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Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex

Three strains of the Aspergillus versicolor complex were isolated from a salty marsh at a former uranium mining site in Thuringia, Germany. The strains from a metal-rich environment were not only highly salt tolerant (up to 20% NaCl), but at the same time could sustain elevated Cs and Sr (both up to...

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Autores principales: Harpke, Marie, Pietschmann, Sebastian, Ueberschaar, Nico, Krüger, Thomas, Kniemeyer, Olaf, Brakhage, Axel A., Nietzsche, Sandor, Kothe, Erika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498632/
https://www.ncbi.nlm.nih.gov/pubmed/36140799
http://dx.doi.org/10.3390/genes13091631
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author Harpke, Marie
Pietschmann, Sebastian
Ueberschaar, Nico
Krüger, Thomas
Kniemeyer, Olaf
Brakhage, Axel A.
Nietzsche, Sandor
Kothe, Erika
author_facet Harpke, Marie
Pietschmann, Sebastian
Ueberschaar, Nico
Krüger, Thomas
Kniemeyer, Olaf
Brakhage, Axel A.
Nietzsche, Sandor
Kothe, Erika
author_sort Harpke, Marie
collection PubMed
description Three strains of the Aspergillus versicolor complex were isolated from a salty marsh at a former uranium mining site in Thuringia, Germany. The strains from a metal-rich environment were not only highly salt tolerant (up to 20% NaCl), but at the same time could sustain elevated Cs and Sr (both up to 100 mM) concentrations as well as other (heavy) metals present in the environment. During growth experiments when screening for differential cell morphology, the occurrence of guttation droplets was observed, specifically when elevated Sr concentrations of 25 mM were present in the media. To analyze the potential of metal tolerance being promoted by these excretions, proteomics and metabolomics of guttation droplets were performed. Indeed, proteins involved in up-regulated metabolic activities as well as in stress responses were identified. The metabolome verified the presence of amino sugars, glucose homeostasis-regulating substances, abscisic acid and bioactive alkaloids, flavones and quinones.
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spelling pubmed-94986322022-09-23 Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex Harpke, Marie Pietschmann, Sebastian Ueberschaar, Nico Krüger, Thomas Kniemeyer, Olaf Brakhage, Axel A. Nietzsche, Sandor Kothe, Erika Genes (Basel) Article Three strains of the Aspergillus versicolor complex were isolated from a salty marsh at a former uranium mining site in Thuringia, Germany. The strains from a metal-rich environment were not only highly salt tolerant (up to 20% NaCl), but at the same time could sustain elevated Cs and Sr (both up to 100 mM) concentrations as well as other (heavy) metals present in the environment. During growth experiments when screening for differential cell morphology, the occurrence of guttation droplets was observed, specifically when elevated Sr concentrations of 25 mM were present in the media. To analyze the potential of metal tolerance being promoted by these excretions, proteomics and metabolomics of guttation droplets were performed. Indeed, proteins involved in up-regulated metabolic activities as well as in stress responses were identified. The metabolome verified the presence of amino sugars, glucose homeostasis-regulating substances, abscisic acid and bioactive alkaloids, flavones and quinones. MDPI 2022-09-11 /pmc/articles/PMC9498632/ /pubmed/36140799 http://dx.doi.org/10.3390/genes13091631 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Harpke, Marie
Pietschmann, Sebastian
Ueberschaar, Nico
Krüger, Thomas
Kniemeyer, Olaf
Brakhage, Axel A.
Nietzsche, Sandor
Kothe, Erika
Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex
title Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex
title_full Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex
title_fullStr Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex
title_full_unstemmed Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex
title_short Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex
title_sort salt and metal tolerance involves formation of guttation droplets in species of the aspergillus versicolor complex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498632/
https://www.ncbi.nlm.nih.gov/pubmed/36140799
http://dx.doi.org/10.3390/genes13091631
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