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Physiological and Comparative Transcriptome Analysis Reveals the Mechanism by Which Exogenous 24-Epibrassinolide Application Enhances Drought Resistance in Potato (Solanum tuberosum L.)
Drought stress is a key factor limiting the growth and tuber yield of potatoes (Solanum tuberosum L.). Brassinosteroids (BRs) have been shown to alleviate drought stress in several plant species; however, little is known about the physiological and molecular mechanisms by which BRs enhance drought r...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495798/ https://www.ncbi.nlm.nih.gov/pubmed/36139774 http://dx.doi.org/10.3390/antiox11091701 |
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author | Zheng, Hao Ma, Jie Huang, Wenli Di, Hongmei Xia, Xue Ma, Wei Ma, Jun Yang, Jiao Li, Xiaomei Lian, Huashan Huang, Zhi Tang, Yi Zheng, Yangxia Li, Huanxiu Zhang, Fen Sun, Bo |
author_facet | Zheng, Hao Ma, Jie Huang, Wenli Di, Hongmei Xia, Xue Ma, Wei Ma, Jun Yang, Jiao Li, Xiaomei Lian, Huashan Huang, Zhi Tang, Yi Zheng, Yangxia Li, Huanxiu Zhang, Fen Sun, Bo |
author_sort | Zheng, Hao |
collection | PubMed |
description | Drought stress is a key factor limiting the growth and tuber yield of potatoes (Solanum tuberosum L.). Brassinosteroids (BRs) have been shown to alleviate drought stress in several plant species; however, little is known about the physiological and molecular mechanisms by which BRs enhance drought resistance in potatoes. Here, we characterized changes in the physiology and transcriptome of the tetraploid potato variety ‘Xuanshu-2′ in response to drought stress after 24-epibrassinolide (EBR) pretreatment. The abscisic acid (ABA) content, photosynthetic capacity, and the activities of antioxidant enzymes were increased; the intercellular CO(2) concentration, relative conductivity, reactive oxygen species, malondialdehyde, proline, and soluble sugar content were decreased after EBR pretreatment compared with plants under drought stress. Transcriptome analysis revealed 1330 differently expressed genes (DEGs) involved in the response to drought stress after EBR pretreatment. DEGs were enriched in plant hormone signal transduction, starch and sucrose metabolism, circadian rhythm, flavonoid biosynthesis, and carotenoid biosynthesis. DEGs associated with the BR signaling and biosynthesis pathways, as well as ABA metabolic pathways were identified. Our findings provide new insights into the mechanisms by which BRs enhance the drought resistance of potatoes. |
format | Online Article Text |
id | pubmed-9495798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94957982022-09-23 Physiological and Comparative Transcriptome Analysis Reveals the Mechanism by Which Exogenous 24-Epibrassinolide Application Enhances Drought Resistance in Potato (Solanum tuberosum L.) Zheng, Hao Ma, Jie Huang, Wenli Di, Hongmei Xia, Xue Ma, Wei Ma, Jun Yang, Jiao Li, Xiaomei Lian, Huashan Huang, Zhi Tang, Yi Zheng, Yangxia Li, Huanxiu Zhang, Fen Sun, Bo Antioxidants (Basel) Article Drought stress is a key factor limiting the growth and tuber yield of potatoes (Solanum tuberosum L.). Brassinosteroids (BRs) have been shown to alleviate drought stress in several plant species; however, little is known about the physiological and molecular mechanisms by which BRs enhance drought resistance in potatoes. Here, we characterized changes in the physiology and transcriptome of the tetraploid potato variety ‘Xuanshu-2′ in response to drought stress after 24-epibrassinolide (EBR) pretreatment. The abscisic acid (ABA) content, photosynthetic capacity, and the activities of antioxidant enzymes were increased; the intercellular CO(2) concentration, relative conductivity, reactive oxygen species, malondialdehyde, proline, and soluble sugar content were decreased after EBR pretreatment compared with plants under drought stress. Transcriptome analysis revealed 1330 differently expressed genes (DEGs) involved in the response to drought stress after EBR pretreatment. DEGs were enriched in plant hormone signal transduction, starch and sucrose metabolism, circadian rhythm, flavonoid biosynthesis, and carotenoid biosynthesis. DEGs associated with the BR signaling and biosynthesis pathways, as well as ABA metabolic pathways were identified. Our findings provide new insights into the mechanisms by which BRs enhance the drought resistance of potatoes. MDPI 2022-08-30 /pmc/articles/PMC9495798/ /pubmed/36139774 http://dx.doi.org/10.3390/antiox11091701 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zheng, Hao Ma, Jie Huang, Wenli Di, Hongmei Xia, Xue Ma, Wei Ma, Jun Yang, Jiao Li, Xiaomei Lian, Huashan Huang, Zhi Tang, Yi Zheng, Yangxia Li, Huanxiu Zhang, Fen Sun, Bo Physiological and Comparative Transcriptome Analysis Reveals the Mechanism by Which Exogenous 24-Epibrassinolide Application Enhances Drought Resistance in Potato (Solanum tuberosum L.) |
title | Physiological and Comparative Transcriptome Analysis Reveals the Mechanism by Which Exogenous 24-Epibrassinolide Application Enhances Drought Resistance in Potato (Solanum tuberosum L.) |
title_full | Physiological and Comparative Transcriptome Analysis Reveals the Mechanism by Which Exogenous 24-Epibrassinolide Application Enhances Drought Resistance in Potato (Solanum tuberosum L.) |
title_fullStr | Physiological and Comparative Transcriptome Analysis Reveals the Mechanism by Which Exogenous 24-Epibrassinolide Application Enhances Drought Resistance in Potato (Solanum tuberosum L.) |
title_full_unstemmed | Physiological and Comparative Transcriptome Analysis Reveals the Mechanism by Which Exogenous 24-Epibrassinolide Application Enhances Drought Resistance in Potato (Solanum tuberosum L.) |
title_short | Physiological and Comparative Transcriptome Analysis Reveals the Mechanism by Which Exogenous 24-Epibrassinolide Application Enhances Drought Resistance in Potato (Solanum tuberosum L.) |
title_sort | physiological and comparative transcriptome analysis reveals the mechanism by which exogenous 24-epibrassinolide application enhances drought resistance in potato (solanum tuberosum l.) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495798/ https://www.ncbi.nlm.nih.gov/pubmed/36139774 http://dx.doi.org/10.3390/antiox11091701 |
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