t(6)A and ms(2)t(6)A Modified Nucleosides in Serum and Urine as Strong Candidate Biomarkers of COVID-19 Infection and Severity

SARS-CoV-2 infection alters cellular RNA content. Cellular RNAs are chemically modified and eventually degraded, depositing modified nucleosides into extracellular fluids such as serum and urine. Here we searched for COVID-19-specific changes in modified nucleoside levels contained in serum and urin...

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Autores principales: Nagayoshi, Yu, Nishiguchi, Kayo, Yamamura, Ryosuke, Chujo, Takeshi, Oshiumi, Hiroyuki, Nagata, Hiroko, Kaneko, Hitomi, Yamamoto, Keiichi, Nakata, Hirotomo, Sakakida, Korin, Kunisawa, Akihiro, Adachi, Masataka, Kakizoe, Yutaka, Mizobe, Takanori, Kuratsu, Jun-ichi, Shimada, Shinya, Nakamori, Yasushi, Matsuoka, Masao, Mukoyama, Masashi, Wei, Fan-Yan, Tomizawa, Kazuhito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496545/
https://www.ncbi.nlm.nih.gov/pubmed/36139072
http://dx.doi.org/10.3390/biom12091233
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author Nagayoshi, Yu
Nishiguchi, Kayo
Yamamura, Ryosuke
Chujo, Takeshi
Oshiumi, Hiroyuki
Nagata, Hiroko
Kaneko, Hitomi
Yamamoto, Keiichi
Nakata, Hirotomo
Sakakida, Korin
Kunisawa, Akihiro
Adachi, Masataka
Kakizoe, Yutaka
Mizobe, Takanori
Kuratsu, Jun-ichi
Shimada, Shinya
Nakamori, Yasushi
Matsuoka, Masao
Mukoyama, Masashi
Wei, Fan-Yan
Tomizawa, Kazuhito
author_facet Nagayoshi, Yu
Nishiguchi, Kayo
Yamamura, Ryosuke
Chujo, Takeshi
Oshiumi, Hiroyuki
Nagata, Hiroko
Kaneko, Hitomi
Yamamoto, Keiichi
Nakata, Hirotomo
Sakakida, Korin
Kunisawa, Akihiro
Adachi, Masataka
Kakizoe, Yutaka
Mizobe, Takanori
Kuratsu, Jun-ichi
Shimada, Shinya
Nakamori, Yasushi
Matsuoka, Masao
Mukoyama, Masashi
Wei, Fan-Yan
Tomizawa, Kazuhito
author_sort Nagayoshi, Yu
collection PubMed
description SARS-CoV-2 infection alters cellular RNA content. Cellular RNAs are chemically modified and eventually degraded, depositing modified nucleosides into extracellular fluids such as serum and urine. Here we searched for COVID-19-specific changes in modified nucleoside levels contained in serum and urine of 308 COVID-19 patients using liquid chromatography-mass spectrometry (LC-MS). We found that two modified nucleosides, N(6)-threonylcarbamoyladenosine (t(6)A) and 2-methylthio-N(6)-threonylcarbamoyladenosine (ms(2)t(6)A), were elevated in serum and urine of COVID-19 patients. Moreover, these levels were associated with symptom severity and decreased upon recovery from COVID-19. In addition, the elevation of similarly modified nucleosides was observed regardless of COVID-19 variants. These findings illuminate specific modified RNA nucleosides in the extracellular fluids as biomarkers for COVID-19 infection and severity.
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spelling pubmed-94965452022-09-23 t(6)A and ms(2)t(6)A Modified Nucleosides in Serum and Urine as Strong Candidate Biomarkers of COVID-19 Infection and Severity Nagayoshi, Yu Nishiguchi, Kayo Yamamura, Ryosuke Chujo, Takeshi Oshiumi, Hiroyuki Nagata, Hiroko Kaneko, Hitomi Yamamoto, Keiichi Nakata, Hirotomo Sakakida, Korin Kunisawa, Akihiro Adachi, Masataka Kakizoe, Yutaka Mizobe, Takanori Kuratsu, Jun-ichi Shimada, Shinya Nakamori, Yasushi Matsuoka, Masao Mukoyama, Masashi Wei, Fan-Yan Tomizawa, Kazuhito Biomolecules Article SARS-CoV-2 infection alters cellular RNA content. Cellular RNAs are chemically modified and eventually degraded, depositing modified nucleosides into extracellular fluids such as serum and urine. Here we searched for COVID-19-specific changes in modified nucleoside levels contained in serum and urine of 308 COVID-19 patients using liquid chromatography-mass spectrometry (LC-MS). We found that two modified nucleosides, N(6)-threonylcarbamoyladenosine (t(6)A) and 2-methylthio-N(6)-threonylcarbamoyladenosine (ms(2)t(6)A), were elevated in serum and urine of COVID-19 patients. Moreover, these levels were associated with symptom severity and decreased upon recovery from COVID-19. In addition, the elevation of similarly modified nucleosides was observed regardless of COVID-19 variants. These findings illuminate specific modified RNA nucleosides in the extracellular fluids as biomarkers for COVID-19 infection and severity. MDPI 2022-09-03 /pmc/articles/PMC9496545/ /pubmed/36139072 http://dx.doi.org/10.3390/biom12091233 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nagayoshi, Yu
Nishiguchi, Kayo
Yamamura, Ryosuke
Chujo, Takeshi
Oshiumi, Hiroyuki
Nagata, Hiroko
Kaneko, Hitomi
Yamamoto, Keiichi
Nakata, Hirotomo
Sakakida, Korin
Kunisawa, Akihiro
Adachi, Masataka
Kakizoe, Yutaka
Mizobe, Takanori
Kuratsu, Jun-ichi
Shimada, Shinya
Nakamori, Yasushi
Matsuoka, Masao
Mukoyama, Masashi
Wei, Fan-Yan
Tomizawa, Kazuhito
t(6)A and ms(2)t(6)A Modified Nucleosides in Serum and Urine as Strong Candidate Biomarkers of COVID-19 Infection and Severity
title t(6)A and ms(2)t(6)A Modified Nucleosides in Serum and Urine as Strong Candidate Biomarkers of COVID-19 Infection and Severity
title_full t(6)A and ms(2)t(6)A Modified Nucleosides in Serum and Urine as Strong Candidate Biomarkers of COVID-19 Infection and Severity
title_fullStr t(6)A and ms(2)t(6)A Modified Nucleosides in Serum and Urine as Strong Candidate Biomarkers of COVID-19 Infection and Severity
title_full_unstemmed t(6)A and ms(2)t(6)A Modified Nucleosides in Serum and Urine as Strong Candidate Biomarkers of COVID-19 Infection and Severity
title_short t(6)A and ms(2)t(6)A Modified Nucleosides in Serum and Urine as Strong Candidate Biomarkers of COVID-19 Infection and Severity
title_sort t(6)a and ms(2)t(6)a modified nucleosides in serum and urine as strong candidate biomarkers of covid-19 infection and severity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496545/
https://www.ncbi.nlm.nih.gov/pubmed/36139072
http://dx.doi.org/10.3390/biom12091233
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