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Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration

Age-related macular degeneration (AMD) is an eye disease that can cause central vision loss, particularly in the elderly population. There are 2 classes of AMD, wet-type and dry-type. Wet-type involves excess angiogenesis around the macula, referred to as choroidal neovascularization (CNV). This can...

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Autores principales: Logan, Connor, Lyzogubov, Valeriy, Bora, Nalini, Bora, Puran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496372/
https://www.ncbi.nlm.nih.gov/pubmed/36139070
http://dx.doi.org/10.3390/biom12091232
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author Logan, Connor
Lyzogubov, Valeriy
Bora, Nalini
Bora, Puran
author_facet Logan, Connor
Lyzogubov, Valeriy
Bora, Nalini
Bora, Puran
author_sort Logan, Connor
collection PubMed
description Age-related macular degeneration (AMD) is an eye disease that can cause central vision loss, particularly in the elderly population. There are 2 classes of AMD, wet-type and dry-type. Wet-type involves excess angiogenesis around the macula, referred to as choroidal neovascularization (CNV). This can result in leaky vessels, often causing more severe vision loss than dry-type AMD. Adiponectin peptide 1 (APNp1) has been shown to slow the progression of CNV. Here, we used a mouse model and FITC-labeled APNp1 to determine if APNp1 could be delivered effectively as an eye drop. Our experiment revealed that topically applied FITC-APNp1 could reach the macula of the eye, which is crucial for treating wet-type AMD. We also tested delivery of APNp1 via injection of an adeno-associated virus (AAV) vector in a mouse model of CNV. AAV is a harmless virus easy to manipulate and is very often used for protein or peptide deliveries. Results revealed an increase in the expression of APNp1 in the retina and choroid over a 28-day period. Finally, we investigated the mechanism by which APNp1 affects CNV by examining the expression of adiponectin receptor 1 (AdipoR1) and proliferating cell nuclear antigen (PCNA) in the retinal and choroidal tissue of the mouse eyes. AdipoR1 and PCNA were overexpressed in these tissues in mice with laser-induced CNV compared to naïve mice. Based on our data shown here, we think it will enhance our understanding of APNp1 as a therapeutic agent for wet-type AMD and possible treatment alternatives that could be more beneficial for patients.
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spelling pubmed-94963722022-09-23 Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration Logan, Connor Lyzogubov, Valeriy Bora, Nalini Bora, Puran Biomolecules Article Age-related macular degeneration (AMD) is an eye disease that can cause central vision loss, particularly in the elderly population. There are 2 classes of AMD, wet-type and dry-type. Wet-type involves excess angiogenesis around the macula, referred to as choroidal neovascularization (CNV). This can result in leaky vessels, often causing more severe vision loss than dry-type AMD. Adiponectin peptide 1 (APNp1) has been shown to slow the progression of CNV. Here, we used a mouse model and FITC-labeled APNp1 to determine if APNp1 could be delivered effectively as an eye drop. Our experiment revealed that topically applied FITC-APNp1 could reach the macula of the eye, which is crucial for treating wet-type AMD. We also tested delivery of APNp1 via injection of an adeno-associated virus (AAV) vector in a mouse model of CNV. AAV is a harmless virus easy to manipulate and is very often used for protein or peptide deliveries. Results revealed an increase in the expression of APNp1 in the retina and choroid over a 28-day period. Finally, we investigated the mechanism by which APNp1 affects CNV by examining the expression of adiponectin receptor 1 (AdipoR1) and proliferating cell nuclear antigen (PCNA) in the retinal and choroidal tissue of the mouse eyes. AdipoR1 and PCNA were overexpressed in these tissues in mice with laser-induced CNV compared to naïve mice. Based on our data shown here, we think it will enhance our understanding of APNp1 as a therapeutic agent for wet-type AMD and possible treatment alternatives that could be more beneficial for patients. MDPI 2022-09-03 /pmc/articles/PMC9496372/ /pubmed/36139070 http://dx.doi.org/10.3390/biom12091232 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Logan, Connor
Lyzogubov, Valeriy
Bora, Nalini
Bora, Puran
Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration
title Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration
title_full Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration
title_fullStr Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration
title_full_unstemmed Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration
title_short Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration
title_sort role of adiponectin peptide i (apnp1) in age-related macular degeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496372/
https://www.ncbi.nlm.nih.gov/pubmed/36139070
http://dx.doi.org/10.3390/biom12091232
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