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Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases

Transcription factor PAX8, expressed during embryonic kidney development, has been previously detected in various kidney tumors. In order to investigate expression of PAX8 transcription factor in acute kidney injury (AKI) and chronic kidney diseases (CKD), immunohistochemical analysis was performed....

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Autores principales: Zivotic, Maja, Dundjerovic, Dusko, Naumovic, Radomir, Kovacevic, Sanjin, Ivanov, Milan, Karanovic, Danijela, Nikolic, Gorana, Markovic-Lipkovski, Jasmina, Radojevic Skodric, Sanja, Nesovic Ostojic, Jelena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9497907/
https://www.ncbi.nlm.nih.gov/pubmed/36140438
http://dx.doi.org/10.3390/diagnostics12092036
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author Zivotic, Maja
Dundjerovic, Dusko
Naumovic, Radomir
Kovacevic, Sanjin
Ivanov, Milan
Karanovic, Danijela
Nikolic, Gorana
Markovic-Lipkovski, Jasmina
Radojevic Skodric, Sanja
Nesovic Ostojic, Jelena
author_facet Zivotic, Maja
Dundjerovic, Dusko
Naumovic, Radomir
Kovacevic, Sanjin
Ivanov, Milan
Karanovic, Danijela
Nikolic, Gorana
Markovic-Lipkovski, Jasmina
Radojevic Skodric, Sanja
Nesovic Ostojic, Jelena
author_sort Zivotic, Maja
collection PubMed
description Transcription factor PAX8, expressed during embryonic kidney development, has been previously detected in various kidney tumors. In order to investigate expression of PAX8 transcription factor in acute kidney injury (AKI) and chronic kidney diseases (CKD), immunohistochemical analysis was performed. Presence, location and extent of PAX8 expression were analyzed among 31 human kidney samples of AKI (25 autopsy cases, 5 kidney biopsies with unknown etiology and 1 AKI with confirmed myoglobin cast nephropathy), as well as in animals with induced postischemic AKI. Additionally, expression pattern was analyzed in 20 kidney biopsy samples of CKD. Our study demonstrates that various kidney diseases with chronic disease course that results in the formation of tubular atrophy and interstitial fibrosis, lead to PAX8 expression in the nuclei of proximal tubules. Furthermore, patients with PAX8 detected within the damaged proximal tubuli would be carefully monitored, since deterioration in kidney function was observed during follow-up. We also showed that myoglobin provoked acute kidney injury followed with large extent of renal damage, was associated with strong nuclear expression of PAX8 in proximal tubular cells. These results were supported and followed by data obtained in experimental model of induced postischemic acute kidney injury. Considering these findings, we can assume that PAX8 protein might be involved in regeneration process and recovery after acute kidney injury. Thus, accordingly, all investigation concerning PAX8 immunolabeling should be performed on biopsy samples of the living individuals.
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spelling pubmed-94979072022-09-23 Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases Zivotic, Maja Dundjerovic, Dusko Naumovic, Radomir Kovacevic, Sanjin Ivanov, Milan Karanovic, Danijela Nikolic, Gorana Markovic-Lipkovski, Jasmina Radojevic Skodric, Sanja Nesovic Ostojic, Jelena Diagnostics (Basel) Article Transcription factor PAX8, expressed during embryonic kidney development, has been previously detected in various kidney tumors. In order to investigate expression of PAX8 transcription factor in acute kidney injury (AKI) and chronic kidney diseases (CKD), immunohistochemical analysis was performed. Presence, location and extent of PAX8 expression were analyzed among 31 human kidney samples of AKI (25 autopsy cases, 5 kidney biopsies with unknown etiology and 1 AKI with confirmed myoglobin cast nephropathy), as well as in animals with induced postischemic AKI. Additionally, expression pattern was analyzed in 20 kidney biopsy samples of CKD. Our study demonstrates that various kidney diseases with chronic disease course that results in the formation of tubular atrophy and interstitial fibrosis, lead to PAX8 expression in the nuclei of proximal tubules. Furthermore, patients with PAX8 detected within the damaged proximal tubuli would be carefully monitored, since deterioration in kidney function was observed during follow-up. We also showed that myoglobin provoked acute kidney injury followed with large extent of renal damage, was associated with strong nuclear expression of PAX8 in proximal tubular cells. These results were supported and followed by data obtained in experimental model of induced postischemic acute kidney injury. Considering these findings, we can assume that PAX8 protein might be involved in regeneration process and recovery after acute kidney injury. Thus, accordingly, all investigation concerning PAX8 immunolabeling should be performed on biopsy samples of the living individuals. MDPI 2022-08-23 /pmc/articles/PMC9497907/ /pubmed/36140438 http://dx.doi.org/10.3390/diagnostics12092036 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zivotic, Maja
Dundjerovic, Dusko
Naumovic, Radomir
Kovacevic, Sanjin
Ivanov, Milan
Karanovic, Danijela
Nikolic, Gorana
Markovic-Lipkovski, Jasmina
Radojevic Skodric, Sanja
Nesovic Ostojic, Jelena
Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases
title Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases
title_full Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases
title_fullStr Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases
title_full_unstemmed Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases
title_short Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases
title_sort clinicopathological relevance of pax8 expression patterns in acute kidney injury and chronic kidney diseases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9497907/
https://www.ncbi.nlm.nih.gov/pubmed/36140438
http://dx.doi.org/10.3390/diagnostics12092036
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