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Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration
Age-related macular degeneration (AMD) is an eye disease that can cause central vision loss, particularly in the elderly population. There are 2 classes of AMD, wet-type and dry-type. Wet-type involves excess angiogenesis around the macula, referred to as choroidal neovascularization (CNV). This can...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496372/ https://www.ncbi.nlm.nih.gov/pubmed/36139070 http://dx.doi.org/10.3390/biom12091232 |
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author | Logan, Connor Lyzogubov, Valeriy Bora, Nalini Bora, Puran |
author_facet | Logan, Connor Lyzogubov, Valeriy Bora, Nalini Bora, Puran |
author_sort | Logan, Connor |
collection | PubMed |
description | Age-related macular degeneration (AMD) is an eye disease that can cause central vision loss, particularly in the elderly population. There are 2 classes of AMD, wet-type and dry-type. Wet-type involves excess angiogenesis around the macula, referred to as choroidal neovascularization (CNV). This can result in leaky vessels, often causing more severe vision loss than dry-type AMD. Adiponectin peptide 1 (APNp1) has been shown to slow the progression of CNV. Here, we used a mouse model and FITC-labeled APNp1 to determine if APNp1 could be delivered effectively as an eye drop. Our experiment revealed that topically applied FITC-APNp1 could reach the macula of the eye, which is crucial for treating wet-type AMD. We also tested delivery of APNp1 via injection of an adeno-associated virus (AAV) vector in a mouse model of CNV. AAV is a harmless virus easy to manipulate and is very often used for protein or peptide deliveries. Results revealed an increase in the expression of APNp1 in the retina and choroid over a 28-day period. Finally, we investigated the mechanism by which APNp1 affects CNV by examining the expression of adiponectin receptor 1 (AdipoR1) and proliferating cell nuclear antigen (PCNA) in the retinal and choroidal tissue of the mouse eyes. AdipoR1 and PCNA were overexpressed in these tissues in mice with laser-induced CNV compared to naïve mice. Based on our data shown here, we think it will enhance our understanding of APNp1 as a therapeutic agent for wet-type AMD and possible treatment alternatives that could be more beneficial for patients. |
format | Online Article Text |
id | pubmed-9496372 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94963722022-09-23 Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration Logan, Connor Lyzogubov, Valeriy Bora, Nalini Bora, Puran Biomolecules Article Age-related macular degeneration (AMD) is an eye disease that can cause central vision loss, particularly in the elderly population. There are 2 classes of AMD, wet-type and dry-type. Wet-type involves excess angiogenesis around the macula, referred to as choroidal neovascularization (CNV). This can result in leaky vessels, often causing more severe vision loss than dry-type AMD. Adiponectin peptide 1 (APNp1) has been shown to slow the progression of CNV. Here, we used a mouse model and FITC-labeled APNp1 to determine if APNp1 could be delivered effectively as an eye drop. Our experiment revealed that topically applied FITC-APNp1 could reach the macula of the eye, which is crucial for treating wet-type AMD. We also tested delivery of APNp1 via injection of an adeno-associated virus (AAV) vector in a mouse model of CNV. AAV is a harmless virus easy to manipulate and is very often used for protein or peptide deliveries. Results revealed an increase in the expression of APNp1 in the retina and choroid over a 28-day period. Finally, we investigated the mechanism by which APNp1 affects CNV by examining the expression of adiponectin receptor 1 (AdipoR1) and proliferating cell nuclear antigen (PCNA) in the retinal and choroidal tissue of the mouse eyes. AdipoR1 and PCNA were overexpressed in these tissues in mice with laser-induced CNV compared to naïve mice. Based on our data shown here, we think it will enhance our understanding of APNp1 as a therapeutic agent for wet-type AMD and possible treatment alternatives that could be more beneficial for patients. MDPI 2022-09-03 /pmc/articles/PMC9496372/ /pubmed/36139070 http://dx.doi.org/10.3390/biom12091232 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Logan, Connor Lyzogubov, Valeriy Bora, Nalini Bora, Puran Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration |
title | Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration |
title_full | Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration |
title_fullStr | Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration |
title_full_unstemmed | Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration |
title_short | Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration |
title_sort | role of adiponectin peptide i (apnp1) in age-related macular degeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496372/ https://www.ncbi.nlm.nih.gov/pubmed/36139070 http://dx.doi.org/10.3390/biom12091232 |
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