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A Comprehensive 2D-LC/MS/MS Profile of the Normal Human Urinary Metabolome
Profiling bodily fluids is crucial for monitoring and discovering metabolic markers of disease. In this study, a comprehensive analysis approach based on 1D-LC-MS/MS and 2D-LC-MS/MS was applied to profile normal human urine metabolites from 348 children and 315 adults. A total of 2357 metabolites we...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9497905/ https://www.ncbi.nlm.nih.gov/pubmed/36140585 http://dx.doi.org/10.3390/diagnostics12092184 |
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author | Xu, Jiyu Zheng, Shuxin Li, Mimi Liu, Xiaoyan Sun, Haidan Guo, Zhengguang Wei, Jing Jia, Lulu Sun, Wei |
author_facet | Xu, Jiyu Zheng, Shuxin Li, Mimi Liu, Xiaoyan Sun, Haidan Guo, Zhengguang Wei, Jing Jia, Lulu Sun, Wei |
author_sort | Xu, Jiyu |
collection | PubMed |
description | Profiling bodily fluids is crucial for monitoring and discovering metabolic markers of disease. In this study, a comprehensive analysis approach based on 1D-LC-MS/MS and 2D-LC-MS/MS was applied to profile normal human urine metabolites from 348 children and 315 adults. A total of 2357 metabolites were identified, including 1831 endogenous metabolites and 526 exogenous ones. In total, 1005 metabolites were identified in urine for the first time. The urinary metabolites were mainly involved in amino acid metabolism, small molecule biochemistry, lipid metabolism and cellular compromise. The comparison of adult’s and children’s urine metabolomes showed adults urine had more metabolites involved in immune response than children’s, but the function of binding of melatonin, which belongs to the endocrine system, showed a higher expression in children. The urine metabolites detected by the 1D-LC-MS/MS method were mainly related to amino acid metabolism and lipid metabolism, and the 2D-LC-MS/MS method not only explored metabolites from 1D-LC-MS/MS but also metabolites related to cell signaling, cell function and maintenance, etc. Our analysis comprehensively profiled and functionally annotated the metabolome of normal human urine, which would benefit the application of urinary metabolome to clinical research. |
format | Online Article Text |
id | pubmed-9497905 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94979052022-09-23 A Comprehensive 2D-LC/MS/MS Profile of the Normal Human Urinary Metabolome Xu, Jiyu Zheng, Shuxin Li, Mimi Liu, Xiaoyan Sun, Haidan Guo, Zhengguang Wei, Jing Jia, Lulu Sun, Wei Diagnostics (Basel) Article Profiling bodily fluids is crucial for monitoring and discovering metabolic markers of disease. In this study, a comprehensive analysis approach based on 1D-LC-MS/MS and 2D-LC-MS/MS was applied to profile normal human urine metabolites from 348 children and 315 adults. A total of 2357 metabolites were identified, including 1831 endogenous metabolites and 526 exogenous ones. In total, 1005 metabolites were identified in urine for the first time. The urinary metabolites were mainly involved in amino acid metabolism, small molecule biochemistry, lipid metabolism and cellular compromise. The comparison of adult’s and children’s urine metabolomes showed adults urine had more metabolites involved in immune response than children’s, but the function of binding of melatonin, which belongs to the endocrine system, showed a higher expression in children. The urine metabolites detected by the 1D-LC-MS/MS method were mainly related to amino acid metabolism and lipid metabolism, and the 2D-LC-MS/MS method not only explored metabolites from 1D-LC-MS/MS but also metabolites related to cell signaling, cell function and maintenance, etc. Our analysis comprehensively profiled and functionally annotated the metabolome of normal human urine, which would benefit the application of urinary metabolome to clinical research. MDPI 2022-09-09 /pmc/articles/PMC9497905/ /pubmed/36140585 http://dx.doi.org/10.3390/diagnostics12092184 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xu, Jiyu Zheng, Shuxin Li, Mimi Liu, Xiaoyan Sun, Haidan Guo, Zhengguang Wei, Jing Jia, Lulu Sun, Wei A Comprehensive 2D-LC/MS/MS Profile of the Normal Human Urinary Metabolome |
title | A Comprehensive 2D-LC/MS/MS Profile of the Normal Human Urinary Metabolome |
title_full | A Comprehensive 2D-LC/MS/MS Profile of the Normal Human Urinary Metabolome |
title_fullStr | A Comprehensive 2D-LC/MS/MS Profile of the Normal Human Urinary Metabolome |
title_full_unstemmed | A Comprehensive 2D-LC/MS/MS Profile of the Normal Human Urinary Metabolome |
title_short | A Comprehensive 2D-LC/MS/MS Profile of the Normal Human Urinary Metabolome |
title_sort | comprehensive 2d-lc/ms/ms profile of the normal human urinary metabolome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9497905/ https://www.ncbi.nlm.nih.gov/pubmed/36140585 http://dx.doi.org/10.3390/diagnostics12092184 |
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