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Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy

The rise in number of infections from multidrug-resistant (MDR) Gram-negative microbes has led to an increase in the use of a variety of ‘polymyxins’ such as colistin. Even though colistin is known to cause minor nephro- and neuro-toxicity, it is still considered as last resort antibiotic for treati...

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Autores principales: Saikia, Dimple, Jadhav, Priyanka, Hole, Arti R., Krishna, Chilakapati Murali, Singh, Surya P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496396/
https://www.ncbi.nlm.nih.gov/pubmed/36140134
http://dx.doi.org/10.3390/bios12090749
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author Saikia, Dimple
Jadhav, Priyanka
Hole, Arti R.
Krishna, Chilakapati Murali
Singh, Surya P.
author_facet Saikia, Dimple
Jadhav, Priyanka
Hole, Arti R.
Krishna, Chilakapati Murali
Singh, Surya P.
author_sort Saikia, Dimple
collection PubMed
description The rise in number of infections from multidrug-resistant (MDR) Gram-negative microbes has led to an increase in the use of a variety of ‘polymyxins’ such as colistin. Even though colistin is known to cause minor nephro- and neuro-toxicity, it is still considered as last resort antibiotic for treating MDR infections. In this study, we have applied Raman spectroscopy to understand the differences among colistin sensitive and resistant bacterial strains at community level. We have successfully generated colistin resistant clones and verified the presence of resistance-causing MCR-1 plasmid. A unique spectral profile associated with specific drug concentration has been obtained. Successful delineation between resistant and sensitive cells has also been achieved via principal component analysis. Overall findings support the prospective utility of Raman spectroscopy in identifying anti-microbial resistance.
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spelling pubmed-94963962022-09-23 Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy Saikia, Dimple Jadhav, Priyanka Hole, Arti R. Krishna, Chilakapati Murali Singh, Surya P. Biosensors (Basel) Article The rise in number of infections from multidrug-resistant (MDR) Gram-negative microbes has led to an increase in the use of a variety of ‘polymyxins’ such as colistin. Even though colistin is known to cause minor nephro- and neuro-toxicity, it is still considered as last resort antibiotic for treating MDR infections. In this study, we have applied Raman spectroscopy to understand the differences among colistin sensitive and resistant bacterial strains at community level. We have successfully generated colistin resistant clones and verified the presence of resistance-causing MCR-1 plasmid. A unique spectral profile associated with specific drug concentration has been obtained. Successful delineation between resistant and sensitive cells has also been achieved via principal component analysis. Overall findings support the prospective utility of Raman spectroscopy in identifying anti-microbial resistance. MDPI 2022-09-11 /pmc/articles/PMC9496396/ /pubmed/36140134 http://dx.doi.org/10.3390/bios12090749 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saikia, Dimple
Jadhav, Priyanka
Hole, Arti R.
Krishna, Chilakapati Murali
Singh, Surya P.
Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy
title Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy
title_full Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy
title_fullStr Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy
title_full_unstemmed Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy
title_short Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy
title_sort unraveling the secrets of colistin resistance with label-free raman spectroscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496396/
https://www.ncbi.nlm.nih.gov/pubmed/36140134
http://dx.doi.org/10.3390/bios12090749
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