Cargando…
Long noncoding RNA ZNFX1-AS1 promotes the invasion and proliferation of gastric cancer cells by regulating LIN28 and CAPR1N1
BACKGROUND: Long noncoding RNA (lncRNA) ZNFX1-AS1 (ZFAS1) is a newly discovered lncRNA, but its diagnostic value in gastric cancer is unclear. AIM: To investigate the potential role of ZFAS1 in gastric cancer and to evaluate the clinical significance of ZFAS1 as a biomarker for gastric cancer screen...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Baishideng Publishing Group Inc
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9494930/ https://www.ncbi.nlm.nih.gov/pubmed/36160641 http://dx.doi.org/10.3748/wjg.v28.i34.4973 |
_version_ | 1784793897570926592 |
---|---|
author | Zhuo, Zhong-Ling Xian, Hai-Peng Sun, Yu-Jing Long, Yan Liu, Chang Liang, Bin Zhao, Xiao-Tao |
author_facet | Zhuo, Zhong-Ling Xian, Hai-Peng Sun, Yu-Jing Long, Yan Liu, Chang Liang, Bin Zhao, Xiao-Tao |
author_sort | Zhuo, Zhong-Ling |
collection | PubMed |
description | BACKGROUND: Long noncoding RNA (lncRNA) ZNFX1-AS1 (ZFAS1) is a newly discovered lncRNA, but its diagnostic value in gastric cancer is unclear. AIM: To investigate the potential role of ZFAS1 in gastric cancer and to evaluate the clinical significance of ZFAS1 as a biomarker for gastric cancer screening. METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to screen for gastric cancer-associated lncRNAs in gastric cancer patients, gastric stromal tumor patients, gastritis or gastric ulcer patients, and healthy controls. Correlations between ZFAS1 expression and clinicopathological features were analyzed. The biological effects of ZFAS1 on the proliferation, migration, and invasion of gastric cancer cells were studied by MTT, colony formation, and transwell mi-gration assays. The potential mechanism of ZFAS1 was demonstrated using enzyme-linked immunosorbent assay and qRT-PCR. The relationship between ZFAS1 and tumorigenesis was demonstrated using in vivo tumor formation assays. RESULTS: The plasma level of lncRNA ZFAS1 was significantly higher in preoperative patients with gastric cancer than in individuals in the other 4 groups. Increased expression of ZFAS1 was significantly associated with lymph node metastasis, advanced TNM stage, and poor prognosis. ZFAS1 regulated the proliferation, migration, and invasion of gastric cancer cells and regulated the growth of gastric cancer cells in vivo. LIN28 and CAPRIN1 were identified as key downstream mediators of ZFAS1 in gastric cancer cells. CONCLUSION: LncRNA ZFAS1 promoted the invasion and proliferation of gastric cancer cells by modulating LIN28 and CAPRIN1 expression, suggesting that ZFAS1 can be used as a potential diagnostic and prognostic biomarker in gastric cancer. |
format | Online Article Text |
id | pubmed-9494930 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-94949302022-09-23 Long noncoding RNA ZNFX1-AS1 promotes the invasion and proliferation of gastric cancer cells by regulating LIN28 and CAPR1N1 Zhuo, Zhong-Ling Xian, Hai-Peng Sun, Yu-Jing Long, Yan Liu, Chang Liang, Bin Zhao, Xiao-Tao World J Gastroenterol Basic Study BACKGROUND: Long noncoding RNA (lncRNA) ZNFX1-AS1 (ZFAS1) is a newly discovered lncRNA, but its diagnostic value in gastric cancer is unclear. AIM: To investigate the potential role of ZFAS1 in gastric cancer and to evaluate the clinical significance of ZFAS1 as a biomarker for gastric cancer screening. METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to screen for gastric cancer-associated lncRNAs in gastric cancer patients, gastric stromal tumor patients, gastritis or gastric ulcer patients, and healthy controls. Correlations between ZFAS1 expression and clinicopathological features were analyzed. The biological effects of ZFAS1 on the proliferation, migration, and invasion of gastric cancer cells were studied by MTT, colony formation, and transwell mi-gration assays. The potential mechanism of ZFAS1 was demonstrated using enzyme-linked immunosorbent assay and qRT-PCR. The relationship between ZFAS1 and tumorigenesis was demonstrated using in vivo tumor formation assays. RESULTS: The plasma level of lncRNA ZFAS1 was significantly higher in preoperative patients with gastric cancer than in individuals in the other 4 groups. Increased expression of ZFAS1 was significantly associated with lymph node metastasis, advanced TNM stage, and poor prognosis. ZFAS1 regulated the proliferation, migration, and invasion of gastric cancer cells and regulated the growth of gastric cancer cells in vivo. LIN28 and CAPRIN1 were identified as key downstream mediators of ZFAS1 in gastric cancer cells. CONCLUSION: LncRNA ZFAS1 promoted the invasion and proliferation of gastric cancer cells by modulating LIN28 and CAPRIN1 expression, suggesting that ZFAS1 can be used as a potential diagnostic and prognostic biomarker in gastric cancer. Baishideng Publishing Group Inc 2022-09-14 2022-09-14 /pmc/articles/PMC9494930/ /pubmed/36160641 http://dx.doi.org/10.3748/wjg.v28.i34.4973 Text en ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved. https://creativecommons.org/licenses/by-nc/4.0/This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/ |
spellingShingle | Basic Study Zhuo, Zhong-Ling Xian, Hai-Peng Sun, Yu-Jing Long, Yan Liu, Chang Liang, Bin Zhao, Xiao-Tao Long noncoding RNA ZNFX1-AS1 promotes the invasion and proliferation of gastric cancer cells by regulating LIN28 and CAPR1N1 |
title | Long noncoding RNA ZNFX1-AS1 promotes the invasion and proliferation of gastric cancer cells by regulating LIN28 and CAPR1N1 |
title_full | Long noncoding RNA ZNFX1-AS1 promotes the invasion and proliferation of gastric cancer cells by regulating LIN28 and CAPR1N1 |
title_fullStr | Long noncoding RNA ZNFX1-AS1 promotes the invasion and proliferation of gastric cancer cells by regulating LIN28 and CAPR1N1 |
title_full_unstemmed | Long noncoding RNA ZNFX1-AS1 promotes the invasion and proliferation of gastric cancer cells by regulating LIN28 and CAPR1N1 |
title_short | Long noncoding RNA ZNFX1-AS1 promotes the invasion and proliferation of gastric cancer cells by regulating LIN28 and CAPR1N1 |
title_sort | long noncoding rna znfx1-as1 promotes the invasion and proliferation of gastric cancer cells by regulating lin28 and capr1n1 |
topic | Basic Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9494930/ https://www.ncbi.nlm.nih.gov/pubmed/36160641 http://dx.doi.org/10.3748/wjg.v28.i34.4973 |
work_keys_str_mv | AT zhuozhongling longnoncodingrnaznfx1as1promotestheinvasionandproliferationofgastriccancercellsbyregulatinglin28andcapr1n1 AT xianhaipeng longnoncodingrnaznfx1as1promotestheinvasionandproliferationofgastriccancercellsbyregulatinglin28andcapr1n1 AT sunyujing longnoncodingrnaznfx1as1promotestheinvasionandproliferationofgastriccancercellsbyregulatinglin28andcapr1n1 AT longyan longnoncodingrnaznfx1as1promotestheinvasionandproliferationofgastriccancercellsbyregulatinglin28andcapr1n1 AT liuchang longnoncodingrnaznfx1as1promotestheinvasionandproliferationofgastriccancercellsbyregulatinglin28andcapr1n1 AT liangbin longnoncodingrnaznfx1as1promotestheinvasionandproliferationofgastriccancercellsbyregulatinglin28andcapr1n1 AT zhaoxiaotao longnoncodingrnaznfx1as1promotestheinvasionandproliferationofgastriccancercellsbyregulatinglin28andcapr1n1 |