Protocol for differential centrifugation-based separation and characterization of apoptotic vesicles derived from human mesenchymal stem cells

Apoptotic vesicles (apoVs) are specific extracellular vesicles generated during apoptosis and play important roles in multiple physiological and pathophysiological settings. Here, we present a protocol using differential centrifugation to separate apoVs from human mesenchymal stem cells (MSCs) after...

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Detalles Bibliográficos
Autores principales: Chen, Da, Zhao, Zixuan, Zhang, Kai, Jin, Fang, Zheng, Chenxi, Jin, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9494288/
https://www.ncbi.nlm.nih.gov/pubmed/36129822
http://dx.doi.org/10.1016/j.xpro.2022.101695
Descripción
Sumario:Apoptotic vesicles (apoVs) are specific extracellular vesicles generated during apoptosis and play important roles in multiple physiological and pathophysiological settings. Here, we present a protocol using differential centrifugation to separate apoVs from human mesenchymal stem cells (MSCs) after induction of apoptosis. We describe how to characterize apoV size and morphology by nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM), and determination of specific biomarker expression by immunoblotting. Our protocol will be useful for preparing apoVs for further functional analysis. For complete details on the use and execution of this protocol, please refer to Zheng et al. (2021).

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