Prediction of methane per unit of dry matter intake in growing and finishing cattle from the ratio of dietary concentrations of starch to neutral detergent fiber alone or in combination with dietary concentration of ether extract

Previous research demonstrated that a fixed value of 0.2433 (SE = 0.0134) Mcal of CH(4)/kg of dry matter intake (DMI) could be used to predict CH(4) production with accuracy and precision on par with similar equations in the literature. Slope bias was substantially less for the fixed-coefficient equation than noted for the other DMI- or gross energy intake (GEI)-based equations, but mean bias was substantially greater, presumably reflecting the failure of the fixed-coefficient approach to account for dietary factors that affect CH(4) production. In this article, we report on the use of the dietary ratio of concentrations of starch to neutral detergent fiber (NDF) and dietary ether extract (EE) concentration to improve the accuracy and precision of the fixed-coefficient equation. The same development data set used to create the fixed-coefficient equation was used in the present study, which included 134 treatment means from 34 respiration calorimetry studies. Based on stepwise regression with dietary NDF, starch, crude protein, EE, and the starch:NDF ratio as possible dependent variables, the starch:NDF ratio and EE were the only dietary variables selected (P ≤ 0.15). The study-adjusted relationship with the starch:NDF ratio (r(2) = 0.673; root mean square error [RMSE] = 0.0327) was: Mcal of CH(4)/kg of DMI = 0.2883 − 0.03474 × starch:NDF; whereas the relationship with a model that included both starch:NDF ratio and dietary EE (r(2) = 0.738; RMSE = 0.0315) was: Mcal of CH(4)/kg of DMI = 0.3227 − 0.0334 × starch:NDF − 0.00868 × % EE. A previously published independent data set with 129 treatment means from 30 respiration calorimetry studies was used to evaluate these two equations, along with two additional equations in which g/d of CH(4) was predicted directly from DMI, starch:NDF ratio, and/or dietary EE. The two Mcal of CH(4)/kg of DMI equations had superior fit statistics to the previously published 0.2433 Mcal of CH(4)/kg of DMI equation, with a substantial decrease in mean bias and improved concordance correlation coefficients. Moreover, the Mcal of CH(4)/kg of DMI equations resulted in improved fit relative to direct prediction of g/d of CH(4) from DMI, the starch:NDF ratio, and % EE. Based on these results, further evaluation of the dietary ratio of starch-to-NDF concentrations and EE concentration to predict methane production per unit DMI in beef cattle is warranted.