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Isolation of Hepatocytes from Liver Tissue by a Novel, Semi-Automated Perfusion Technology

Primary hepatocytes are a major tool in biomedical research. However, obtaining high yields of variable hepatocytes is technically challenging. Most protocols rely on the two-step collagenase perfusion protocol introduced by Per Ottar Seglen in 1976. In this procedure, the liver is perfused in situ...

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Detalles Bibliográficos
Autores principales:	Poggel, Carsten, Adams, Timo, Janzen, Ronald, Hofmann, Alexander, Hardt, Olaf, Roeb, Elke, Schröder, Sarah K., Tag, Carmen G., Roderfeld, Martin, Weiskirchen, Ralf
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496349/ https://www.ncbi.nlm.nih.gov/pubmed/36140299 http://dx.doi.org/10.3390/biomedicines10092198

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