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The Aftermath of Long-Term Cigarette Smoking on Telomere Length and Mitochondrial DNA Copy Number in Human Cumulus Cells Prior to In Vitro Fertilization—A Pilot Study

Cigarette smoking among women of reproductive age is known to take a toll on systemic health and fertility potential by severely impacting ovarian tissues and cells, such as granulosa and cumulus cells (CCs). The purpose of this study was to determine the potential damage caused by tobacco smoke at...

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Autores principales: Konstantinidou, Fani, Budani, Maria Cristina, Marconi, Guya Diletta, Gonnella, Francesca, Sarra, Annalina, Trubiani, Oriana, Stuppia, Liborio, Tiboni, Gian Mario, Gatta, Valentina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495883/
https://www.ncbi.nlm.nih.gov/pubmed/36139914
http://dx.doi.org/10.3390/antiox11091841
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author Konstantinidou, Fani
Budani, Maria Cristina
Marconi, Guya Diletta
Gonnella, Francesca
Sarra, Annalina
Trubiani, Oriana
Stuppia, Liborio
Tiboni, Gian Mario
Gatta, Valentina
author_facet Konstantinidou, Fani
Budani, Maria Cristina
Marconi, Guya Diletta
Gonnella, Francesca
Sarra, Annalina
Trubiani, Oriana
Stuppia, Liborio
Tiboni, Gian Mario
Gatta, Valentina
author_sort Konstantinidou, Fani
collection PubMed
description Cigarette smoking among women of reproductive age is known to take a toll on systemic health and fertility potential by severely impacting ovarian tissues and cells, such as granulosa and cumulus cells (CCs). The purpose of this study was to determine the potential damage caused by tobacco smoke at a molecular level in the CCs of females who had undergone in vitro fertilization. The level of intracellular damage was determined by estimating the average telomere length (TL) and mitochondrial DNA copy number (mtDNA-CN), as well as the expression profile of telomere maintenance genes TERF1, TERF2, POT1 and microRNAs miR-155, miR-23a and miR-185. Western blotting analysis was performed to detect consequent protein levels of TERF1, TERF2 and POT1. Our results evidenced significantly lower relative TL and mtDNA-CN and a down-regulation pattern for all three described genes and corresponding proteins in the CCs of smokers compared with controls (p < 0.05). No significant differences were found in the miRNAs’ modulation. Combined, our data add another piece to the puzzle of the complex regulatory molecular networks controlling the general effects of tobacco smoke in CCs. This pilot study extends the until now modest number of studies simultaneously investigating the mtDNA-CN and TL pathways in the human CCs of smoking women.
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spelling pubmed-94958832022-09-23 The Aftermath of Long-Term Cigarette Smoking on Telomere Length and Mitochondrial DNA Copy Number in Human Cumulus Cells Prior to In Vitro Fertilization—A Pilot Study Konstantinidou, Fani Budani, Maria Cristina Marconi, Guya Diletta Gonnella, Francesca Sarra, Annalina Trubiani, Oriana Stuppia, Liborio Tiboni, Gian Mario Gatta, Valentina Antioxidants (Basel) Article Cigarette smoking among women of reproductive age is known to take a toll on systemic health and fertility potential by severely impacting ovarian tissues and cells, such as granulosa and cumulus cells (CCs). The purpose of this study was to determine the potential damage caused by tobacco smoke at a molecular level in the CCs of females who had undergone in vitro fertilization. The level of intracellular damage was determined by estimating the average telomere length (TL) and mitochondrial DNA copy number (mtDNA-CN), as well as the expression profile of telomere maintenance genes TERF1, TERF2, POT1 and microRNAs miR-155, miR-23a and miR-185. Western blotting analysis was performed to detect consequent protein levels of TERF1, TERF2 and POT1. Our results evidenced significantly lower relative TL and mtDNA-CN and a down-regulation pattern for all three described genes and corresponding proteins in the CCs of smokers compared with controls (p < 0.05). No significant differences were found in the miRNAs’ modulation. Combined, our data add another piece to the puzzle of the complex regulatory molecular networks controlling the general effects of tobacco smoke in CCs. This pilot study extends the until now modest number of studies simultaneously investigating the mtDNA-CN and TL pathways in the human CCs of smoking women. MDPI 2022-09-19 /pmc/articles/PMC9495883/ /pubmed/36139914 http://dx.doi.org/10.3390/antiox11091841 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Konstantinidou, Fani
Budani, Maria Cristina
Marconi, Guya Diletta
Gonnella, Francesca
Sarra, Annalina
Trubiani, Oriana
Stuppia, Liborio
Tiboni, Gian Mario
Gatta, Valentina
The Aftermath of Long-Term Cigarette Smoking on Telomere Length and Mitochondrial DNA Copy Number in Human Cumulus Cells Prior to In Vitro Fertilization—A Pilot Study
title The Aftermath of Long-Term Cigarette Smoking on Telomere Length and Mitochondrial DNA Copy Number in Human Cumulus Cells Prior to In Vitro Fertilization—A Pilot Study
title_full The Aftermath of Long-Term Cigarette Smoking on Telomere Length and Mitochondrial DNA Copy Number in Human Cumulus Cells Prior to In Vitro Fertilization—A Pilot Study
title_fullStr The Aftermath of Long-Term Cigarette Smoking on Telomere Length and Mitochondrial DNA Copy Number in Human Cumulus Cells Prior to In Vitro Fertilization—A Pilot Study
title_full_unstemmed The Aftermath of Long-Term Cigarette Smoking on Telomere Length and Mitochondrial DNA Copy Number in Human Cumulus Cells Prior to In Vitro Fertilization—A Pilot Study
title_short The Aftermath of Long-Term Cigarette Smoking on Telomere Length and Mitochondrial DNA Copy Number in Human Cumulus Cells Prior to In Vitro Fertilization—A Pilot Study
title_sort aftermath of long-term cigarette smoking on telomere length and mitochondrial dna copy number in human cumulus cells prior to in vitro fertilization—a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495883/
https://www.ncbi.nlm.nih.gov/pubmed/36139914
http://dx.doi.org/10.3390/antiox11091841
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