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Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene

A sensitive and selective electrochemical deoxyribonucleic acid (DNA) biosensor was developed for the determination of a osteosarcoma-related survivin gene by using celestine blue (CB) as a label-free hybridization indicator. The proposed strategy adopted a facile and low-cost working electrode with...

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Detalles Bibliográficos
Autores principales: Chen, Yao, Zhong, Yu, Ye, Ji-Xing, Lei, Yun, Liu, Ai-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496566/
https://www.ncbi.nlm.nih.gov/pubmed/36140132
http://dx.doi.org/10.3390/bios12090747
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author Chen, Yao
Zhong, Yu
Ye, Ji-Xing
Lei, Yun
Liu, Ai-Lin
author_facet Chen, Yao
Zhong, Yu
Ye, Ji-Xing
Lei, Yun
Liu, Ai-Lin
author_sort Chen, Yao
collection PubMed
description A sensitive and selective electrochemical deoxyribonucleic acid (DNA) biosensor was developed for the determination of a osteosarcoma-related survivin gene by using celestine blue (CB) as a label-free hybridization indicator. The proposed strategy adopted a facile and low-cost working electrode with no need for other substances for electrode or DNA functionalization. The interaction mode between CB and DNA was studied by electrochemical and spectroscopic approaches, illustrating that the possible mode was intercalation with a binding number of 2 and a binding constant β of 10(12.87). Moreover, the label-free electrochemical DNA biosensor exhibited a good linear relationship toward the target gene in a range from 1.00 nM to 50.00 nM with a detection limit as low as 0.046 nM using 3σ estimating system. This facile and low-cost electrochemical method realized the rapid detection and accurate quantification of the target sequence in complicated serum samples, endowing its promising potential in the diagnosis and monitoring of genetic diseases.
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spelling pubmed-94965662022-09-23 Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene Chen, Yao Zhong, Yu Ye, Ji-Xing Lei, Yun Liu, Ai-Lin Biosensors (Basel) Article A sensitive and selective electrochemical deoxyribonucleic acid (DNA) biosensor was developed for the determination of a osteosarcoma-related survivin gene by using celestine blue (CB) as a label-free hybridization indicator. The proposed strategy adopted a facile and low-cost working electrode with no need for other substances for electrode or DNA functionalization. The interaction mode between CB and DNA was studied by electrochemical and spectroscopic approaches, illustrating that the possible mode was intercalation with a binding number of 2 and a binding constant β of 10(12.87). Moreover, the label-free electrochemical DNA biosensor exhibited a good linear relationship toward the target gene in a range from 1.00 nM to 50.00 nM with a detection limit as low as 0.046 nM using 3σ estimating system. This facile and low-cost electrochemical method realized the rapid detection and accurate quantification of the target sequence in complicated serum samples, endowing its promising potential in the diagnosis and monitoring of genetic diseases. MDPI 2022-09-09 /pmc/articles/PMC9496566/ /pubmed/36140132 http://dx.doi.org/10.3390/bios12090747 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Yao
Zhong, Yu
Ye, Ji-Xing
Lei, Yun
Liu, Ai-Lin
Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene
title Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene
title_full Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene
title_fullStr Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene
title_full_unstemmed Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene
title_short Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene
title_sort facile label-free electrochemical dna biosensor for detection of osteosarcoma-related survivin gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496566/
https://www.ncbi.nlm.nih.gov/pubmed/36140132
http://dx.doi.org/10.3390/bios12090747
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