Cargando…

LsARF3 mediates thermally induced bolting through promoting the expression of LsCO in lettuce (Lactuca sativa L.)

Lettuce (Lactuca sativa L.) is a leafy vegetable whose edible organs usually are leaf or stems, and thus high-temperature induced bolting followed by flower initiation is an undesirable trait in lettuce production. However, the molecular mechanism that controls lettuce bolting and flowering upon the...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Yunfeng, Zhu, Jiaqi, Feng, Yixuan, Li, Zhenfeng, Ren, Zheng, Liu, Ning, Liu, Chaojie, Hao, Jinghong, Han, Yingyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498183/
https://www.ncbi.nlm.nih.gov/pubmed/36160965
http://dx.doi.org/10.3389/fpls.2022.958833
_version_ 1784794694059819008
author Li, Yunfeng
Zhu, Jiaqi
Feng, Yixuan
Li, Zhenfeng
Ren, Zheng
Liu, Ning
Liu, Chaojie
Hao, Jinghong
Han, Yingyan
author_facet Li, Yunfeng
Zhu, Jiaqi
Feng, Yixuan
Li, Zhenfeng
Ren, Zheng
Liu, Ning
Liu, Chaojie
Hao, Jinghong
Han, Yingyan
author_sort Li, Yunfeng
collection PubMed
description Lettuce (Lactuca sativa L.) is a leafy vegetable whose edible organs usually are leaf or stems, and thus high-temperature induced bolting followed by flower initiation is an undesirable trait in lettuce production. However, the molecular mechanism that controls lettuce bolting and flowering upon thermal treatments is largely unknown. Here, we identified a Lettuce auxin response factor 3 (LsARF3), the expression of which was enhanced by heat and auxin treatments. Interestingly, LsARF3 is preferentially expressed in stem apex, suggesting it might be associated with lettuce bolting. Transgenic lettuce overexpressing LsARF3 displayed early bolting and flowering, whereas knockout of LsARF3 dramatically delayed bolting and flowering in lettuce under normal or high temperature conditions. Furthermore, Exogenous application of IAA failed to rescue the late-bolting and -flowering phenotype of lsarf3 mutants. Several floral integrator genes including LsCO, LsFT, and LsLFY were co-expressed with LsARF3 in the overexpression and knockout lettuce plants. Yeast one-hybrid (Y1H) experiments suggested that LsARF3 could physically interact with the LsCO promoter, which was further confirmed by a dual luciferase assay in tobacco leaves. The results indicated that LsARF3 might directly modulate the expression of LsCO in lettuce. Therefore, these results demonstrate that LsARF3 could promote lettuce bolting in response to the high temperature by directly or indirectly activating the expression of floral genes such as LsCO, which provides new insights into lettuce bolting in the context of ARFs signaling and heat response.
format Online
Article
Text
id pubmed-9498183
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-94981832022-09-23 LsARF3 mediates thermally induced bolting through promoting the expression of LsCO in lettuce (Lactuca sativa L.) Li, Yunfeng Zhu, Jiaqi Feng, Yixuan Li, Zhenfeng Ren, Zheng Liu, Ning Liu, Chaojie Hao, Jinghong Han, Yingyan Front Plant Sci Plant Science Lettuce (Lactuca sativa L.) is a leafy vegetable whose edible organs usually are leaf or stems, and thus high-temperature induced bolting followed by flower initiation is an undesirable trait in lettuce production. However, the molecular mechanism that controls lettuce bolting and flowering upon thermal treatments is largely unknown. Here, we identified a Lettuce auxin response factor 3 (LsARF3), the expression of which was enhanced by heat and auxin treatments. Interestingly, LsARF3 is preferentially expressed in stem apex, suggesting it might be associated with lettuce bolting. Transgenic lettuce overexpressing LsARF3 displayed early bolting and flowering, whereas knockout of LsARF3 dramatically delayed bolting and flowering in lettuce under normal or high temperature conditions. Furthermore, Exogenous application of IAA failed to rescue the late-bolting and -flowering phenotype of lsarf3 mutants. Several floral integrator genes including LsCO, LsFT, and LsLFY were co-expressed with LsARF3 in the overexpression and knockout lettuce plants. Yeast one-hybrid (Y1H) experiments suggested that LsARF3 could physically interact with the LsCO promoter, which was further confirmed by a dual luciferase assay in tobacco leaves. The results indicated that LsARF3 might directly modulate the expression of LsCO in lettuce. Therefore, these results demonstrate that LsARF3 could promote lettuce bolting in response to the high temperature by directly or indirectly activating the expression of floral genes such as LsCO, which provides new insights into lettuce bolting in the context of ARFs signaling and heat response. Frontiers Media S.A. 2022-09-08 /pmc/articles/PMC9498183/ /pubmed/36160965 http://dx.doi.org/10.3389/fpls.2022.958833 Text en Copyright © 2022 Li, Zhu, Feng, Li, Ren, Liu, Liu, Hao and Han. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Li, Yunfeng
Zhu, Jiaqi
Feng, Yixuan
Li, Zhenfeng
Ren, Zheng
Liu, Ning
Liu, Chaojie
Hao, Jinghong
Han, Yingyan
LsARF3 mediates thermally induced bolting through promoting the expression of LsCO in lettuce (Lactuca sativa L.)
title LsARF3 mediates thermally induced bolting through promoting the expression of LsCO in lettuce (Lactuca sativa L.)
title_full LsARF3 mediates thermally induced bolting through promoting the expression of LsCO in lettuce (Lactuca sativa L.)
title_fullStr LsARF3 mediates thermally induced bolting through promoting the expression of LsCO in lettuce (Lactuca sativa L.)
title_full_unstemmed LsARF3 mediates thermally induced bolting through promoting the expression of LsCO in lettuce (Lactuca sativa L.)
title_short LsARF3 mediates thermally induced bolting through promoting the expression of LsCO in lettuce (Lactuca sativa L.)
title_sort lsarf3 mediates thermally induced bolting through promoting the expression of lsco in lettuce (lactuca sativa l.)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498183/
https://www.ncbi.nlm.nih.gov/pubmed/36160965
http://dx.doi.org/10.3389/fpls.2022.958833
work_keys_str_mv AT liyunfeng lsarf3mediatesthermallyinducedboltingthroughpromotingtheexpressionoflscoinlettucelactucasatival
AT zhujiaqi lsarf3mediatesthermallyinducedboltingthroughpromotingtheexpressionoflscoinlettucelactucasatival
AT fengyixuan lsarf3mediatesthermallyinducedboltingthroughpromotingtheexpressionoflscoinlettucelactucasatival
AT lizhenfeng lsarf3mediatesthermallyinducedboltingthroughpromotingtheexpressionoflscoinlettucelactucasatival
AT renzheng lsarf3mediatesthermallyinducedboltingthroughpromotingtheexpressionoflscoinlettucelactucasatival
AT liuning lsarf3mediatesthermallyinducedboltingthroughpromotingtheexpressionoflscoinlettucelactucasatival
AT liuchaojie lsarf3mediatesthermallyinducedboltingthroughpromotingtheexpressionoflscoinlettucelactucasatival
AT haojinghong lsarf3mediatesthermallyinducedboltingthroughpromotingtheexpressionoflscoinlettucelactucasatival
AT hanyingyan lsarf3mediatesthermallyinducedboltingthroughpromotingtheexpressionoflscoinlettucelactucasatival