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LAMP Coupled CRISPR-Cas12a Module for Rapid, Sensitive and Visual Detection of Porcine Circovirus 2

SIMPLE SUMMARY: In this study, we aimed to establish a visual, rapid, low-cost, sensitive, specific, and portable nucleic acid detection method for PCV2 through coupling LAMP with CRISPR/Cas12a. All the results of LAMP-CRISPR detection, including a low detectable limit of 1 copy/μL, no cross-reactio...

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Detalles Bibliográficos
Autores principales: Lei, Lei, Liao, Fan, Tan, Lei, Duan, Deyong, Zhan, Yang, Wang, Naidong, Wang, Yuge, Peng, Xiaoye, Wang, Kaixin, Huang, Xiaojiu, Yang, Yi, Wang, Aibing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495112/
https://www.ncbi.nlm.nih.gov/pubmed/36139273
http://dx.doi.org/10.3390/ani12182413