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Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level
Salmonella Enteritidis (S. Enteritidis) can adapt to sublethal sodium hypochlorite conditions, which subsequently triggers stress resistance mechanisms in this pathogen. Hence, the current work aimed to reveal the underlying stress adaptation mechanisms in S. Enteritidis by phenotypic, proteomic, an...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498478/ https://www.ncbi.nlm.nih.gov/pubmed/36141039 http://dx.doi.org/10.3390/foods11182912 |
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author | Li, Danhong He, Shoukui Dong, Rui Cui, Yan Shi, Xianming |
author_facet | Li, Danhong He, Shoukui Dong, Rui Cui, Yan Shi, Xianming |
author_sort | Li, Danhong |
collection | PubMed |
description | Salmonella Enteritidis (S. Enteritidis) can adapt to sublethal sodium hypochlorite conditions, which subsequently triggers stress resistance mechanisms in this pathogen. Hence, the current work aimed to reveal the underlying stress adaptation mechanisms in S. Enteritidis by phenotypic, proteomic, and physiological analyses. It was found that 130 ppm sodium hypochlorite resulted in a moderate inhibitory effect on bacterial growth and an increased accumulation of intracellular reactive oxygen species. In response to this sublethal treatment, a total of 492 proteins in S. Enteritidis showed significant differential abundance (p < 0.05; fold change >2.0 or <0.5), including 225 more abundant proteins and 267 less abundant proteins, as revealed by the tandem-mass-tags-based quantitative proteomics technology. Functional characterization further revealed that proteins related to flagellar assembly, two-component system, and phosphotransferase system were in less abundance, while those associated with ABC transporters were generally in more abundance. Specifically, the repression of flagellar-assembly-related proteins led to diminished swimming motility, which served as a potential energy conservation strategy. Moreover, altered abundance of lipid-metabolism-related proteins resulted in reduced cell membrane fluidity, which provided a survival advantage to S. Enteritidis. Taken together, these results indicate that S. Enteritidis employs multiple adaptation pathways to cope with sodium hypochlorite stress. |
format | Online Article Text |
id | pubmed-9498478 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94984782022-09-23 Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level Li, Danhong He, Shoukui Dong, Rui Cui, Yan Shi, Xianming Foods Article Salmonella Enteritidis (S. Enteritidis) can adapt to sublethal sodium hypochlorite conditions, which subsequently triggers stress resistance mechanisms in this pathogen. Hence, the current work aimed to reveal the underlying stress adaptation mechanisms in S. Enteritidis by phenotypic, proteomic, and physiological analyses. It was found that 130 ppm sodium hypochlorite resulted in a moderate inhibitory effect on bacterial growth and an increased accumulation of intracellular reactive oxygen species. In response to this sublethal treatment, a total of 492 proteins in S. Enteritidis showed significant differential abundance (p < 0.05; fold change >2.0 or <0.5), including 225 more abundant proteins and 267 less abundant proteins, as revealed by the tandem-mass-tags-based quantitative proteomics technology. Functional characterization further revealed that proteins related to flagellar assembly, two-component system, and phosphotransferase system were in less abundance, while those associated with ABC transporters were generally in more abundance. Specifically, the repression of flagellar-assembly-related proteins led to diminished swimming motility, which served as a potential energy conservation strategy. Moreover, altered abundance of lipid-metabolism-related proteins resulted in reduced cell membrane fluidity, which provided a survival advantage to S. Enteritidis. Taken together, these results indicate that S. Enteritidis employs multiple adaptation pathways to cope with sodium hypochlorite stress. MDPI 2022-09-19 /pmc/articles/PMC9498478/ /pubmed/36141039 http://dx.doi.org/10.3390/foods11182912 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Danhong He, Shoukui Dong, Rui Cui, Yan Shi, Xianming Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level |
title | Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level |
title_full | Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level |
title_fullStr | Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level |
title_full_unstemmed | Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level |
title_short | Stress Response Mechanisms of Salmonella Enteritidis to Sodium Hypochlorite at the Proteomic Level |
title_sort | stress response mechanisms of salmonella enteritidis to sodium hypochlorite at the proteomic level |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498478/ https://www.ncbi.nlm.nih.gov/pubmed/36141039 http://dx.doi.org/10.3390/foods11182912 |
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