An Immunochromatographic Assay for the Rapid and Qualitative Detection of Mercury in Rice

Mercury is a major pollutant in food crops. In this study, we synthesized an anti-mercury monoclonal antibody (mAb; IC(50) was 0.606 ng mL(−1)) with high sensitivity and specificity and different immunogens and coating antigens and developed an immuno-chromatographic assay (ICA) for the detection of...

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Autores principales: Lv, Shuai, Xu, Xinxin, Song, Shanshan, Xu, Liguang, Liu, Liqiang, Xu, Chuanlai, Kuang, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496535/
https://www.ncbi.nlm.nih.gov/pubmed/36140079
http://dx.doi.org/10.3390/bios12090694
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author Lv, Shuai
Xu, Xinxin
Song, Shanshan
Xu, Liguang
Liu, Liqiang
Xu, Chuanlai
Kuang, Hua
author_facet Lv, Shuai
Xu, Xinxin
Song, Shanshan
Xu, Liguang
Liu, Liqiang
Xu, Chuanlai
Kuang, Hua
author_sort Lv, Shuai
collection PubMed
description Mercury is a major pollutant in food crops. In this study, we synthesized an anti-mercury monoclonal antibody (mAb; IC(50) was 0.606 ng mL(−1)) with high sensitivity and specificity and different immunogens and coating antigens and developed an immuno-chromatographic assay (ICA) for the detection of mercury in rice. The ICA strip had a visible detection limit of 20 ng g(−1) and a cut-off value of 500 ng g(−1) in rice. The performance of the ICA strip was consistent with that of ICP-MS and ic-ELISA. The recoveries of mercury in rice ranged from 94.5% to 113.7% with ic-ELISA and from 93.6% to 116.45% with ICP-MS. Qualitative analysis by ICA can be obtained with the naked eye. The ICA strip is an effective and practical method for the rapid and high-throughput determination of mercury in rice.
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spelling pubmed-94965352022-09-23 An Immunochromatographic Assay for the Rapid and Qualitative Detection of Mercury in Rice Lv, Shuai Xu, Xinxin Song, Shanshan Xu, Liguang Liu, Liqiang Xu, Chuanlai Kuang, Hua Biosensors (Basel) Article Mercury is a major pollutant in food crops. In this study, we synthesized an anti-mercury monoclonal antibody (mAb; IC(50) was 0.606 ng mL(−1)) with high sensitivity and specificity and different immunogens and coating antigens and developed an immuno-chromatographic assay (ICA) for the detection of mercury in rice. The ICA strip had a visible detection limit of 20 ng g(−1) and a cut-off value of 500 ng g(−1) in rice. The performance of the ICA strip was consistent with that of ICP-MS and ic-ELISA. The recoveries of mercury in rice ranged from 94.5% to 113.7% with ic-ELISA and from 93.6% to 116.45% with ICP-MS. Qualitative analysis by ICA can be obtained with the naked eye. The ICA strip is an effective and practical method for the rapid and high-throughput determination of mercury in rice. MDPI 2022-08-28 /pmc/articles/PMC9496535/ /pubmed/36140079 http://dx.doi.org/10.3390/bios12090694 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lv, Shuai
Xu, Xinxin
Song, Shanshan
Xu, Liguang
Liu, Liqiang
Xu, Chuanlai
Kuang, Hua
An Immunochromatographic Assay for the Rapid and Qualitative Detection of Mercury in Rice
title An Immunochromatographic Assay for the Rapid and Qualitative Detection of Mercury in Rice
title_full An Immunochromatographic Assay for the Rapid and Qualitative Detection of Mercury in Rice
title_fullStr An Immunochromatographic Assay for the Rapid and Qualitative Detection of Mercury in Rice
title_full_unstemmed An Immunochromatographic Assay for the Rapid and Qualitative Detection of Mercury in Rice
title_short An Immunochromatographic Assay for the Rapid and Qualitative Detection of Mercury in Rice
title_sort immunochromatographic assay for the rapid and qualitative detection of mercury in rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496535/
https://www.ncbi.nlm.nih.gov/pubmed/36140079
http://dx.doi.org/10.3390/bios12090694
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