Molecular Characterization by Whole-Genome Sequencing of Clinical and Environmental Serratia marcescens Strains Isolated during an Outbreak in a Neonatal Intensive Care Unit (NICU)

The whole-genome sequencing (WGS) of eighteen S. marcescens clinical strains isolated from 18 newborns hospitalized in the Neonatal Intensive Care Unit (NICU) at Pescara Public Hospital, Italy, was compared with that of S. marcescens isolated from cradles surfaces in the same ward. The identical ant...

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Autores principales: Piccirilli, Alessandra, Cherubini, Sabrina, Brisdelli, Fabrizia, Fazii, Paolo, Stanziale, Andrea, Di Valerio, Susanna, Chiavaroli, Valentina, Principe, Luigi, Perilli, Mariagrazia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498040/
https://www.ncbi.nlm.nih.gov/pubmed/36140580
http://dx.doi.org/10.3390/diagnostics12092180
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author Piccirilli, Alessandra
Cherubini, Sabrina
Brisdelli, Fabrizia
Fazii, Paolo
Stanziale, Andrea
Di Valerio, Susanna
Chiavaroli, Valentina
Principe, Luigi
Perilli, Mariagrazia
author_facet Piccirilli, Alessandra
Cherubini, Sabrina
Brisdelli, Fabrizia
Fazii, Paolo
Stanziale, Andrea
Di Valerio, Susanna
Chiavaroli, Valentina
Principe, Luigi
Perilli, Mariagrazia
author_sort Piccirilli, Alessandra
collection PubMed
description The whole-genome sequencing (WGS) of eighteen S. marcescens clinical strains isolated from 18 newborns hospitalized in the Neonatal Intensive Care Unit (NICU) at Pescara Public Hospital, Italy, was compared with that of S. marcescens isolated from cradles surfaces in the same ward. The identical antibiotic resistance genes (ARGs) and virulence factors were found in both clinical and environmental S. marcescens strains. The aac(6′)-Ic, tetA(41), bla(SRT-3), adeFGH, rsmA, and PBP3 (D350N) genes were identified in all strains. The SRT-3 enzyme, which exhibited 10 amino acid substitutions with respect to SST-1, the constitutive AmpC β-lactamase in S. marcescens, was partially purified and tested against some β-lactams. It showed a good activity against cefazolin. Both clinical and environmental S. marcescens strains exhibited susceptibility to all antibiotics tested, with the exception of amoxicillin/clavulanate.
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spelling pubmed-94980402022-09-23 Molecular Characterization by Whole-Genome Sequencing of Clinical and Environmental Serratia marcescens Strains Isolated during an Outbreak in a Neonatal Intensive Care Unit (NICU) Piccirilli, Alessandra Cherubini, Sabrina Brisdelli, Fabrizia Fazii, Paolo Stanziale, Andrea Di Valerio, Susanna Chiavaroli, Valentina Principe, Luigi Perilli, Mariagrazia Diagnostics (Basel) Communication The whole-genome sequencing (WGS) of eighteen S. marcescens clinical strains isolated from 18 newborns hospitalized in the Neonatal Intensive Care Unit (NICU) at Pescara Public Hospital, Italy, was compared with that of S. marcescens isolated from cradles surfaces in the same ward. The identical antibiotic resistance genes (ARGs) and virulence factors were found in both clinical and environmental S. marcescens strains. The aac(6′)-Ic, tetA(41), bla(SRT-3), adeFGH, rsmA, and PBP3 (D350N) genes were identified in all strains. The SRT-3 enzyme, which exhibited 10 amino acid substitutions with respect to SST-1, the constitutive AmpC β-lactamase in S. marcescens, was partially purified and tested against some β-lactams. It showed a good activity against cefazolin. Both clinical and environmental S. marcescens strains exhibited susceptibility to all antibiotics tested, with the exception of amoxicillin/clavulanate. MDPI 2022-09-09 /pmc/articles/PMC9498040/ /pubmed/36140580 http://dx.doi.org/10.3390/diagnostics12092180 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Piccirilli, Alessandra
Cherubini, Sabrina
Brisdelli, Fabrizia
Fazii, Paolo
Stanziale, Andrea
Di Valerio, Susanna
Chiavaroli, Valentina
Principe, Luigi
Perilli, Mariagrazia
Molecular Characterization by Whole-Genome Sequencing of Clinical and Environmental Serratia marcescens Strains Isolated during an Outbreak in a Neonatal Intensive Care Unit (NICU)
title Molecular Characterization by Whole-Genome Sequencing of Clinical and Environmental Serratia marcescens Strains Isolated during an Outbreak in a Neonatal Intensive Care Unit (NICU)
title_full Molecular Characterization by Whole-Genome Sequencing of Clinical and Environmental Serratia marcescens Strains Isolated during an Outbreak in a Neonatal Intensive Care Unit (NICU)
title_fullStr Molecular Characterization by Whole-Genome Sequencing of Clinical and Environmental Serratia marcescens Strains Isolated during an Outbreak in a Neonatal Intensive Care Unit (NICU)
title_full_unstemmed Molecular Characterization by Whole-Genome Sequencing of Clinical and Environmental Serratia marcescens Strains Isolated during an Outbreak in a Neonatal Intensive Care Unit (NICU)
title_short Molecular Characterization by Whole-Genome Sequencing of Clinical and Environmental Serratia marcescens Strains Isolated during an Outbreak in a Neonatal Intensive Care Unit (NICU)
title_sort molecular characterization by whole-genome sequencing of clinical and environmental serratia marcescens strains isolated during an outbreak in a neonatal intensive care unit (nicu)
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498040/
https://www.ncbi.nlm.nih.gov/pubmed/36140580
http://dx.doi.org/10.3390/diagnostics12092180
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