Cargando…

Effects of RIPC on the Metabolome in Patients Undergoing Vascular Surgery: A Randomized Controlled Trial

Background: remote ischemic preconditioning (RIPC) is a phenomenon in which short episodes of ischemia are applied to distant organs to prepare target organs for more prolonged ischemia and to induce protection against ischemia-reperfusion injury. This study aims to evaluate whether preoperatively p...

Descripción completa

Detalles Bibliográficos
Autores principales: Eerik, Kadri, Kasepalu, Teele, Kuusik, Karl, Eha, Jaan, Vähi, Mare, Kilk, Kalle, Zilmer, Mihkel, Kals, Jaak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496371/
https://www.ncbi.nlm.nih.gov/pubmed/36139151
http://dx.doi.org/10.3390/biom12091312
_version_ 1784794252574720000
author Eerik, Kadri
Kasepalu, Teele
Kuusik, Karl
Eha, Jaan
Vähi, Mare
Kilk, Kalle
Zilmer, Mihkel
Kals, Jaak
author_facet Eerik, Kadri
Kasepalu, Teele
Kuusik, Karl
Eha, Jaan
Vähi, Mare
Kilk, Kalle
Zilmer, Mihkel
Kals, Jaak
author_sort Eerik, Kadri
collection PubMed
description Background: remote ischemic preconditioning (RIPC) is a phenomenon in which short episodes of ischemia are applied to distant organs to prepare target organs for more prolonged ischemia and to induce protection against ischemia-reperfusion injury. This study aims to evaluate whether preoperatively performed RIPC affects the metabolome and to assess whether metabolomic changes correlate with heart and kidney injury markers after vascular surgery. Methods: a randomized sham-controlled, double-blinded trial was conducted at Tartu University Hospital. Patients undergoing elective open vascular surgery were recruited and RIPC was applied before operation. Blood was collected preoperatively and 24 h postoperatively. The metabolome was analyzed using the AbsoluteIDQ p180 Kit. Results: final analysis included 45 patients from the RIPC group and 47 from the sham group. RIPC did not significantly alter metabolites 24 h postoperatively. There was positive correlation of change in the kynurenine/tryptophan ratio with change in hs-troponin T (r = 0.570, p < 0.001), NT-proBNP (r = 0.552, p < 0.001), cystatin C (r = 0.534, p < 0.001) and beta-2-microglobulin (r = 0.504, p < 0.001) only in the RIPC group. Conclusions: preoperative RIPC did not significantly affect the metabolome 24 h after vascular surgery. The positive linear correlation of kynurenine/tryptophan ratio with heart and kidney injury markers suggests that the kynurenine–tryptophan pathway can play a role in RIPC-associated cardio- and nephroprotective effects.
format Online
Article
Text
id pubmed-9496371
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94963712022-09-23 Effects of RIPC on the Metabolome in Patients Undergoing Vascular Surgery: A Randomized Controlled Trial Eerik, Kadri Kasepalu, Teele Kuusik, Karl Eha, Jaan Vähi, Mare Kilk, Kalle Zilmer, Mihkel Kals, Jaak Biomolecules Article Background: remote ischemic preconditioning (RIPC) is a phenomenon in which short episodes of ischemia are applied to distant organs to prepare target organs for more prolonged ischemia and to induce protection against ischemia-reperfusion injury. This study aims to evaluate whether preoperatively performed RIPC affects the metabolome and to assess whether metabolomic changes correlate with heart and kidney injury markers after vascular surgery. Methods: a randomized sham-controlled, double-blinded trial was conducted at Tartu University Hospital. Patients undergoing elective open vascular surgery were recruited and RIPC was applied before operation. Blood was collected preoperatively and 24 h postoperatively. The metabolome was analyzed using the AbsoluteIDQ p180 Kit. Results: final analysis included 45 patients from the RIPC group and 47 from the sham group. RIPC did not significantly alter metabolites 24 h postoperatively. There was positive correlation of change in the kynurenine/tryptophan ratio with change in hs-troponin T (r = 0.570, p < 0.001), NT-proBNP (r = 0.552, p < 0.001), cystatin C (r = 0.534, p < 0.001) and beta-2-microglobulin (r = 0.504, p < 0.001) only in the RIPC group. Conclusions: preoperative RIPC did not significantly affect the metabolome 24 h after vascular surgery. The positive linear correlation of kynurenine/tryptophan ratio with heart and kidney injury markers suggests that the kynurenine–tryptophan pathway can play a role in RIPC-associated cardio- and nephroprotective effects. MDPI 2022-09-16 /pmc/articles/PMC9496371/ /pubmed/36139151 http://dx.doi.org/10.3390/biom12091312 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Eerik, Kadri
Kasepalu, Teele
Kuusik, Karl
Eha, Jaan
Vähi, Mare
Kilk, Kalle
Zilmer, Mihkel
Kals, Jaak
Effects of RIPC on the Metabolome in Patients Undergoing Vascular Surgery: A Randomized Controlled Trial
title Effects of RIPC on the Metabolome in Patients Undergoing Vascular Surgery: A Randomized Controlled Trial
title_full Effects of RIPC on the Metabolome in Patients Undergoing Vascular Surgery: A Randomized Controlled Trial
title_fullStr Effects of RIPC on the Metabolome in Patients Undergoing Vascular Surgery: A Randomized Controlled Trial
title_full_unstemmed Effects of RIPC on the Metabolome in Patients Undergoing Vascular Surgery: A Randomized Controlled Trial
title_short Effects of RIPC on the Metabolome in Patients Undergoing Vascular Surgery: A Randomized Controlled Trial
title_sort effects of ripc on the metabolome in patients undergoing vascular surgery: a randomized controlled trial
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496371/
https://www.ncbi.nlm.nih.gov/pubmed/36139151
http://dx.doi.org/10.3390/biom12091312
work_keys_str_mv AT eerikkadri effectsofripconthemetabolomeinpatientsundergoingvascularsurgeryarandomizedcontrolledtrial
AT kasepaluteele effectsofripconthemetabolomeinpatientsundergoingvascularsurgeryarandomizedcontrolledtrial
AT kuusikkarl effectsofripconthemetabolomeinpatientsundergoingvascularsurgeryarandomizedcontrolledtrial
AT ehajaan effectsofripconthemetabolomeinpatientsundergoingvascularsurgeryarandomizedcontrolledtrial
AT vahimare effectsofripconthemetabolomeinpatientsundergoingvascularsurgeryarandomizedcontrolledtrial
AT kilkkalle effectsofripconthemetabolomeinpatientsundergoingvascularsurgeryarandomizedcontrolledtrial
AT zilmermihkel effectsofripconthemetabolomeinpatientsundergoingvascularsurgeryarandomizedcontrolledtrial
AT kalsjaak effectsofripconthemetabolomeinpatientsundergoingvascularsurgeryarandomizedcontrolledtrial