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CDH1 overexpression predicts bladder cancer from early stage and inversely correlates with immune infiltration
BACKGROUND: Bladder cancer (BC) seriously endangers public health, but effective biomarkers for BC diagnosis, particularly in the early stage, are still lacking. Identification of reliable biomarkers associated with early-stage BC is of great importance to early treatment and an improved outcome. ME...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9494810/ https://www.ncbi.nlm.nih.gov/pubmed/36131343 http://dx.doi.org/10.1186/s12894-022-01103-7 |
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author | Fan, Tao Xue, Liang Dong, Bingzheng He, Houguang Zhang, Wenda Hao, Lin Ma, Weiming Zang, Guanghui Han, Conghui Dong, Yang |
author_facet | Fan, Tao Xue, Liang Dong, Bingzheng He, Houguang Zhang, Wenda Hao, Lin Ma, Weiming Zang, Guanghui Han, Conghui Dong, Yang |
author_sort | Fan, Tao |
collection | PubMed |
description | BACKGROUND: Bladder cancer (BC) seriously endangers public health, but effective biomarkers for BC diagnosis, particularly in the early stage, are still lacking. Identification of reliable biomarkers associated with early-stage BC is of great importance to early treatment and an improved outcome. METHODS: Differentially expressed genes (DEGs) were identified using four publicly available early-stage BC gene-expression profiles. Protein–protein interaction (PPI) and survival analysis for hub genes was evaluated. The correlation between methylation of genes and prognosis was evaluated using the MethSurv database. Co-expressed genes were explored using Cancer Cell Line Encyclopedia database and the corresponding expression were assessed in vitro. The competing endogenous RNA network and the immune cell infiltration in BC were generated using data of The Cancer Genome Atlas. RESULTS: Ten hub genes of the 213 integrated DEGs were identified, including CDH1, IGFBP3, PPARG, SDC1, EPCAM, ACTA2, COL3A1, TPM1, ACTC1, and ACTN1. CDH1 appeared to increase from tumor initiation stage and negatively correlated with methylation. Six methylated sites in CDH1 indicated a good prognosis and one site indicated an aberrant prognosis. High CDH1 expression was negatively correlated with infiltrations by most immune cells, such as plasmacytoid dendritic cells (pDCs), regulatory T cells, macrophages, neutrophils, DCs, and natural killer cells. CDH1 was highly positively correlated with EPCAM and appeared to be directly regulated by miR-383. CONCLUSIONS: The identified oncogenic alterations provide theoretical support for the development of novel biomarkers to advance early-stage BC diagnosis and personalized therapy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12894-022-01103-7. |
format | Online Article Text |
id | pubmed-9494810 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-94948102022-09-23 CDH1 overexpression predicts bladder cancer from early stage and inversely correlates with immune infiltration Fan, Tao Xue, Liang Dong, Bingzheng He, Houguang Zhang, Wenda Hao, Lin Ma, Weiming Zang, Guanghui Han, Conghui Dong, Yang BMC Urol Research BACKGROUND: Bladder cancer (BC) seriously endangers public health, but effective biomarkers for BC diagnosis, particularly in the early stage, are still lacking. Identification of reliable biomarkers associated with early-stage BC is of great importance to early treatment and an improved outcome. METHODS: Differentially expressed genes (DEGs) were identified using four publicly available early-stage BC gene-expression profiles. Protein–protein interaction (PPI) and survival analysis for hub genes was evaluated. The correlation between methylation of genes and prognosis was evaluated using the MethSurv database. Co-expressed genes were explored using Cancer Cell Line Encyclopedia database and the corresponding expression were assessed in vitro. The competing endogenous RNA network and the immune cell infiltration in BC were generated using data of The Cancer Genome Atlas. RESULTS: Ten hub genes of the 213 integrated DEGs were identified, including CDH1, IGFBP3, PPARG, SDC1, EPCAM, ACTA2, COL3A1, TPM1, ACTC1, and ACTN1. CDH1 appeared to increase from tumor initiation stage and negatively correlated with methylation. Six methylated sites in CDH1 indicated a good prognosis and one site indicated an aberrant prognosis. High CDH1 expression was negatively correlated with infiltrations by most immune cells, such as plasmacytoid dendritic cells (pDCs), regulatory T cells, macrophages, neutrophils, DCs, and natural killer cells. CDH1 was highly positively correlated with EPCAM and appeared to be directly regulated by miR-383. CONCLUSIONS: The identified oncogenic alterations provide theoretical support for the development of novel biomarkers to advance early-stage BC diagnosis and personalized therapy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12894-022-01103-7. BioMed Central 2022-09-21 /pmc/articles/PMC9494810/ /pubmed/36131343 http://dx.doi.org/10.1186/s12894-022-01103-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Fan, Tao Xue, Liang Dong, Bingzheng He, Houguang Zhang, Wenda Hao, Lin Ma, Weiming Zang, Guanghui Han, Conghui Dong, Yang CDH1 overexpression predicts bladder cancer from early stage and inversely correlates with immune infiltration |
title | CDH1 overexpression predicts bladder cancer from early stage and inversely correlates with immune infiltration |
title_full | CDH1 overexpression predicts bladder cancer from early stage and inversely correlates with immune infiltration |
title_fullStr | CDH1 overexpression predicts bladder cancer from early stage and inversely correlates with immune infiltration |
title_full_unstemmed | CDH1 overexpression predicts bladder cancer from early stage and inversely correlates with immune infiltration |
title_short | CDH1 overexpression predicts bladder cancer from early stage and inversely correlates with immune infiltration |
title_sort | cdh1 overexpression predicts bladder cancer from early stage and inversely correlates with immune infiltration |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9494810/ https://www.ncbi.nlm.nih.gov/pubmed/36131343 http://dx.doi.org/10.1186/s12894-022-01103-7 |
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