Cargando…

Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs

Synthetic hydrogels from polyisocyanides (PIC) are a type of novel thermoreversible biomaterials, which can covalently bind biomolecules such as adhesion peptides to provide a suitable extracellular matrix (ECM)-like microenvironment for different cells. Although we have demonstrated that PIC is sui...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Chiyuan, Liu, Kaizheng, Li, Qin, Xiong, Yue, Xu, Cuixiang, Zhang, Wenya, Ruan, Changshun, Li, Xin, Lei, Xiaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495567/
https://www.ncbi.nlm.nih.gov/pubmed/36134999
http://dx.doi.org/10.3390/bioengineering9090453
_version_ 1784794050000322560
author Ma, Chiyuan
Liu, Kaizheng
Li, Qin
Xiong, Yue
Xu, Cuixiang
Zhang, Wenya
Ruan, Changshun
Li, Xin
Lei, Xiaohua
author_facet Ma, Chiyuan
Liu, Kaizheng
Li, Qin
Xiong, Yue
Xu, Cuixiang
Zhang, Wenya
Ruan, Changshun
Li, Xin
Lei, Xiaohua
author_sort Ma, Chiyuan
collection PubMed
description Synthetic hydrogels from polyisocyanides (PIC) are a type of novel thermoreversible biomaterials, which can covalently bind biomolecules such as adhesion peptides to provide a suitable extracellular matrix (ECM)-like microenvironment for different cells. Although we have demonstrated that PIC is suitable for three-dimensional (3D) culture of several cell types, it is unknown whether this hydrogel sustains the proliferation and passaging of cells originating from different germ layers. In the present study, we propose a 3D culture system for three representative cell sources: Schwann cells (ectoderm), hepatocytes (endoderm), and endothelial cells (mesoderm). Both Schwann cells and hepatocytes proliferated into multicellular spheroids and maintained their properties, regardless of the amount of cell-adhesive RGD motifs in long-term culture. Notably, Schwann cells grew into larger spheroids in RGD-free PIC than in PIC-RGD, while HL-7702 showed the opposite behavior. Endothelial cells (human umbilical vein endothelial cells, HUVECs) spread and formed an endothelial cell (EC) network only in PIC-RGD. Moreover, in a hepatocyte/HUVEC co-culture system, the characteristics of both cells were well kept for a long period in PIC-RGD. In all, our work highlights a simple ECM mimic that supports the growth and phenotype maintenance of cells from all germ layers in the long term. Our findings might contribute to research on biological development, organoid engineering, and in vitro drug screening.
format Online
Article
Text
id pubmed-9495567
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94955672022-09-23 Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs Ma, Chiyuan Liu, Kaizheng Li, Qin Xiong, Yue Xu, Cuixiang Zhang, Wenya Ruan, Changshun Li, Xin Lei, Xiaohua Bioengineering (Basel) Article Synthetic hydrogels from polyisocyanides (PIC) are a type of novel thermoreversible biomaterials, which can covalently bind biomolecules such as adhesion peptides to provide a suitable extracellular matrix (ECM)-like microenvironment for different cells. Although we have demonstrated that PIC is suitable for three-dimensional (3D) culture of several cell types, it is unknown whether this hydrogel sustains the proliferation and passaging of cells originating from different germ layers. In the present study, we propose a 3D culture system for three representative cell sources: Schwann cells (ectoderm), hepatocytes (endoderm), and endothelial cells (mesoderm). Both Schwann cells and hepatocytes proliferated into multicellular spheroids and maintained their properties, regardless of the amount of cell-adhesive RGD motifs in long-term culture. Notably, Schwann cells grew into larger spheroids in RGD-free PIC than in PIC-RGD, while HL-7702 showed the opposite behavior. Endothelial cells (human umbilical vein endothelial cells, HUVECs) spread and formed an endothelial cell (EC) network only in PIC-RGD. Moreover, in a hepatocyte/HUVEC co-culture system, the characteristics of both cells were well kept for a long period in PIC-RGD. In all, our work highlights a simple ECM mimic that supports the growth and phenotype maintenance of cells from all germ layers in the long term. Our findings might contribute to research on biological development, organoid engineering, and in vitro drug screening. MDPI 2022-09-08 /pmc/articles/PMC9495567/ /pubmed/36134999 http://dx.doi.org/10.3390/bioengineering9090453 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Chiyuan
Liu, Kaizheng
Li, Qin
Xiong, Yue
Xu, Cuixiang
Zhang, Wenya
Ruan, Changshun
Li, Xin
Lei, Xiaohua
Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs
title Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs
title_full Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs
title_fullStr Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs
title_full_unstemmed Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs
title_short Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs
title_sort synthetic extracellular matrices for 3d culture of schwann cells, hepatocytes, and huvecs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495567/
https://www.ncbi.nlm.nih.gov/pubmed/36134999
http://dx.doi.org/10.3390/bioengineering9090453
work_keys_str_mv AT machiyuan syntheticextracellularmatricesfor3dcultureofschwanncellshepatocytesandhuvecs
AT liukaizheng syntheticextracellularmatricesfor3dcultureofschwanncellshepatocytesandhuvecs
AT liqin syntheticextracellularmatricesfor3dcultureofschwanncellshepatocytesandhuvecs
AT xiongyue syntheticextracellularmatricesfor3dcultureofschwanncellshepatocytesandhuvecs
AT xucuixiang syntheticextracellularmatricesfor3dcultureofschwanncellshepatocytesandhuvecs
AT zhangwenya syntheticextracellularmatricesfor3dcultureofschwanncellshepatocytesandhuvecs
AT ruanchangshun syntheticextracellularmatricesfor3dcultureofschwanncellshepatocytesandhuvecs
AT lixin syntheticextracellularmatricesfor3dcultureofschwanncellshepatocytesandhuvecs
AT leixiaohua syntheticextracellularmatricesfor3dcultureofschwanncellshepatocytesandhuvecs