CTRP1 Knockout Attenuates Tumor Progression in A549 and HCT116 Cancer Cells

SIMPLE SUMMARY: CTRP1 belongs to the C1q and TNF-related protein family, and we generated CTRP1 knockout cells to examine the role of CTRP1 in tumor progression. CTRP1 knockout attenuates cell growth, invasion and tumor growth in mice, suggesting that CTRP1 expression promotes tumor progression. ABSTRACT: C1q and TNF-related 1 (C1QTNF1/CTRP1) is an adiponectin-associated protein belonging to the C1q/TNF-related protein family. Recent studies have shown that the C1q and TNF-related protein (CTRP) family is involved in cancer progression; however, the specific role of CTRP1 in tumor progression has not yet been elucidated. To examine the role of CTRP1 in tumor progression, we generated CTRP1 knockout A549 and HCT116 cell lines, which reduced the expression levels of nuclear factor (NF)-κB-dependent and metastasis-promoting transcripts. We demonstrated that CTRP1 knockout inhibited the cell proliferation and invasion and tumor growth. Finally, database analysis showed that CTRP1 expression was upregulated in metastatic cancers and elevated levels of CTRP1 were associated with poor prognosis. These results suggest that CTRP1 expression contributes to NF-κB signaling and promotes tumor progression.