An Immunochromatographic Assay for the Rapid and Qualitative Detection of Mercury in Rice

Mercury is a major pollutant in food crops. In this study, we synthesized an anti-mercury monoclonal antibody (mAb; IC(50) was 0.606 ng mL(−1)) with high sensitivity and specificity and different immunogens and coating antigens and developed an immuno-chromatographic assay (ICA) for the detection of...

Descripción completa

Detalles Bibliográficos
Autores principales: Lv, Shuai, Xu, Xinxin, Song, Shanshan, Xu, Liguang, Liu, Liqiang, Xu, Chuanlai, Kuang, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496535/
https://www.ncbi.nlm.nih.gov/pubmed/36140079
http://dx.doi.org/10.3390/bios12090694
Descripción
Sumario:Mercury is a major pollutant in food crops. In this study, we synthesized an anti-mercury monoclonal antibody (mAb; IC(50) was 0.606 ng mL(−1)) with high sensitivity and specificity and different immunogens and coating antigens and developed an immuno-chromatographic assay (ICA) for the detection of mercury in rice. The ICA strip had a visible detection limit of 20 ng g(−1) and a cut-off value of 500 ng g(−1) in rice. The performance of the ICA strip was consistent with that of ICP-MS and ic-ELISA. The recoveries of mercury in rice ranged from 94.5% to 113.7% with ic-ELISA and from 93.6% to 116.45% with ICP-MS. Qualitative analysis by ICA can be obtained with the naked eye. The ICA strip is an effective and practical method for the rapid and high-throughput determination of mercury in rice.

Ejemplares similares