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A Ratiometric Organic Fluorescent Nanogel Thermometer for Highly Sensitive Temperature Sensing

Sensing temperature in biological systems is of great importance, as it is constructive to understanding various physiological and pathological processes. However, the realization of highly sensitive temperature sensing with organic fluorescent nanothermometers remains challenging. In this study, we...

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Detalles Bibliográficos
Autores principales: Wang, Chao, Zhao, Xianhao, Wu, Kaiyu, Lv, Shuyi, Zhu, Chunlei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496083/
https://www.ncbi.nlm.nih.gov/pubmed/36140087
http://dx.doi.org/10.3390/bios12090702
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author Wang, Chao
Zhao, Xianhao
Wu, Kaiyu
Lv, Shuyi
Zhu, Chunlei
author_facet Wang, Chao
Zhao, Xianhao
Wu, Kaiyu
Lv, Shuyi
Zhu, Chunlei
author_sort Wang, Chao
collection PubMed
description Sensing temperature in biological systems is of great importance, as it is constructive to understanding various physiological and pathological processes. However, the realization of highly sensitive temperature sensing with organic fluorescent nanothermometers remains challenging. In this study, we report a ratiometric fluorescent nanogel thermometer and study its application in the determination of bactericidal temperature. The nanogel is composed of a polarity-sensitive aggregation-induced emission luminogen with dual emissions, a thermoresponsive polymer with a phase transition function, and an ionic surface with net positive charges. During temperature-induced phase transition, the nanogel exhibits a reversible and sensitive spectral change between a red-emissive state and a blue-emissive state by responding to the hydrophilic-to-hydrophobic change in the local environment. The correlation between the emission intensity ratio of the two states and the external temperature is delicately established, and the maximum relative thermal sensitivities of the optimal nanogel are determined to be 128.42 and 68.39% °C(−1) in water and a simulated physiological environment, respectively. The nanogel is further applied to indicate the bactericidal temperature in both visual and ratiometric ways, holding great promise in the rapid prediction of photothermal antibacterial effects and other temperature-related biological events.
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spelling pubmed-94960832022-09-23 A Ratiometric Organic Fluorescent Nanogel Thermometer for Highly Sensitive Temperature Sensing Wang, Chao Zhao, Xianhao Wu, Kaiyu Lv, Shuyi Zhu, Chunlei Biosensors (Basel) Article Sensing temperature in biological systems is of great importance, as it is constructive to understanding various physiological and pathological processes. However, the realization of highly sensitive temperature sensing with organic fluorescent nanothermometers remains challenging. In this study, we report a ratiometric fluorescent nanogel thermometer and study its application in the determination of bactericidal temperature. The nanogel is composed of a polarity-sensitive aggregation-induced emission luminogen with dual emissions, a thermoresponsive polymer with a phase transition function, and an ionic surface with net positive charges. During temperature-induced phase transition, the nanogel exhibits a reversible and sensitive spectral change between a red-emissive state and a blue-emissive state by responding to the hydrophilic-to-hydrophobic change in the local environment. The correlation between the emission intensity ratio of the two states and the external temperature is delicately established, and the maximum relative thermal sensitivities of the optimal nanogel are determined to be 128.42 and 68.39% °C(−1) in water and a simulated physiological environment, respectively. The nanogel is further applied to indicate the bactericidal temperature in both visual and ratiometric ways, holding great promise in the rapid prediction of photothermal antibacterial effects and other temperature-related biological events. MDPI 2022-09-01 /pmc/articles/PMC9496083/ /pubmed/36140087 http://dx.doi.org/10.3390/bios12090702 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Chao
Zhao, Xianhao
Wu, Kaiyu
Lv, Shuyi
Zhu, Chunlei
A Ratiometric Organic Fluorescent Nanogel Thermometer for Highly Sensitive Temperature Sensing
title A Ratiometric Organic Fluorescent Nanogel Thermometer for Highly Sensitive Temperature Sensing
title_full A Ratiometric Organic Fluorescent Nanogel Thermometer for Highly Sensitive Temperature Sensing
title_fullStr A Ratiometric Organic Fluorescent Nanogel Thermometer for Highly Sensitive Temperature Sensing
title_full_unstemmed A Ratiometric Organic Fluorescent Nanogel Thermometer for Highly Sensitive Temperature Sensing
title_short A Ratiometric Organic Fluorescent Nanogel Thermometer for Highly Sensitive Temperature Sensing
title_sort ratiometric organic fluorescent nanogel thermometer for highly sensitive temperature sensing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496083/
https://www.ncbi.nlm.nih.gov/pubmed/36140087
http://dx.doi.org/10.3390/bios12090702
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