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Comparative Transcriptome Analyses of Different Rheum officinale Tissues Reveal Differentially Expressed Genes Associated with Anthraquinone, Catechin, and Gallic Acid Biosynthesis

Rheum officinale Baill. is an important traditional Chinese medicinal herb, its dried roots and rhizomes being widely utilized to cure diverse diseases. However, previous studies mainly focused on the active compounds and their pharmacological effects, and the molecular mechanism underlying the bios...

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Autores principales: Zhou, Lipan, Sun, Jiangyan, Zhang, Tianyi, Tang, Yadi, Liu, Jie, Gao, Chenxi, Zhai, Yunyan, Guo, Yanbing, Feng, Li, Zhang, Xinxin, Zhou, Tao, Wang, Xumei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498579/
https://www.ncbi.nlm.nih.gov/pubmed/36140760
http://dx.doi.org/10.3390/genes13091592
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author Zhou, Lipan
Sun, Jiangyan
Zhang, Tianyi
Tang, Yadi
Liu, Jie
Gao, Chenxi
Zhai, Yunyan
Guo, Yanbing
Feng, Li
Zhang, Xinxin
Zhou, Tao
Wang, Xumei
author_facet Zhou, Lipan
Sun, Jiangyan
Zhang, Tianyi
Tang, Yadi
Liu, Jie
Gao, Chenxi
Zhai, Yunyan
Guo, Yanbing
Feng, Li
Zhang, Xinxin
Zhou, Tao
Wang, Xumei
author_sort Zhou, Lipan
collection PubMed
description Rheum officinale Baill. is an important traditional Chinese medicinal herb, its dried roots and rhizomes being widely utilized to cure diverse diseases. However, previous studies mainly focused on the active compounds and their pharmacological effects, and the molecular mechanism underlying the biosynthesis of these ingredients in R. officinale is still elusive. Here, we performed comparative transcriptome analyses to elucidate the differentially expressed genes (DEGs) in the root, stem, and leaf of R. officinale. A total of 236,031 unigenes with N50 of 769 bp was generated, 136,329 (57.76%) of which were annotated. A total of 5884 DEGs was identified after the comparative analyses of different tissues; 175 and 126 key enzyme genes with tissue-specific expression were found in the anthraquinone, catechin/gallic acid biosynthetic pathway, respectively, and some of these key enzyme genes were verified by qRT-PCR. The phylogeny of the PKS III family in Polygonaceae indicated that probably only PL_741 PKSIII1, PL_11549 PKSIII5, and PL_101745 PKSIII6 encoded PKSIII in the polyketide pathway. These results will shed light on the molecular basis of the tissue-specific accumulation and regulation of secondary metabolites in R. officinale, and lay a foundation for the future genetic diversity, molecular assisted breeding, and germplasm resource improvement of this essential medicinal plant.
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spelling pubmed-94985792022-09-23 Comparative Transcriptome Analyses of Different Rheum officinale Tissues Reveal Differentially Expressed Genes Associated with Anthraquinone, Catechin, and Gallic Acid Biosynthesis Zhou, Lipan Sun, Jiangyan Zhang, Tianyi Tang, Yadi Liu, Jie Gao, Chenxi Zhai, Yunyan Guo, Yanbing Feng, Li Zhang, Xinxin Zhou, Tao Wang, Xumei Genes (Basel) Article Rheum officinale Baill. is an important traditional Chinese medicinal herb, its dried roots and rhizomes being widely utilized to cure diverse diseases. However, previous studies mainly focused on the active compounds and their pharmacological effects, and the molecular mechanism underlying the biosynthesis of these ingredients in R. officinale is still elusive. Here, we performed comparative transcriptome analyses to elucidate the differentially expressed genes (DEGs) in the root, stem, and leaf of R. officinale. A total of 236,031 unigenes with N50 of 769 bp was generated, 136,329 (57.76%) of which were annotated. A total of 5884 DEGs was identified after the comparative analyses of different tissues; 175 and 126 key enzyme genes with tissue-specific expression were found in the anthraquinone, catechin/gallic acid biosynthetic pathway, respectively, and some of these key enzyme genes were verified by qRT-PCR. The phylogeny of the PKS III family in Polygonaceae indicated that probably only PL_741 PKSIII1, PL_11549 PKSIII5, and PL_101745 PKSIII6 encoded PKSIII in the polyketide pathway. These results will shed light on the molecular basis of the tissue-specific accumulation and regulation of secondary metabolites in R. officinale, and lay a foundation for the future genetic diversity, molecular assisted breeding, and germplasm resource improvement of this essential medicinal plant. MDPI 2022-09-05 /pmc/articles/PMC9498579/ /pubmed/36140760 http://dx.doi.org/10.3390/genes13091592 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Lipan
Sun, Jiangyan
Zhang, Tianyi
Tang, Yadi
Liu, Jie
Gao, Chenxi
Zhai, Yunyan
Guo, Yanbing
Feng, Li
Zhang, Xinxin
Zhou, Tao
Wang, Xumei
Comparative Transcriptome Analyses of Different Rheum officinale Tissues Reveal Differentially Expressed Genes Associated with Anthraquinone, Catechin, and Gallic Acid Biosynthesis
title Comparative Transcriptome Analyses of Different Rheum officinale Tissues Reveal Differentially Expressed Genes Associated with Anthraquinone, Catechin, and Gallic Acid Biosynthesis
title_full Comparative Transcriptome Analyses of Different Rheum officinale Tissues Reveal Differentially Expressed Genes Associated with Anthraquinone, Catechin, and Gallic Acid Biosynthesis
title_fullStr Comparative Transcriptome Analyses of Different Rheum officinale Tissues Reveal Differentially Expressed Genes Associated with Anthraquinone, Catechin, and Gallic Acid Biosynthesis
title_full_unstemmed Comparative Transcriptome Analyses of Different Rheum officinale Tissues Reveal Differentially Expressed Genes Associated with Anthraquinone, Catechin, and Gallic Acid Biosynthesis
title_short Comparative Transcriptome Analyses of Different Rheum officinale Tissues Reveal Differentially Expressed Genes Associated with Anthraquinone, Catechin, and Gallic Acid Biosynthesis
title_sort comparative transcriptome analyses of different rheum officinale tissues reveal differentially expressed genes associated with anthraquinone, catechin, and gallic acid biosynthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498579/
https://www.ncbi.nlm.nih.gov/pubmed/36140760
http://dx.doi.org/10.3390/genes13091592
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