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Metabolic Abnormalities Linked to Auditory Pathways in ApoE-Knockout HEI-OC1 Cells: A Transcription-Metabolism Co-Analysis

Lipid transporter protein apolipoprotein E (APOE) has contributed to functional studies of various organ functions. Animals with ApoE knockout (KO) have been used to study atherosclerosis and hyperlipidemia while an increasing number of researchers have recently focused on the association of ApoE wi...

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Detalles Bibliográficos
Autores principales: Ma, Lu, Wang, Hongshun, Yao, Jun, Wei, Qinjun, Cao, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496352/
https://www.ncbi.nlm.nih.gov/pubmed/36139057
http://dx.doi.org/10.3390/biom12091217
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author Ma, Lu
Wang, Hongshun
Yao, Jun
Wei, Qinjun
Cao, Xin
author_facet Ma, Lu
Wang, Hongshun
Yao, Jun
Wei, Qinjun
Cao, Xin
author_sort Ma, Lu
collection PubMed
description Lipid transporter protein apolipoprotein E (APOE) has contributed to functional studies of various organ functions. Animals with ApoE knockout (KO) have been used to study atherosclerosis and hyperlipidemia while an increasing number of researchers have recently focused on the association of ApoE with hearing loss. A study found that ApoE KO mice experience sensorineural hearing loss and hair cell loss, but the exact mechanism is unclear. To explore the potential relationship between ApoE and hearing loss, we used HEI-OC1 cells (House Ear Institute-Organ of Corti) with Corti apparatus properties to reveal cell changes after ApoE knockout by combined transcriptome and metabolomic analysis. We found that glutamate deficiency, caused by reduced expression of glutamine transporter proteins, was a key correlate of basal metabolism and that inadequate glutamate causes apoptosis by reducing the cells’ resistance to external damage. Our study provides a reference mechanism for hearing loss due to ApoE KO.
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spelling pubmed-94963522022-09-23 Metabolic Abnormalities Linked to Auditory Pathways in ApoE-Knockout HEI-OC1 Cells: A Transcription-Metabolism Co-Analysis Ma, Lu Wang, Hongshun Yao, Jun Wei, Qinjun Cao, Xin Biomolecules Article Lipid transporter protein apolipoprotein E (APOE) has contributed to functional studies of various organ functions. Animals with ApoE knockout (KO) have been used to study atherosclerosis and hyperlipidemia while an increasing number of researchers have recently focused on the association of ApoE with hearing loss. A study found that ApoE KO mice experience sensorineural hearing loss and hair cell loss, but the exact mechanism is unclear. To explore the potential relationship between ApoE and hearing loss, we used HEI-OC1 cells (House Ear Institute-Organ of Corti) with Corti apparatus properties to reveal cell changes after ApoE knockout by combined transcriptome and metabolomic analysis. We found that glutamate deficiency, caused by reduced expression of glutamine transporter proteins, was a key correlate of basal metabolism and that inadequate glutamate causes apoptosis by reducing the cells’ resistance to external damage. Our study provides a reference mechanism for hearing loss due to ApoE KO. MDPI 2022-09-01 /pmc/articles/PMC9496352/ /pubmed/36139057 http://dx.doi.org/10.3390/biom12091217 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Lu
Wang, Hongshun
Yao, Jun
Wei, Qinjun
Cao, Xin
Metabolic Abnormalities Linked to Auditory Pathways in ApoE-Knockout HEI-OC1 Cells: A Transcription-Metabolism Co-Analysis
title Metabolic Abnormalities Linked to Auditory Pathways in ApoE-Knockout HEI-OC1 Cells: A Transcription-Metabolism Co-Analysis
title_full Metabolic Abnormalities Linked to Auditory Pathways in ApoE-Knockout HEI-OC1 Cells: A Transcription-Metabolism Co-Analysis
title_fullStr Metabolic Abnormalities Linked to Auditory Pathways in ApoE-Knockout HEI-OC1 Cells: A Transcription-Metabolism Co-Analysis
title_full_unstemmed Metabolic Abnormalities Linked to Auditory Pathways in ApoE-Knockout HEI-OC1 Cells: A Transcription-Metabolism Co-Analysis
title_short Metabolic Abnormalities Linked to Auditory Pathways in ApoE-Knockout HEI-OC1 Cells: A Transcription-Metabolism Co-Analysis
title_sort metabolic abnormalities linked to auditory pathways in apoe-knockout hei-oc1 cells: a transcription-metabolism co-analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496352/
https://www.ncbi.nlm.nih.gov/pubmed/36139057
http://dx.doi.org/10.3390/biom12091217
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