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Methanol Extract of Clavularia inflata Exerts Apoptosis and DNA Damage to Oral Cancer Cells
Antiproliferation effects of Clavularia-derived natural products against cancer cells have been reported on, but most studies have focused on identifying bioactive compounds, lacking a detailed investigation of the molecular mechanism. Crude extracts generally exhibit multiple targeting potentials f...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495492/ https://www.ncbi.nlm.nih.gov/pubmed/36139851 http://dx.doi.org/10.3390/antiox11091777 |
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author | Hsu, Yin-Yin Chuang, Ya-Ting Yen, Ching-Yu Cheng, Ming-Ya Chen, Ching-Yeu Cheng, Yuan-Bin Chang, Hsueh-Wei |
author_facet | Hsu, Yin-Yin Chuang, Ya-Ting Yen, Ching-Yu Cheng, Ming-Ya Chen, Ching-Yeu Cheng, Yuan-Bin Chang, Hsueh-Wei |
author_sort | Hsu, Yin-Yin |
collection | PubMed |
description | Antiproliferation effects of Clavularia-derived natural products against cancer cells have been reported on, but most studies have focused on identifying bioactive compounds, lacking a detailed investigation of the molecular mechanism. Crude extracts generally exhibit multiple targeting potentials for anticancer effects, but they have rarely been assessed for methanol extracts of Clavularia inflata (MECI). This investigation aims to evaluate the antiproliferation of MECI and to examine several potential mechanisms between oral cancer and normal cells. A 24 h MTS assay demonstrated that MECI decreased cell viability in several oral cancer cell lines more than in normal cells. N-acetylcysteine (NAC), an oxidative stress inhibitor, recovered these antiproliferation effects. Higher oxidative stress was stimulated by MECI in oral cancer cells than in normal cells, as proven by examining reactive oxygen species and mitochondrial superoxide. This preferential induction of oxidative stress was partly explained by downregulating more cellular antioxidants, such as glutathione, in oral cancer cells than in normal cells. Consequently, the MECI-generated high oxidative stress in oral cancer cells was preferred to trigger more subG1 population, apoptosis expression (annexin V and caspase activation), and DNA damage, reverted by NAC. In conclusion, MECI is a potent marine natural product showing preferential antiproliferation against oral cancer cells. |
format | Online Article Text |
id | pubmed-9495492 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94954922022-09-23 Methanol Extract of Clavularia inflata Exerts Apoptosis and DNA Damage to Oral Cancer Cells Hsu, Yin-Yin Chuang, Ya-Ting Yen, Ching-Yu Cheng, Ming-Ya Chen, Ching-Yeu Cheng, Yuan-Bin Chang, Hsueh-Wei Antioxidants (Basel) Article Antiproliferation effects of Clavularia-derived natural products against cancer cells have been reported on, but most studies have focused on identifying bioactive compounds, lacking a detailed investigation of the molecular mechanism. Crude extracts generally exhibit multiple targeting potentials for anticancer effects, but they have rarely been assessed for methanol extracts of Clavularia inflata (MECI). This investigation aims to evaluate the antiproliferation of MECI and to examine several potential mechanisms between oral cancer and normal cells. A 24 h MTS assay demonstrated that MECI decreased cell viability in several oral cancer cell lines more than in normal cells. N-acetylcysteine (NAC), an oxidative stress inhibitor, recovered these antiproliferation effects. Higher oxidative stress was stimulated by MECI in oral cancer cells than in normal cells, as proven by examining reactive oxygen species and mitochondrial superoxide. This preferential induction of oxidative stress was partly explained by downregulating more cellular antioxidants, such as glutathione, in oral cancer cells than in normal cells. Consequently, the MECI-generated high oxidative stress in oral cancer cells was preferred to trigger more subG1 population, apoptosis expression (annexin V and caspase activation), and DNA damage, reverted by NAC. In conclusion, MECI is a potent marine natural product showing preferential antiproliferation against oral cancer cells. MDPI 2022-09-08 /pmc/articles/PMC9495492/ /pubmed/36139851 http://dx.doi.org/10.3390/antiox11091777 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hsu, Yin-Yin Chuang, Ya-Ting Yen, Ching-Yu Cheng, Ming-Ya Chen, Ching-Yeu Cheng, Yuan-Bin Chang, Hsueh-Wei Methanol Extract of Clavularia inflata Exerts Apoptosis and DNA Damage to Oral Cancer Cells |
title | Methanol Extract of Clavularia inflata Exerts Apoptosis and DNA Damage to Oral Cancer Cells |
title_full | Methanol Extract of Clavularia inflata Exerts Apoptosis and DNA Damage to Oral Cancer Cells |
title_fullStr | Methanol Extract of Clavularia inflata Exerts Apoptosis and DNA Damage to Oral Cancer Cells |
title_full_unstemmed | Methanol Extract of Clavularia inflata Exerts Apoptosis and DNA Damage to Oral Cancer Cells |
title_short | Methanol Extract of Clavularia inflata Exerts Apoptosis and DNA Damage to Oral Cancer Cells |
title_sort | methanol extract of clavularia inflata exerts apoptosis and dna damage to oral cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495492/ https://www.ncbi.nlm.nih.gov/pubmed/36139851 http://dx.doi.org/10.3390/antiox11091777 |
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