitro using tumor conditioned media

Tumor-associated macrophages (TAMs) are key contributors to antitumor immunity. Here, we present a protocol to drive human monocyte-macrophage differentiation using tumor-derived conditioned media, followed by phenotypic and functional characterization of TAMs in vitro. We describe CD14+ cell isolat...

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Detalles Bibliográficos
Autores principales: George, Samantha, Georgouli, Mirella, Sanz-Moreno, Victoria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9494234/
https://www.ncbi.nlm.nih.gov/pubmed/36125932
http://dx.doi.org/10.1016/j.xpro.2022.101666

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9494234/
https://www.ncbi.nlm.nih.gov/pubmed/36125932
http://dx.doi.org/10.1016/j.xpro.2022.101666

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