Reporting of Freshwater Cyanobacterial Poisoning in Terrestrial Wildlife: A Systematic Map

SIMPLE SUMMARY: Harmful cyanobacterial blooms (cyanoHABs) have been reported globally, threatening human and animal health. They are encouraged by the warming climate and agricultural pollution creating nutrient-rich, warm environments, ideal for cyanobacterial proliferation. The cyanotoxins produced by these blooms have caused poisonings in many wildlife species; however, these cases are severely underreported, and many are likely missed. The aim of this systematic map was to collate, organise, and characterise all existing reports of cyanotoxin poisonings in terrestrial wildlife. We conducted a search of the published literature using online databases, yielding a total of 45 cases detailing incidents involving terrestrial wildlife. There is no current standard method for the reporting and diagnosis of cyanotoxin intoxication cases, and we provide recommendations on this to include both clinical diagnostic tools and investigative chemistry techniques. Less than half of all cases employed robust methods of detection and diagnosis based on our recommendations. Most cases were investigated after poisonings had already occurred, and only nine reports mentioned any effort to mitigate the effects of harmful cyanobacteria on terrestrial wildlife. This systematic map details terrestrial wildlife cyanotoxin intoxications from a diagnostic perspective, identifying how reporting can be improved, leading to more successful mitigation and investigative efforts in the future. ABSTRACT: Global warming and over-enrichment of freshwater systems have led to an increase in harmful cyanobacterial blooms (cyanoHABs), affecting human and animal health. The aim of this systematic map was to detail the current literature surrounding cyanotoxin poisonings in terrestrial wildlife and identify possible improvements to reports of morbidity and mortality from cyanotoxins. A systematic search was conducted using the electronic databases Scopus and Web of Science, yielding 5059 published studies identifying 45 separate case reports of wildlife poisonings from North America, Africa, Europe, and Asia. Currently, no gold standard for the diagnosis of cyanotoxin intoxication exists for wildlife, and we present suggested guidelines here. These involved immunoassays and analytical chemistry techniques to identify the toxin involved, PCR to identify the cyanobacterial species involved, and evidence of ingestion or exposure to cyanotoxins in the animals affected. Of the 45 cases, our recommended methods concurred with 48.9% of cases. Most often, cases were investigated after a mortality event had already occurred, and where mitigation was implemented, only three cases were successful in their efforts. Notably, only one case of invasive cyanobacteria was recorded in this review despite invasive species being known to occur throughout the globe; this could explain the underreporting of invasive cyanobacteria. This systematic map highlights the perceived absence of robust detection, surveillance, and diagnosis of cyanotoxin poisoning in wildlife. It may be true that wildlife is less susceptible to these poisoning events; however, the true rates of poisoning are likely much more than is reported in the literature.