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Iron metabolism mediates the relationship between Vitamin C and hepatic steatosis and fibrosis in NAFLD

Vitamin C (Vit C) and iron metabolism are closely related to metabolic disorders. However, the relation between iron storage protein ferritin and Vit C has not been elucidated. We aimed to investigate the crosstalk between Vit C and ferritin and its implications on non-alcoholic fatty liver disease...

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Autores principales: Hu, Zhengyu, Li, Yan, Ma, Bingwei, Lei, Saifei, Wang, Xingchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9494736/
https://www.ncbi.nlm.nih.gov/pubmed/36159474
http://dx.doi.org/10.3389/fnut.2022.952056
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author Hu, Zhengyu
Li, Yan
Ma, Bingwei
Lei, Saifei
Wang, Xingchun
author_facet Hu, Zhengyu
Li, Yan
Ma, Bingwei
Lei, Saifei
Wang, Xingchun
author_sort Hu, Zhengyu
collection PubMed
description Vitamin C (Vit C) and iron metabolism are closely related to metabolic disorders. However, the relation between iron storage protein ferritin and Vit C has not been elucidated. We aimed to investigate the crosstalk between Vit C and ferritin and its implications on non-alcoholic fatty liver disease (NAFLD). Clinical information of 3,614 subjects was obtained from the NHANES Public Data 2017–2018. FibroScan data, which estimates liver steatosis and fibrosis and Vit C, were selected to assess factors influencing NAFLD in this cross-sectional study. Ferritin and Vit C among different categories of liver steatosis and fibrosis were assessed by CAP and E value. Logistic regression and RCS models were used to analyze the correlations. In vitro study in hepG2 were conducted to validate the regulations. Ferritin increased while Vit C decreased with more severe hepatic steatosis and hepatic fibrosis (all P < 0.001). Logistic regression models indicated that increased serum ferritin was a risk factor for NAFLD while increased Vit C was a protective factor for NAFLD and hepatic fibrosis after adjusting the continuous and categorical variables. Vitamin C was negatively associated with ferritin. Further mediation analysis identified that ferritin mediates the impact of Vit C on NAFLD (P < 0.05) and cirrhosis (P < 0.001). The experiments on cellular level suggested Vit C alleviated PA/OA induced steatosis and maintains iron homeostasis through inhibiting PA/OA induced upregulation of iron bound protein ferritin and labile iron pool (LIP) induction in hepG2 cells. In conclusion, Vit C was a protective factor, whereas ferritin was a risk factor for hepatic steatosis and fibrosis. Vitamin C alleviated NAFLD and maintained iron homeostasis via ferritin suppression and LIP induction.
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spelling pubmed-94947362022-09-23 Iron metabolism mediates the relationship between Vitamin C and hepatic steatosis and fibrosis in NAFLD Hu, Zhengyu Li, Yan Ma, Bingwei Lei, Saifei Wang, Xingchun Front Nutr Nutrition Vitamin C (Vit C) and iron metabolism are closely related to metabolic disorders. However, the relation between iron storage protein ferritin and Vit C has not been elucidated. We aimed to investigate the crosstalk between Vit C and ferritin and its implications on non-alcoholic fatty liver disease (NAFLD). Clinical information of 3,614 subjects was obtained from the NHANES Public Data 2017–2018. FibroScan data, which estimates liver steatosis and fibrosis and Vit C, were selected to assess factors influencing NAFLD in this cross-sectional study. Ferritin and Vit C among different categories of liver steatosis and fibrosis were assessed by CAP and E value. Logistic regression and RCS models were used to analyze the correlations. In vitro study in hepG2 were conducted to validate the regulations. Ferritin increased while Vit C decreased with more severe hepatic steatosis and hepatic fibrosis (all P < 0.001). Logistic regression models indicated that increased serum ferritin was a risk factor for NAFLD while increased Vit C was a protective factor for NAFLD and hepatic fibrosis after adjusting the continuous and categorical variables. Vitamin C was negatively associated with ferritin. Further mediation analysis identified that ferritin mediates the impact of Vit C on NAFLD (P < 0.05) and cirrhosis (P < 0.001). The experiments on cellular level suggested Vit C alleviated PA/OA induced steatosis and maintains iron homeostasis through inhibiting PA/OA induced upregulation of iron bound protein ferritin and labile iron pool (LIP) induction in hepG2 cells. In conclusion, Vit C was a protective factor, whereas ferritin was a risk factor for hepatic steatosis and fibrosis. Vitamin C alleviated NAFLD and maintained iron homeostasis via ferritin suppression and LIP induction. Frontiers Media S.A. 2022-09-08 /pmc/articles/PMC9494736/ /pubmed/36159474 http://dx.doi.org/10.3389/fnut.2022.952056 Text en Copyright © 2022 Hu, Li, Ma, Lei and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Hu, Zhengyu
Li, Yan
Ma, Bingwei
Lei, Saifei
Wang, Xingchun
Iron metabolism mediates the relationship between Vitamin C and hepatic steatosis and fibrosis in NAFLD
title Iron metabolism mediates the relationship between Vitamin C and hepatic steatosis and fibrosis in NAFLD
title_full Iron metabolism mediates the relationship between Vitamin C and hepatic steatosis and fibrosis in NAFLD
title_fullStr Iron metabolism mediates the relationship between Vitamin C and hepatic steatosis and fibrosis in NAFLD
title_full_unstemmed Iron metabolism mediates the relationship between Vitamin C and hepatic steatosis and fibrosis in NAFLD
title_short Iron metabolism mediates the relationship between Vitamin C and hepatic steatosis and fibrosis in NAFLD
title_sort iron metabolism mediates the relationship between vitamin c and hepatic steatosis and fibrosis in nafld
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9494736/
https://www.ncbi.nlm.nih.gov/pubmed/36159474
http://dx.doi.org/10.3389/fnut.2022.952056
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