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Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs
Synthetic hydrogels from polyisocyanides (PIC) are a type of novel thermoreversible biomaterials, which can covalently bind biomolecules such as adhesion peptides to provide a suitable extracellular matrix (ECM)-like microenvironment for different cells. Although we have demonstrated that PIC is sui...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495567/ https://www.ncbi.nlm.nih.gov/pubmed/36134999 http://dx.doi.org/10.3390/bioengineering9090453 |
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author | Ma, Chiyuan Liu, Kaizheng Li, Qin Xiong, Yue Xu, Cuixiang Zhang, Wenya Ruan, Changshun Li, Xin Lei, Xiaohua |
author_facet | Ma, Chiyuan Liu, Kaizheng Li, Qin Xiong, Yue Xu, Cuixiang Zhang, Wenya Ruan, Changshun Li, Xin Lei, Xiaohua |
author_sort | Ma, Chiyuan |
collection | PubMed |
description | Synthetic hydrogels from polyisocyanides (PIC) are a type of novel thermoreversible biomaterials, which can covalently bind biomolecules such as adhesion peptides to provide a suitable extracellular matrix (ECM)-like microenvironment for different cells. Although we have demonstrated that PIC is suitable for three-dimensional (3D) culture of several cell types, it is unknown whether this hydrogel sustains the proliferation and passaging of cells originating from different germ layers. In the present study, we propose a 3D culture system for three representative cell sources: Schwann cells (ectoderm), hepatocytes (endoderm), and endothelial cells (mesoderm). Both Schwann cells and hepatocytes proliferated into multicellular spheroids and maintained their properties, regardless of the amount of cell-adhesive RGD motifs in long-term culture. Notably, Schwann cells grew into larger spheroids in RGD-free PIC than in PIC-RGD, while HL-7702 showed the opposite behavior. Endothelial cells (human umbilical vein endothelial cells, HUVECs) spread and formed an endothelial cell (EC) network only in PIC-RGD. Moreover, in a hepatocyte/HUVEC co-culture system, the characteristics of both cells were well kept for a long period in PIC-RGD. In all, our work highlights a simple ECM mimic that supports the growth and phenotype maintenance of cells from all germ layers in the long term. Our findings might contribute to research on biological development, organoid engineering, and in vitro drug screening. |
format | Online Article Text |
id | pubmed-9495567 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94955672022-09-23 Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs Ma, Chiyuan Liu, Kaizheng Li, Qin Xiong, Yue Xu, Cuixiang Zhang, Wenya Ruan, Changshun Li, Xin Lei, Xiaohua Bioengineering (Basel) Article Synthetic hydrogels from polyisocyanides (PIC) are a type of novel thermoreversible biomaterials, which can covalently bind biomolecules such as adhesion peptides to provide a suitable extracellular matrix (ECM)-like microenvironment for different cells. Although we have demonstrated that PIC is suitable for three-dimensional (3D) culture of several cell types, it is unknown whether this hydrogel sustains the proliferation and passaging of cells originating from different germ layers. In the present study, we propose a 3D culture system for three representative cell sources: Schwann cells (ectoderm), hepatocytes (endoderm), and endothelial cells (mesoderm). Both Schwann cells and hepatocytes proliferated into multicellular spheroids and maintained their properties, regardless of the amount of cell-adhesive RGD motifs in long-term culture. Notably, Schwann cells grew into larger spheroids in RGD-free PIC than in PIC-RGD, while HL-7702 showed the opposite behavior. Endothelial cells (human umbilical vein endothelial cells, HUVECs) spread and formed an endothelial cell (EC) network only in PIC-RGD. Moreover, in a hepatocyte/HUVEC co-culture system, the characteristics of both cells were well kept for a long period in PIC-RGD. In all, our work highlights a simple ECM mimic that supports the growth and phenotype maintenance of cells from all germ layers in the long term. Our findings might contribute to research on biological development, organoid engineering, and in vitro drug screening. MDPI 2022-09-08 /pmc/articles/PMC9495567/ /pubmed/36134999 http://dx.doi.org/10.3390/bioengineering9090453 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ma, Chiyuan Liu, Kaizheng Li, Qin Xiong, Yue Xu, Cuixiang Zhang, Wenya Ruan, Changshun Li, Xin Lei, Xiaohua Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs |
title | Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs |
title_full | Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs |
title_fullStr | Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs |
title_full_unstemmed | Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs |
title_short | Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs |
title_sort | synthetic extracellular matrices for 3d culture of schwann cells, hepatocytes, and huvecs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495567/ https://www.ncbi.nlm.nih.gov/pubmed/36134999 http://dx.doi.org/10.3390/bioengineering9090453 |
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