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Metabolite Profiling Analysis and the Correlation with Biological Activity of Betalain-Rich Portulaca grandiflora Hook. Extracts

The aim of the study was to evaluate the possible correlation between the bioactivity and the phytochemical profile of four betalain-rich extracts from Portulaca grandiflora Hook. The HPLC-DAD-ESI-MS analysis indicated the presence of 19 betaxanthins and two betacyanins. The highest concentrations o...

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Autores principales: Spórna-Kucab, Aneta, Tekieli, Anna, Grzegorczyk, Agnieszka, Świątek, Łukasz, Rajtar, Barbara, Skalicka-Woźniak, Krystyna, Starzak, Karolina, Nemzer, Boris, Pietrzkowski, Zbigniew, Wybraniec, Sławomir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495615/
https://www.ncbi.nlm.nih.gov/pubmed/36139728
http://dx.doi.org/10.3390/antiox11091654
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author Spórna-Kucab, Aneta
Tekieli, Anna
Grzegorczyk, Agnieszka
Świątek, Łukasz
Rajtar, Barbara
Skalicka-Woźniak, Krystyna
Starzak, Karolina
Nemzer, Boris
Pietrzkowski, Zbigniew
Wybraniec, Sławomir
author_facet Spórna-Kucab, Aneta
Tekieli, Anna
Grzegorczyk, Agnieszka
Świątek, Łukasz
Rajtar, Barbara
Skalicka-Woźniak, Krystyna
Starzak, Karolina
Nemzer, Boris
Pietrzkowski, Zbigniew
Wybraniec, Sławomir
author_sort Spórna-Kucab, Aneta
collection PubMed
description The aim of the study was to evaluate the possible correlation between the bioactivity and the phytochemical profile of four betalain-rich extracts from Portulaca grandiflora Hook. The HPLC-DAD-ESI-MS analysis indicated the presence of 19 betaxanthins and two betacyanins. The highest concentrations of betaxanthins (982 mg/100 g DE) and betacyanins (650 mg/100 g DE) were noticed in orange and purple flowers extracts, respectively. The HPLC-DAD-ESI-HRMS/MS analyses revealed the presence of a total of 71 compounds. Fifteen new betaxanthins and fifty other metabolites were identified for the first time. The antioxidant activity of the studied flower extracts increased in the sequence of yellow < orange < purple < red (0.066–0.176 mM TE/g DE). Betalains showed less effect on the antioxidant activity of extracts than other metabolites did. Extracts from yellow and orange flowers were more active against Gram-positive bacteria (MIC = 4–16 mg/L), whereas extracts from red and purple flowers were slightly more active against Gram-negative bacteria (MIC = 16–32 mg/L). All the extracts showed the same activity against yeasts (MIC = 32 mg/L). Betaxanthins were active against Gram-positive bacteria, whereas betacyanins were active against Gram-negative bacteria. Remaining metabolites also exhibited antimicrobial activities. The cytotoxicity assessment showed that the P. grandiflora extracts were non-toxic to normal VERO cells. No significant antiviral activity towards Human Herpesvirus type 1 was observed (62 µg/mL). Among the tested varieties, the purple one showed anticancer selectivity towards colon carcinoma cells (RKO).
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spelling pubmed-94956152022-09-23 Metabolite Profiling Analysis and the Correlation with Biological Activity of Betalain-Rich Portulaca grandiflora Hook. Extracts Spórna-Kucab, Aneta Tekieli, Anna Grzegorczyk, Agnieszka Świątek, Łukasz Rajtar, Barbara Skalicka-Woźniak, Krystyna Starzak, Karolina Nemzer, Boris Pietrzkowski, Zbigniew Wybraniec, Sławomir Antioxidants (Basel) Article The aim of the study was to evaluate the possible correlation between the bioactivity and the phytochemical profile of four betalain-rich extracts from Portulaca grandiflora Hook. The HPLC-DAD-ESI-MS analysis indicated the presence of 19 betaxanthins and two betacyanins. The highest concentrations of betaxanthins (982 mg/100 g DE) and betacyanins (650 mg/100 g DE) were noticed in orange and purple flowers extracts, respectively. The HPLC-DAD-ESI-HRMS/MS analyses revealed the presence of a total of 71 compounds. Fifteen new betaxanthins and fifty other metabolites were identified for the first time. The antioxidant activity of the studied flower extracts increased in the sequence of yellow < orange < purple < red (0.066–0.176 mM TE/g DE). Betalains showed less effect on the antioxidant activity of extracts than other metabolites did. Extracts from yellow and orange flowers were more active against Gram-positive bacteria (MIC = 4–16 mg/L), whereas extracts from red and purple flowers were slightly more active against Gram-negative bacteria (MIC = 16–32 mg/L). All the extracts showed the same activity against yeasts (MIC = 32 mg/L). Betaxanthins were active against Gram-positive bacteria, whereas betacyanins were active against Gram-negative bacteria. Remaining metabolites also exhibited antimicrobial activities. The cytotoxicity assessment showed that the P. grandiflora extracts were non-toxic to normal VERO cells. No significant antiviral activity towards Human Herpesvirus type 1 was observed (62 µg/mL). Among the tested varieties, the purple one showed anticancer selectivity towards colon carcinoma cells (RKO). MDPI 2022-08-25 /pmc/articles/PMC9495615/ /pubmed/36139728 http://dx.doi.org/10.3390/antiox11091654 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Spórna-Kucab, Aneta
Tekieli, Anna
Grzegorczyk, Agnieszka
Świątek, Łukasz
Rajtar, Barbara
Skalicka-Woźniak, Krystyna
Starzak, Karolina
Nemzer, Boris
Pietrzkowski, Zbigniew
Wybraniec, Sławomir
Metabolite Profiling Analysis and the Correlation with Biological Activity of Betalain-Rich Portulaca grandiflora Hook. Extracts
title Metabolite Profiling Analysis and the Correlation with Biological Activity of Betalain-Rich Portulaca grandiflora Hook. Extracts
title_full Metabolite Profiling Analysis and the Correlation with Biological Activity of Betalain-Rich Portulaca grandiflora Hook. Extracts
title_fullStr Metabolite Profiling Analysis and the Correlation with Biological Activity of Betalain-Rich Portulaca grandiflora Hook. Extracts
title_full_unstemmed Metabolite Profiling Analysis and the Correlation with Biological Activity of Betalain-Rich Portulaca grandiflora Hook. Extracts
title_short Metabolite Profiling Analysis and the Correlation with Biological Activity of Betalain-Rich Portulaca grandiflora Hook. Extracts
title_sort metabolite profiling analysis and the correlation with biological activity of betalain-rich portulaca grandiflora hook. extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495615/
https://www.ncbi.nlm.nih.gov/pubmed/36139728
http://dx.doi.org/10.3390/antiox11091654
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