Cargando…

Protocol to drive human monocyte-to-macrophage polarization in vitro using tumor conditioned media

Tumor-associated macrophages (TAMs) are key contributors to antitumor immunity. Here, we present a protocol to drive human monocyte-macrophage differentiation using tumor-derived conditioned media, followed by phenotypic and functional characterization of TAMs in vitro. We describe CD14+ cell isolat...

Descripción completa

Detalles Bibliográficos
Autores principales:	George, Samantha, Georgouli, Mirella, Sanz-Moreno, Victoria
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9494234/ https://www.ncbi.nlm.nih.gov/pubmed/36125932 http://dx.doi.org/10.1016/j.xpro.2022.101666

Ejemplares similares

Recent advances in tissue imaging for cancer research
por: Maiques, Oscar, et al.
Publicado: (2019)

TGFΒ-induced transcription in cancer
por: Cantelli, Gaia, et al.
Publicado: (2017)

In vitro protocol for the optimal induction of inflammation in human monocyte cell lines
por: Koganti, Raghuram, et al.
Publicado: (2023)

Generation of monocyte-derived tumor-associated macrophages using tumor-conditioned media provides a novel method to study tumor-associated macrophages in vitro
por: Benner, Brooke, et al.
Publicado: (2019)

Protocol for Evaluating Neuronal Polarity in Murine Models
por: Wilson, Carlos, et al.
Publicado: (2020)

Protocol for detecting macrophage-mediated cancer cell phagocytosis in vitro and in vivo
por: Xu, Chuan, et al.
Publicado: (2022)

Protocol for simulating macrophage signal transduction and phenotype polarization using a large-scale mechanistic computational model
por: Zhao, Chen, et al.
Publicado: (2021)

Protocol for rapid obtention and fractionation of anaerobic bacterial conditioned media to study calcium signaling in enteroendocrine cells
por: Amorim Neto, Dionisio Pedro, et al.
Publicado: (2022)

In vitro induction of trained immunity in adherent human monocytes
por: Domínguez-Andrés, Jorge, et al.
Publicado: (2021)

Protocol for in vitro assessment of human monocyte transendothelial migration using a high-throughput live cell imaging system
por: Ladaigue, Ségolène, et al.
Publicado: (2023)

Intratumoral Monocyte Transfer to Examine Monocyte Differentiation in the Tumor Microenvironment
por: Devalaraja, Samir, et al.
Publicado: (2020)

MSCs’ conditioned media cytokine and growth factor profiles and their impact on macrophage polarization
por: Peshkova, Maria, et al.
Publicado: (2023)

Ontogeny and Polarization of Macrophages in Inflammation: Blood Monocytes Versus Tissue Macrophages
por: Dey, Adwitia, et al.
Publicado: (2015)

Characterization of polarization states of canine monocyte derived macrophages
por: Lyu, Qingkang, et al.
Publicado: (2023)

Lipopolysaccharide Tolerance in Human Primary Monocytes and Polarized Macrophages
por: Li, Hui, et al.
Publicado: (2023)

Characteristics, prognostic determinants of monocytes, macrophages and T cells in acute coronary syndrome: protocol for a multicenter, prospective cohort study
por: Lin, Muzhi, et al.
Publicado: (2023)

Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions
por: Giacomello, Ginevra, et al.
Publicado: (2022)

Generation and Functional Characterization of Monocytes and Macrophages Derived from Human Induced Pluripotent Stem Cells
por: Cao, Xu, et al.
Publicado: (2020)

Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro
por: Schoppmeyer, Rouven, et al.
Publicado: (2022)

Effect of Technology and Digital Media Use on Adolescent Health and Development: Protocol for a Multimethod Longitudinal Study
por: Cascio, Christopher N, et al.
Publicado: (2023)

Preparation of human primary macrophages to study the polarization from monocyte-derived macrophages to pro- or anti-inflammatory macrophages at biomaterial interface in vitro
por: Parisi, Ludovica, et al.
Publicado: (2023)

Characterization of macrophage infiltration and polarization by double fluorescence immunostaining in mouse colonic mucosa
por: Chiloeches, María López, et al.
Publicado: (2021)

In vitro flow cytometry assay to assess primary human and mouse macrophage phagocytosis of live cells
por: Liu, Samantha Y., et al.
Publicado: (2023)

Medium throughput protocol for genome-based quantification of intracellular mycobacterial loads and macrophage survival during in vitro infection
por: Yabaji, Shivraj M., et al.
Publicado: (2022)

Specific function and modulation of teleost monocytes/macrophages: polarization and phagocytosis
por: Lu, Xin-Jiang, et al.
Publicado: (2019)

The generation, activation, and polarization of monocyte-derived macrophages in human malignancies
por: Chaintreuil, Paul, et al.
Publicado: (2023)

Heterogeneity of Phenotypic and Functional Changes to Porcine Monocyte-Derived Macrophages Triggered by Diverse Polarizing Factors In Vitro
por: Franzoni, Giulia, et al.
Publicado: (2023)

Assessing the physiological effect of non-driving-related task performance in conditionally automated driving systems: A systematic review and meta-analysis protocol
por: Coyne, Rory, et al.
Publicado: (2023)

In Vitro Generation of Monocyte-Derived Macrophages under Serum-Free Conditions Improves Their Tumor Promoting Functions
por: Rey-Giraud, Flora, et al.
Publicado: (2012)

MicroRNA-155 contributes to enhanced resistance to apoptosis in monocytes from patients with rheumatoid arthritis
por: Rajasekhar, Megha, et al.
Publicado: (2017)

Protocol for a systematic review of prognostic models for recurrent events in chronic conditions
por: Watson, Victoria, et al.
Publicado: (2020)

Transcriptomic Analysis of Macrophage Polarization Protocols: Vitamin D(3) or IL-4 and IL-13 Do Not Polarize THP-1 Monocytes into Reliable M2 Macrophages
por: Rynikova, Maria, et al.
Publicado: (2023)

The effects of feedback and incentive-based insurance on driving behaviours: study approach and protocols
por: Stevenson, Mark, et al.
Publicado: (2018)

In Vitro Screening Method for Characterization of Macrophage Activation Responses
por: Lewis, Brandon W., et al.
Publicado: (2022)

The effects of conditioned media generated by polarized macrophages on the cellular behaviours of bone marrow mesenchymal stem cells
por: He, Xiao‐Tao, et al.
Publicado: (2017)

Intestinal monocytes and macrophages are required for T cell polarization in response to Citrobacter rodentium
por: Schreiber, Heidi A., et al.
Publicado: (2013)

Altered monocyte differentiation and macrophage polarization patterns in patients with breast cancer
por: Hung, Chih-Hsing, et al.
Publicado: (2018)

Caspase Inhibition Modulates Monocyte-Derived Macrophage Polarization in Damaged Tissues
por: Solier, Stéphanie, et al.
Publicado: (2023)

Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human
por: Maiques, Oscar, et al.
Publicado: (2022)

Macrophages are exploited from an innate wound healing response to facilitate cancer metastasis
por: Muliaditan, Tamara, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_cetjopfmt45bd1pebmj5s62tku