The Efficacy of a 3β-Hydroxysteroid Dehydrogenase Inhibitor for the Termination of Mid-Term Pregnancies in Dogs

SIMPLE SUMMARY: The medical termination of unwanted pregnancies in dogs is practiced throughout the world for many reasons, including at the request of animal owners. For these procedures, it is advised to use rapidly effective drugs with minimal side effects. In this study, we investigated trilostane, which decreases progesterone levels, for its efficacy in terminating mid-term pregnancies in dogs, as well as potential side effects. Although trilostane is not a standalone alternative for the termination of pregnancy in dogs, it has been determined that its combined use with another medical agent of known efficacy reduces both the abortion time and potential side effects. Further studies investigating an increased frequency of administration rather than the administration dose could contribute to determining the efficacy of trilostane in dogs. ABSTRACT: Progesterone (P4) is the only hormone needed to maintain pregnancy in dogs. Therefore, a competitive inhibitor of 3β-hydroxysteroid dehydrogenase (3β-HSD) could be a safe and effective option to terminate pregnancy by inhibiting P4 synthesis. To address this hypothesis, we investigated the efficacy of trilostane (TRL), a competitive inhibitor of 3β-HSD, in terminating pregnancy in dogs. Twenty-one dogs between days 30 and 38 of pregnancy were randomly assigned to one of two treatment groups (trilostane (TRL) and aglepristone (AGL)) and an untreated control (CON) group (n = 7 dogs each). Fetal heart rates (FHRs) (measured at 12 h intervals) and serum P4 concentrations (measured at 6 h intervals) were evaluated. The pregnancy termination rates were 0% and 100% in the TRL and AGL groups, respectively. The decrease in the FHR in the TRL and AGL groups was significantly lower than that observed in the CON group. There was a marked decrease in P4 concentrations in the TRL group 6, 54, and 102 h after the initiation of treatment. The luteal expression of StAR appeared to be weaker in the AGL group than the CON group. In conclusion, although a treatment-induced decrease was observed in plasma P4 concentrations, a seven-day TRL treatment alone was not effective in terminating pregnancies. Further studies are needed on the effects of the prolonged administration of TRL with varying doses and frequencies for the termination of mid-term pregnancy in dogs.