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Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering

Nerve injuries remain clinically challenging, and allografts showed great promise. Decellularized nerve allografts possess excellent biocompatibility and biological activity. However, the vast majority of decellularization protocols were established for small-size rodent nerves and are not suitable...

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Autores principales: Hopf, Alois, Al-Bayati, Lina, Schaefer, Dirk J., Kalbermatten, Daniel F., Guzman, Raphael, Madduri, Srinivas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495622/
https://www.ncbi.nlm.nih.gov/pubmed/36134958
http://dx.doi.org/10.3390/bioengineering9090412
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author Hopf, Alois
Al-Bayati, Lina
Schaefer, Dirk J.
Kalbermatten, Daniel F.
Guzman, Raphael
Madduri, Srinivas
author_facet Hopf, Alois
Al-Bayati, Lina
Schaefer, Dirk J.
Kalbermatten, Daniel F.
Guzman, Raphael
Madduri, Srinivas
author_sort Hopf, Alois
collection PubMed
description Nerve injuries remain clinically challenging, and allografts showed great promise. Decellularized nerve allografts possess excellent biocompatibility and biological activity. However, the vast majority of decellularization protocols were established for small-size rodent nerves and are not suitable for clinical application. We aimed at developing a new method of decellularizing large-diameter nerves suitable for human transplantation. Repeated rounds of optimization to remove immunogenic material and preserve the extracellular structure were applied to the porcine sciatic nerve. Following optimization, extensive in vitro analysis of the acellular grafts via immunocytochemistry, immunohistology, proteomics and cell transplantation studies were performed. Large segments (up to 8 cm) of the porcine sciatic nerve were efficiently decellularized and histology, microscopy and proteomics analysis showed sufficient preservation of the extracellular matrix, with simultaneous consistent removal of immunogenic material such as myelin, DNA and axons, and axonal growth inhibitory molecules. Cell studies also demonstrated the suitability of these acellular grafts for 3D cell culture studies and translation to future large animal studies and clinical trials. By using non-human donors for peripheral nerve transplantation, significant drawbacks associated with the gold standard can be eliminated while simultaneously preserving the beneficial features of the extracellular matrix.
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spelling pubmed-94956222022-09-23 Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering Hopf, Alois Al-Bayati, Lina Schaefer, Dirk J. Kalbermatten, Daniel F. Guzman, Raphael Madduri, Srinivas Bioengineering (Basel) Article Nerve injuries remain clinically challenging, and allografts showed great promise. Decellularized nerve allografts possess excellent biocompatibility and biological activity. However, the vast majority of decellularization protocols were established for small-size rodent nerves and are not suitable for clinical application. We aimed at developing a new method of decellularizing large-diameter nerves suitable for human transplantation. Repeated rounds of optimization to remove immunogenic material and preserve the extracellular structure were applied to the porcine sciatic nerve. Following optimization, extensive in vitro analysis of the acellular grafts via immunocytochemistry, immunohistology, proteomics and cell transplantation studies were performed. Large segments (up to 8 cm) of the porcine sciatic nerve were efficiently decellularized and histology, microscopy and proteomics analysis showed sufficient preservation of the extracellular matrix, with simultaneous consistent removal of immunogenic material such as myelin, DNA and axons, and axonal growth inhibitory molecules. Cell studies also demonstrated the suitability of these acellular grafts for 3D cell culture studies and translation to future large animal studies and clinical trials. By using non-human donors for peripheral nerve transplantation, significant drawbacks associated with the gold standard can be eliminated while simultaneously preserving the beneficial features of the extracellular matrix. MDPI 2022-08-24 /pmc/articles/PMC9495622/ /pubmed/36134958 http://dx.doi.org/10.3390/bioengineering9090412 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hopf, Alois
Al-Bayati, Lina
Schaefer, Dirk J.
Kalbermatten, Daniel F.
Guzman, Raphael
Madduri, Srinivas
Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering
title Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering
title_full Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering
title_fullStr Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering
title_full_unstemmed Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering
title_short Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering
title_sort optimized decellularization protocol for large peripheral nerve segments: towards personalized nerve bioengineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495622/
https://www.ncbi.nlm.nih.gov/pubmed/36134958
http://dx.doi.org/10.3390/bioengineering9090412
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