Cargando…

t(6)A and ms(2)t(6)A Modified Nucleosides in Serum and Urine as Strong Candidate Biomarkers of COVID-19 Infection and Severity

SARS-CoV-2 infection alters cellular RNA content. Cellular RNAs are chemically modified and eventually degraded, depositing modified nucleosides into extracellular fluids such as serum and urine. Here we searched for COVID-19-specific changes in modified nucleoside levels contained in serum and urin...

Descripción completa

Detalles Bibliográficos
Autores principales: Nagayoshi, Yu, Nishiguchi, Kayo, Yamamura, Ryosuke, Chujo, Takeshi, Oshiumi, Hiroyuki, Nagata, Hiroko, Kaneko, Hitomi, Yamamoto, Keiichi, Nakata, Hirotomo, Sakakida, Korin, Kunisawa, Akihiro, Adachi, Masataka, Kakizoe, Yutaka, Mizobe, Takanori, Kuratsu, Jun-ichi, Shimada, Shinya, Nakamori, Yasushi, Matsuoka, Masao, Mukoyama, Masashi, Wei, Fan-Yan, Tomizawa, Kazuhito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496545/
https://www.ncbi.nlm.nih.gov/pubmed/36139072
http://dx.doi.org/10.3390/biom12091233
Descripción
Sumario:SARS-CoV-2 infection alters cellular RNA content. Cellular RNAs are chemically modified and eventually degraded, depositing modified nucleosides into extracellular fluids such as serum and urine. Here we searched for COVID-19-specific changes in modified nucleoside levels contained in serum and urine of 308 COVID-19 patients using liquid chromatography-mass spectrometry (LC-MS). We found that two modified nucleosides, N(6)-threonylcarbamoyladenosine (t(6)A) and 2-methylthio-N(6)-threonylcarbamoyladenosine (ms(2)t(6)A), were elevated in serum and urine of COVID-19 patients. Moreover, these levels were associated with symptom severity and decreased upon recovery from COVID-19. In addition, the elevation of similarly modified nucleosides was observed regardless of COVID-19 variants. These findings illuminate specific modified RNA nucleosides in the extracellular fluids as biomarkers for COVID-19 infection and severity.