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Geobacter sulfurreducens metabolism at different donor/acceptor ratios

Geobacter species have great application potential in remediation processes and electrobiotechnology. In all applications, understanding the metabolism will enable target‐oriented optimization of the processes. The typical electron donor and carbon source of the Geobacter species is acetate, while f...

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Autores principales: Frühauf‐Wyllie, Hanna Marianne, Holtmann, Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9494351/
https://www.ncbi.nlm.nih.gov/pubmed/36314758
http://dx.doi.org/10.1002/mbo3.1322
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author Frühauf‐Wyllie, Hanna Marianne
Holtmann, Dirk
author_facet Frühauf‐Wyllie, Hanna Marianne
Holtmann, Dirk
author_sort Frühauf‐Wyllie, Hanna Marianne
collection PubMed
description Geobacter species have great application potential in remediation processes and electrobiotechnology. In all applications, understanding the metabolism will enable target‐oriented optimization of the processes. The typical electron donor and carbon source of the Geobacter species is acetate, while fumarate is the usual electron acceptor. Here, we could show that depending on the donor/acceptor ratio in batch cultivation of Geobacter sulfurreducens different product patterns occur. With a donor/acceptor ratio of 1:2.5 malate accumulated as an intermediate product but was metabolized to succinate subsequently. At the end of the cultivation, the ratio of fumarate consumed and succinate produced was approximately 1:1. When fumarate was added in excess, malate accumulated in the fermentation broth without further metabolization. After the addition of acetate to stationary cells, malate concentration decreased immediately and additional succinate was synthesized. Finally, it was shown that also resting cells of G. sulfurreducens could efficiently convert fumarate to malate without an additional electron donor. Overall, it was demonstrated that by altering the donor/acceptor ratio, targeted optimization of the metabolite conversion by G. sulfurreducens can be realized.
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spelling pubmed-94943512022-09-30 Geobacter sulfurreducens metabolism at different donor/acceptor ratios Frühauf‐Wyllie, Hanna Marianne Holtmann, Dirk Microbiologyopen Original Articles Geobacter species have great application potential in remediation processes and electrobiotechnology. In all applications, understanding the metabolism will enable target‐oriented optimization of the processes. The typical electron donor and carbon source of the Geobacter species is acetate, while fumarate is the usual electron acceptor. Here, we could show that depending on the donor/acceptor ratio in batch cultivation of Geobacter sulfurreducens different product patterns occur. With a donor/acceptor ratio of 1:2.5 malate accumulated as an intermediate product but was metabolized to succinate subsequently. At the end of the cultivation, the ratio of fumarate consumed and succinate produced was approximately 1:1. When fumarate was added in excess, malate accumulated in the fermentation broth without further metabolization. After the addition of acetate to stationary cells, malate concentration decreased immediately and additional succinate was synthesized. Finally, it was shown that also resting cells of G. sulfurreducens could efficiently convert fumarate to malate without an additional electron donor. Overall, it was demonstrated that by altering the donor/acceptor ratio, targeted optimization of the metabolite conversion by G. sulfurreducens can be realized. John Wiley and Sons Inc. 2022-09-22 /pmc/articles/PMC9494351/ /pubmed/36314758 http://dx.doi.org/10.1002/mbo3.1322 Text en © 2022 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Frühauf‐Wyllie, Hanna Marianne
Holtmann, Dirk
Geobacter sulfurreducens metabolism at different donor/acceptor ratios
title Geobacter sulfurreducens metabolism at different donor/acceptor ratios
title_full Geobacter sulfurreducens metabolism at different donor/acceptor ratios
title_fullStr Geobacter sulfurreducens metabolism at different donor/acceptor ratios
title_full_unstemmed Geobacter sulfurreducens metabolism at different donor/acceptor ratios
title_short Geobacter sulfurreducens metabolism at different donor/acceptor ratios
title_sort geobacter sulfurreducens metabolism at different donor/acceptor ratios
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9494351/
https://www.ncbi.nlm.nih.gov/pubmed/36314758
http://dx.doi.org/10.1002/mbo3.1322
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