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MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency

Premature ovarian insufficiency (POI) is a typical disorder of amenorrhea that lasts for a minimum of four months in women < 40 years old and is typically characterized by reduced estrogen levels and elevated serum concentrations of follicle-stimulating hormone. We collected urine samples from tw...

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Autores principales: Umair, Zobia, Baek, Mi-Ock, Song, Jisue, An, Seona, Chon, Seung Joo, Yoon, Mee-Sup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9497098/
https://www.ncbi.nlm.nih.gov/pubmed/36139370
http://dx.doi.org/10.3390/cells11182797
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author Umair, Zobia
Baek, Mi-Ock
Song, Jisue
An, Seona
Chon, Seung Joo
Yoon, Mee-Sup
author_facet Umair, Zobia
Baek, Mi-Ock
Song, Jisue
An, Seona
Chon, Seung Joo
Yoon, Mee-Sup
author_sort Umair, Zobia
collection PubMed
description Premature ovarian insufficiency (POI) is a typical disorder of amenorrhea that lasts for a minimum of four months in women < 40 years old and is typically characterized by reduced estrogen levels and elevated serum concentrations of follicle-stimulating hormone. We collected urine samples from two participant cohorts from Gil Hospital of Gachon University (Incheon, Korea): a sequencing cohort of 19 participants (seven patients with POI (POI patients without Turner syndrome), seven patients with Turner syndrome (POI patients with Turner syndrome), and five control individuals (age-matched controls with confirmed ovarian sufficiency)) and a validation cohort of 46 participants (15 patients with POI, 11 patients with Turner syndrome, and 20 control individuals). Among differentially expressed miRNAs, hsa-miR-4516 was significantly upregulated in patients with POI in both cohorts, independent of the presence of Turner syndrome. Moreover, the upregulation of miR-4516 was confirmed in the ovary—but not in the uterus—of a cyclophosphamide and busulfan-induced POI mouse model. This was accompanied by a decrease in STAT3 protein level, a predicted target of miR-4516, via miRTarBase2020. Our study provides compelling evidence that miR-4516 is highly expressed in patients with POI and POI mouse models, suggesting that miR-4516 is a diagnostic marker of POI.
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spelling pubmed-94970982022-09-23 MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency Umair, Zobia Baek, Mi-Ock Song, Jisue An, Seona Chon, Seung Joo Yoon, Mee-Sup Cells Article Premature ovarian insufficiency (POI) is a typical disorder of amenorrhea that lasts for a minimum of four months in women < 40 years old and is typically characterized by reduced estrogen levels and elevated serum concentrations of follicle-stimulating hormone. We collected urine samples from two participant cohorts from Gil Hospital of Gachon University (Incheon, Korea): a sequencing cohort of 19 participants (seven patients with POI (POI patients without Turner syndrome), seven patients with Turner syndrome (POI patients with Turner syndrome), and five control individuals (age-matched controls with confirmed ovarian sufficiency)) and a validation cohort of 46 participants (15 patients with POI, 11 patients with Turner syndrome, and 20 control individuals). Among differentially expressed miRNAs, hsa-miR-4516 was significantly upregulated in patients with POI in both cohorts, independent of the presence of Turner syndrome. Moreover, the upregulation of miR-4516 was confirmed in the ovary—but not in the uterus—of a cyclophosphamide and busulfan-induced POI mouse model. This was accompanied by a decrease in STAT3 protein level, a predicted target of miR-4516, via miRTarBase2020. Our study provides compelling evidence that miR-4516 is highly expressed in patients with POI and POI mouse models, suggesting that miR-4516 is a diagnostic marker of POI. MDPI 2022-09-07 /pmc/articles/PMC9497098/ /pubmed/36139370 http://dx.doi.org/10.3390/cells11182797 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Umair, Zobia
Baek, Mi-Ock
Song, Jisue
An, Seona
Chon, Seung Joo
Yoon, Mee-Sup
MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency
title MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency
title_full MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency
title_fullStr MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency
title_full_unstemmed MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency
title_short MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency
title_sort microrna-4516 in urinary exosomes as a biomarker of premature ovarian insufficiency
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9497098/
https://www.ncbi.nlm.nih.gov/pubmed/36139370
http://dx.doi.org/10.3390/cells11182797
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