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Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy
The rise in number of infections from multidrug-resistant (MDR) Gram-negative microbes has led to an increase in the use of a variety of ‘polymyxins’ such as colistin. Even though colistin is known to cause minor nephro- and neuro-toxicity, it is still considered as last resort antibiotic for treati...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496396/ https://www.ncbi.nlm.nih.gov/pubmed/36140134 http://dx.doi.org/10.3390/bios12090749 |
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author | Saikia, Dimple Jadhav, Priyanka Hole, Arti R. Krishna, Chilakapati Murali Singh, Surya P. |
author_facet | Saikia, Dimple Jadhav, Priyanka Hole, Arti R. Krishna, Chilakapati Murali Singh, Surya P. |
author_sort | Saikia, Dimple |
collection | PubMed |
description | The rise in number of infections from multidrug-resistant (MDR) Gram-negative microbes has led to an increase in the use of a variety of ‘polymyxins’ such as colistin. Even though colistin is known to cause minor nephro- and neuro-toxicity, it is still considered as last resort antibiotic for treating MDR infections. In this study, we have applied Raman spectroscopy to understand the differences among colistin sensitive and resistant bacterial strains at community level. We have successfully generated colistin resistant clones and verified the presence of resistance-causing MCR-1 plasmid. A unique spectral profile associated with specific drug concentration has been obtained. Successful delineation between resistant and sensitive cells has also been achieved via principal component analysis. Overall findings support the prospective utility of Raman spectroscopy in identifying anti-microbial resistance. |
format | Online Article Text |
id | pubmed-9496396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94963962022-09-23 Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy Saikia, Dimple Jadhav, Priyanka Hole, Arti R. Krishna, Chilakapati Murali Singh, Surya P. Biosensors (Basel) Article The rise in number of infections from multidrug-resistant (MDR) Gram-negative microbes has led to an increase in the use of a variety of ‘polymyxins’ such as colistin. Even though colistin is known to cause minor nephro- and neuro-toxicity, it is still considered as last resort antibiotic for treating MDR infections. In this study, we have applied Raman spectroscopy to understand the differences among colistin sensitive and resistant bacterial strains at community level. We have successfully generated colistin resistant clones and verified the presence of resistance-causing MCR-1 plasmid. A unique spectral profile associated with specific drug concentration has been obtained. Successful delineation between resistant and sensitive cells has also been achieved via principal component analysis. Overall findings support the prospective utility of Raman spectroscopy in identifying anti-microbial resistance. MDPI 2022-09-11 /pmc/articles/PMC9496396/ /pubmed/36140134 http://dx.doi.org/10.3390/bios12090749 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Saikia, Dimple Jadhav, Priyanka Hole, Arti R. Krishna, Chilakapati Murali Singh, Surya P. Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy |
title | Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy |
title_full | Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy |
title_fullStr | Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy |
title_full_unstemmed | Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy |
title_short | Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy |
title_sort | unraveling the secrets of colistin resistance with label-free raman spectroscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496396/ https://www.ncbi.nlm.nih.gov/pubmed/36140134 http://dx.doi.org/10.3390/bios12090749 |
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