Effects of a Novel Gel Formulation of Dog Appeasing Pheromone (DAP) on Behavioral and Physiological Stress Responses in Dogs Undergoing Clinical Examination

SIMPLE SUMMARY: Safeguarding the health of dogs presupposes undergoing regular veterinary visits (VVs). However, the VV can be emotionally challenging, thus impairing the welfare of the patient, also reducing dog and owner compliance. The fear and anxiety experienced by the dogs evokes behavioral and physiological stress responses for adaptive purposes. It is therefore desirable to lower the stress from the visit. With this intent, we tested a gel formulation of a synthetic analogue of the appeasing pheromone secreted by bitches, the dog appeasing pheromone (DAP), as a situational support to improve the perception of the stay in the waiting room and the physical examination. In the waiting room, the dogs exposed to DAP exhibited changes in their behavior, namely significant decrease in lip licking, increase in panting, and nearly significant reduction of low body postures. On the examination table, neither behavioral nor physiological differences were found. DAP did not alter markedly the stress behavior and physiology of dogs during a VV, maybe due to a stress level exceeding the potential efficacy of the product. However, the change of a few stress-associated behaviors suggests that DAP could contribute to improving the welfare of dogs staying in the waiting room before the physical examination. ABSTRACT: The veterinary visit is necessary for safeguarding the health of dogs, but it can be stressful and threaten both the welfare of the patient and the accuracy of the examination. This randomized, triple-blind, placebo-controlled, crossover study aims at evaluating how dog appeasing pheromone (DAP) in a novel gel formulation influences the behavioral and physiological stress responses of 28 dogs undergoing a standardized clinical examination, while staying in the waiting room (WR) and visited in the examination room (ER). Behavioral responses were studied through behavioral categories and subjective scales (WR and ER). Autonomic response considered heart rate (WR and ER), blood pressure (WR and ER), respiratory rate (ER), and rectal temperature (ER). Neuroendocrine response considered salivary cortisol (WR and ER). In the waiting room, the use of DAP was associated with a significant reduction of lip licking (p = 0.0189), an increase in panting (p = 0.0276), and a reduction close to significance (p = 0.0584) of low body postures. No significant differences were observed within the physiological responses. In the examination room, neither behavioral nor physiological differences were found.