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Expression and Prognostic Value of Chromobox Family Proteins in Esophageal Cancer
Background: Esophageal cancer (EC) is one of the most common human malignant tumors worldwide. Chromobox (CBX) family proteins are significant components of epigenetic regulatory complexes. It is reported that CBXs play critical roles in the oncogenesis and development of various tumors. Nonetheless...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498422/ https://www.ncbi.nlm.nih.gov/pubmed/36140750 http://dx.doi.org/10.3390/genes13091582 |
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author | Liu, Jin Shen, Haixiang Chen, Xiangliu Ding, Yongfeng Wang, Haiyong Xu, Nong Teng, Lisong |
author_facet | Liu, Jin Shen, Haixiang Chen, Xiangliu Ding, Yongfeng Wang, Haiyong Xu, Nong Teng, Lisong |
author_sort | Liu, Jin |
collection | PubMed |
description | Background: Esophageal cancer (EC) is one of the most common human malignant tumors worldwide. Chromobox (CBX) family proteins are significant components of epigenetic regulatory complexes. It is reported that CBXs play critical roles in the oncogenesis and development of various tumors. Nonetheless, their functions and specific roles in EC remain vague and obscure. Methods and Materials: We used multiple bioinformatics tools, including Oncomine, Gene Expression Profiling Interactive Analysis 2 (GEPIA2), UALCAN, Kaplan–Meier plotter, cBioPortal, Metascape, TIMER2 and TISIDB, to investigate the expression profile, gene alterations and prognostic roles of CBX family proteins, as well as their association with clinicopathologic parameters, immune cells and immune regulators. In addition, RT-qPCR, Western blot, CCK8, colony formation, wound healing and transwell assays were performed to investigate the biological functions of CBX3 in EC cells. Results: CBX3 and CBX5 were overexpressed in EC compared to normal tissues. Survival analysis revealed that high expression of CBX1 predicted worse disease-free survival (DFS) in EC patients. Functionally, CBXs might participate in mismatch repair, spliceosome, cell cycle, the Fanconi anemia pathway, tight junction, the mRNA surveillance pathway and the Hippo signaling pathway in EC development. Furthermore, CBXs were related to distinct immune cells infiltration and immune regulators. Additionally, depletion of CBX3 inhibited the proliferation, migration and invasion abilities of EC cells. Conclusions: Our study comprehensively investigated the expression pattern, prognostic value, and gene alterations of CBXs in EC, as well as their relationships with clinicopathologic variables, immune cells infiltration and immune regulators. These results suggested that CBX family proteins, especially CBX3, might be potential biomarkers in the progression of EC. |
format | Online Article Text |
id | pubmed-9498422 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94984222022-09-23 Expression and Prognostic Value of Chromobox Family Proteins in Esophageal Cancer Liu, Jin Shen, Haixiang Chen, Xiangliu Ding, Yongfeng Wang, Haiyong Xu, Nong Teng, Lisong Genes (Basel) Article Background: Esophageal cancer (EC) is one of the most common human malignant tumors worldwide. Chromobox (CBX) family proteins are significant components of epigenetic regulatory complexes. It is reported that CBXs play critical roles in the oncogenesis and development of various tumors. Nonetheless, their functions and specific roles in EC remain vague and obscure. Methods and Materials: We used multiple bioinformatics tools, including Oncomine, Gene Expression Profiling Interactive Analysis 2 (GEPIA2), UALCAN, Kaplan–Meier plotter, cBioPortal, Metascape, TIMER2 and TISIDB, to investigate the expression profile, gene alterations and prognostic roles of CBX family proteins, as well as their association with clinicopathologic parameters, immune cells and immune regulators. In addition, RT-qPCR, Western blot, CCK8, colony formation, wound healing and transwell assays were performed to investigate the biological functions of CBX3 in EC cells. Results: CBX3 and CBX5 were overexpressed in EC compared to normal tissues. Survival analysis revealed that high expression of CBX1 predicted worse disease-free survival (DFS) in EC patients. Functionally, CBXs might participate in mismatch repair, spliceosome, cell cycle, the Fanconi anemia pathway, tight junction, the mRNA surveillance pathway and the Hippo signaling pathway in EC development. Furthermore, CBXs were related to distinct immune cells infiltration and immune regulators. Additionally, depletion of CBX3 inhibited the proliferation, migration and invasion abilities of EC cells. Conclusions: Our study comprehensively investigated the expression pattern, prognostic value, and gene alterations of CBXs in EC, as well as their relationships with clinicopathologic variables, immune cells infiltration and immune regulators. These results suggested that CBX family proteins, especially CBX3, might be potential biomarkers in the progression of EC. MDPI 2022-09-03 /pmc/articles/PMC9498422/ /pubmed/36140750 http://dx.doi.org/10.3390/genes13091582 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Jin Shen, Haixiang Chen, Xiangliu Ding, Yongfeng Wang, Haiyong Xu, Nong Teng, Lisong Expression and Prognostic Value of Chromobox Family Proteins in Esophageal Cancer |
title | Expression and Prognostic Value of Chromobox Family Proteins in Esophageal Cancer |
title_full | Expression and Prognostic Value of Chromobox Family Proteins in Esophageal Cancer |
title_fullStr | Expression and Prognostic Value of Chromobox Family Proteins in Esophageal Cancer |
title_full_unstemmed | Expression and Prognostic Value of Chromobox Family Proteins in Esophageal Cancer |
title_short | Expression and Prognostic Value of Chromobox Family Proteins in Esophageal Cancer |
title_sort | expression and prognostic value of chromobox family proteins in esophageal cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498422/ https://www.ncbi.nlm.nih.gov/pubmed/36140750 http://dx.doi.org/10.3390/genes13091582 |
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