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Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes

Covalent organic frameworks (COFs) are gaining growing interest owing to their various structures and versatility. Since their specific physical–chemical characteristics endow them great usage potentiality in biosensing, we herein have synthesized spherical COFs with regular shape and good dispersio...

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Autores principales: Lin, Yuxin, Nie, Beibei, Qu, Xinyu, Wang, Minghui, Yang, Jie, Li, Genxi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496618/
https://www.ncbi.nlm.nih.gov/pubmed/36140089
http://dx.doi.org/10.3390/bios12090704
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author Lin, Yuxin
Nie, Beibei
Qu, Xinyu
Wang, Minghui
Yang, Jie
Li, Genxi
author_facet Lin, Yuxin
Nie, Beibei
Qu, Xinyu
Wang, Minghui
Yang, Jie
Li, Genxi
author_sort Lin, Yuxin
collection PubMed
description Covalent organic frameworks (COFs) are gaining growing interest owing to their various structures and versatility. Since their specific physical–chemical characteristics endow them great usage potentiality in biosensing, we herein have synthesized spherical COFs with regular shape and good dispersion, which are further used for the design of a novel nanoprobe by modifying Histostar on the surface of the COFs. Moreover, we have applied a nanoprobe for the fabrication of an electrochemical biosensor to detect exosomes. Since Histostar is a special polymer, conjugated with many secondary antibodies (IgG), and HRP can increase the availability of HRP at the antigenic site, the biosensor can have a strong signal amplification ability. Meanwhile, since COFs with high porosity can be loaded with a huge amount of Histostar, the sensitivity of the biosensor can be further improved. With such a design, the proposed biosensor can achieve a low exosomes detection limit of 318 particles/µL, and a wide linear detection range from 10(3) particles/µL to 10(8) particles/µL. So, this work may offer a promising platform for the ultrasensitive detection of exosomes.
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spelling pubmed-94966182022-09-23 Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes Lin, Yuxin Nie, Beibei Qu, Xinyu Wang, Minghui Yang, Jie Li, Genxi Biosensors (Basel) Communication Covalent organic frameworks (COFs) are gaining growing interest owing to their various structures and versatility. Since their specific physical–chemical characteristics endow them great usage potentiality in biosensing, we herein have synthesized spherical COFs with regular shape and good dispersion, which are further used for the design of a novel nanoprobe by modifying Histostar on the surface of the COFs. Moreover, we have applied a nanoprobe for the fabrication of an electrochemical biosensor to detect exosomes. Since Histostar is a special polymer, conjugated with many secondary antibodies (IgG), and HRP can increase the availability of HRP at the antigenic site, the biosensor can have a strong signal amplification ability. Meanwhile, since COFs with high porosity can be loaded with a huge amount of Histostar, the sensitivity of the biosensor can be further improved. With such a design, the proposed biosensor can achieve a low exosomes detection limit of 318 particles/µL, and a wide linear detection range from 10(3) particles/µL to 10(8) particles/µL. So, this work may offer a promising platform for the ultrasensitive detection of exosomes. MDPI 2022-09-01 /pmc/articles/PMC9496618/ /pubmed/36140089 http://dx.doi.org/10.3390/bios12090704 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Lin, Yuxin
Nie, Beibei
Qu, Xinyu
Wang, Minghui
Yang, Jie
Li, Genxi
Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes
title Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes
title_full Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes
title_fullStr Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes
title_full_unstemmed Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes
title_short Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes
title_sort histostar-functionalized covalent organic framework for electrochemical detection of exosomes
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9496618/
https://www.ncbi.nlm.nih.gov/pubmed/36140089
http://dx.doi.org/10.3390/bios12090704
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