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Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level

Salmonella Enteritidis (S. Enteritidis) can adapt to sublethal sodium hypochlorite conditions, which subsequently triggers stress resistance mechanisms in this pathogen. Hence, the current work aimed to reveal the underlying stress adaptation mechanisms in S. Enteritidis by phenotypic, proteomic, an...

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Autores principales: Li, Danhong, He, Shoukui, Dong, Rui, Cui, Yan, Shi, Xianming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498478/
https://www.ncbi.nlm.nih.gov/pubmed/36141039
http://dx.doi.org/10.3390/foods11182912
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author Li, Danhong
He, Shoukui
Dong, Rui
Cui, Yan
Shi, Xianming
author_facet Li, Danhong
He, Shoukui
Dong, Rui
Cui, Yan
Shi, Xianming
author_sort Li, Danhong
collection PubMed
description Salmonella Enteritidis (S. Enteritidis) can adapt to sublethal sodium hypochlorite conditions, which subsequently triggers stress resistance mechanisms in this pathogen. Hence, the current work aimed to reveal the underlying stress adaptation mechanisms in S. Enteritidis by phenotypic, proteomic, and physiological analyses. It was found that 130 ppm sodium hypochlorite resulted in a moderate inhibitory effect on bacterial growth and an increased accumulation of intracellular reactive oxygen species. In response to this sublethal treatment, a total of 492 proteins in S. Enteritidis showed significant differential abundance (p < 0.05; fold change >2.0 or <0.5), including 225 more abundant proteins and 267 less abundant proteins, as revealed by the tandem-mass-tags-based quantitative proteomics technology. Functional characterization further revealed that proteins related to flagellar assembly, two-component system, and phosphotransferase system were in less abundance, while those associated with ABC transporters were generally in more abundance. Specifically, the repression of flagellar-assembly-related proteins led to diminished swimming motility, which served as a potential energy conservation strategy. Moreover, altered abundance of lipid-metabolism-related proteins resulted in reduced cell membrane fluidity, which provided a survival advantage to S. Enteritidis. Taken together, these results indicate that S. Enteritidis employs multiple adaptation pathways to cope with sodium hypochlorite stress.
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spelling pubmed-94984782022-09-23 Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level Li, Danhong He, Shoukui Dong, Rui Cui, Yan Shi, Xianming Foods Article Salmonella Enteritidis (S. Enteritidis) can adapt to sublethal sodium hypochlorite conditions, which subsequently triggers stress resistance mechanisms in this pathogen. Hence, the current work aimed to reveal the underlying stress adaptation mechanisms in S. Enteritidis by phenotypic, proteomic, and physiological analyses. It was found that 130 ppm sodium hypochlorite resulted in a moderate inhibitory effect on bacterial growth and an increased accumulation of intracellular reactive oxygen species. In response to this sublethal treatment, a total of 492 proteins in S. Enteritidis showed significant differential abundance (p < 0.05; fold change >2.0 or <0.5), including 225 more abundant proteins and 267 less abundant proteins, as revealed by the tandem-mass-tags-based quantitative proteomics technology. Functional characterization further revealed that proteins related to flagellar assembly, two-component system, and phosphotransferase system were in less abundance, while those associated with ABC transporters were generally in more abundance. Specifically, the repression of flagellar-assembly-related proteins led to diminished swimming motility, which served as a potential energy conservation strategy. Moreover, altered abundance of lipid-metabolism-related proteins resulted in reduced cell membrane fluidity, which provided a survival advantage to S. Enteritidis. Taken together, these results indicate that S. Enteritidis employs multiple adaptation pathways to cope with sodium hypochlorite stress. MDPI 2022-09-19 /pmc/articles/PMC9498478/ /pubmed/36141039 http://dx.doi.org/10.3390/foods11182912 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Danhong
He, Shoukui
Dong, Rui
Cui, Yan
Shi, Xianming
Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level
title Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level
title_full Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level
title_fullStr Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level
title_full_unstemmed Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level
title_short Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level
title_sort stress response mechanisms of salmonella enteritidis to sodium hypochlorite at the proteomic level
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498478/
https://www.ncbi.nlm.nih.gov/pubmed/36141039
http://dx.doi.org/10.3390/foods11182912
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