Cargando…

The Complete Mitochondrial Genome of Spirobolus bungii (Diplopoda, Spirobolidae): The First Sequence for the Genus Spirobolus

Millipedes (Diplopoda) comprise one of the most important groups of large soil arthropods in terrestrial ecosystems; however, their phylogenetic relationships are poorly understood. Herein, the mitochondrial genome (mitogenome) of Spirobolus bungii was sequenced and annotated, which was 14,879 bp in...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Hanmei, Fang, Yu, Cao, Guohua, Shen, Caiqin, Liu, Hongyi, Ruan, Honghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498733/
https://www.ncbi.nlm.nih.gov/pubmed/36140755
http://dx.doi.org/10.3390/genes13091587
_version_ 1784794833420812288
author Xu, Hanmei
Fang, Yu
Cao, Guohua
Shen, Caiqin
Liu, Hongyi
Ruan, Honghua
author_facet Xu, Hanmei
Fang, Yu
Cao, Guohua
Shen, Caiqin
Liu, Hongyi
Ruan, Honghua
author_sort Xu, Hanmei
collection PubMed
description Millipedes (Diplopoda) comprise one of the most important groups of large soil arthropods in terrestrial ecosystems; however, their phylogenetic relationships are poorly understood. Herein, the mitochondrial genome (mitogenome) of Spirobolus bungii was sequenced and annotated, which was 14,879 bp in size and included 37 typical mitochondrial genes (13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), and 22 transfer RNA genes (tRNAs)). Most of the 13 PCGs had ATN (AT/A/T/G) as the start codon except for COX1, which used CGA, and most PCGs ended with the T end codon. By comparing the gene arrangements of the mitogenomes among Diplopoda species, rearrangement occurred between and within orders. In contrast to Narceus annularus, the mitogenome genes of S. bungii had consistent orders but were transcribed in completely opposite directions, which was a novel finding in Spirobolidae. Moreover, the phylogenetic relationships within Diplopoda, which were based on the sequences of 13 PCGs, showed that S. bungii was clustered with N. annularus, followed by Abacion magmun. This indicated that there might be a close relationship between Callipodida and Spirobolida. These results could contribute to further studies on the genetics and evolutionary processes of S. bungii and other Diplopoda species.
format Online
Article
Text
id pubmed-9498733
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94987332022-09-23 The Complete Mitochondrial Genome of Spirobolus bungii (Diplopoda, Spirobolidae): The First Sequence for the Genus Spirobolus Xu, Hanmei Fang, Yu Cao, Guohua Shen, Caiqin Liu, Hongyi Ruan, Honghua Genes (Basel) Article Millipedes (Diplopoda) comprise one of the most important groups of large soil arthropods in terrestrial ecosystems; however, their phylogenetic relationships are poorly understood. Herein, the mitochondrial genome (mitogenome) of Spirobolus bungii was sequenced and annotated, which was 14,879 bp in size and included 37 typical mitochondrial genes (13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), and 22 transfer RNA genes (tRNAs)). Most of the 13 PCGs had ATN (AT/A/T/G) as the start codon except for COX1, which used CGA, and most PCGs ended with the T end codon. By comparing the gene arrangements of the mitogenomes among Diplopoda species, rearrangement occurred between and within orders. In contrast to Narceus annularus, the mitogenome genes of S. bungii had consistent orders but were transcribed in completely opposite directions, which was a novel finding in Spirobolidae. Moreover, the phylogenetic relationships within Diplopoda, which were based on the sequences of 13 PCGs, showed that S. bungii was clustered with N. annularus, followed by Abacion magmun. This indicated that there might be a close relationship between Callipodida and Spirobolida. These results could contribute to further studies on the genetics and evolutionary processes of S. bungii and other Diplopoda species. MDPI 2022-09-03 /pmc/articles/PMC9498733/ /pubmed/36140755 http://dx.doi.org/10.3390/genes13091587 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Hanmei
Fang, Yu
Cao, Guohua
Shen, Caiqin
Liu, Hongyi
Ruan, Honghua
The Complete Mitochondrial Genome of Spirobolus bungii (Diplopoda, Spirobolidae): The First Sequence for the Genus Spirobolus
title The Complete Mitochondrial Genome of Spirobolus bungii (Diplopoda, Spirobolidae): The First Sequence for the Genus Spirobolus
title_full The Complete Mitochondrial Genome of Spirobolus bungii (Diplopoda, Spirobolidae): The First Sequence for the Genus Spirobolus
title_fullStr The Complete Mitochondrial Genome of Spirobolus bungii (Diplopoda, Spirobolidae): The First Sequence for the Genus Spirobolus
title_full_unstemmed The Complete Mitochondrial Genome of Spirobolus bungii (Diplopoda, Spirobolidae): The First Sequence for the Genus Spirobolus
title_short The Complete Mitochondrial Genome of Spirobolus bungii (Diplopoda, Spirobolidae): The First Sequence for the Genus Spirobolus
title_sort complete mitochondrial genome of spirobolus bungii (diplopoda, spirobolidae): the first sequence for the genus spirobolus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498733/
https://www.ncbi.nlm.nih.gov/pubmed/36140755
http://dx.doi.org/10.3390/genes13091587
work_keys_str_mv AT xuhanmei thecompletemitochondrialgenomeofspirobolusbungiidiplopodaspirobolidaethefirstsequenceforthegenusspirobolus
AT fangyu thecompletemitochondrialgenomeofspirobolusbungiidiplopodaspirobolidaethefirstsequenceforthegenusspirobolus
AT caoguohua thecompletemitochondrialgenomeofspirobolusbungiidiplopodaspirobolidaethefirstsequenceforthegenusspirobolus
AT shencaiqin thecompletemitochondrialgenomeofspirobolusbungiidiplopodaspirobolidaethefirstsequenceforthegenusspirobolus
AT liuhongyi thecompletemitochondrialgenomeofspirobolusbungiidiplopodaspirobolidaethefirstsequenceforthegenusspirobolus
AT ruanhonghua thecompletemitochondrialgenomeofspirobolusbungiidiplopodaspirobolidaethefirstsequenceforthegenusspirobolus
AT xuhanmei completemitochondrialgenomeofspirobolusbungiidiplopodaspirobolidaethefirstsequenceforthegenusspirobolus
AT fangyu completemitochondrialgenomeofspirobolusbungiidiplopodaspirobolidaethefirstsequenceforthegenusspirobolus
AT caoguohua completemitochondrialgenomeofspirobolusbungiidiplopodaspirobolidaethefirstsequenceforthegenusspirobolus
AT shencaiqin completemitochondrialgenomeofspirobolusbungiidiplopodaspirobolidaethefirstsequenceforthegenusspirobolus
AT liuhongyi completemitochondrialgenomeofspirobolusbungiidiplopodaspirobolidaethefirstsequenceforthegenusspirobolus
AT ruanhonghua completemitochondrialgenomeofspirobolusbungiidiplopodaspirobolidaethefirstsequenceforthegenusspirobolus