Cargando…
An Integrated Analysis of Lactation-Related miRNA and mRNA Expression Profiles in Donkey Mammary Glands
Donkey milk is consumed by humans for its nutritional and therapeutic properties. MicroRNAs (miRNAs) and messenger RNAs (mRNAs) have been implicated in the regulation of milk component synthesis and mammary gland development. However, the regulatory profile of the miRNAs and mRNAs involved in lactat...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498803/ https://www.ncbi.nlm.nih.gov/pubmed/36140805 http://dx.doi.org/10.3390/genes13091637 |
_version_ | 1784794850858631168 |
---|---|
author | Fei, Yaqi Gai, Yedan Liao, Qingchao Zhang, Linxi Li, Zheng Li, Bojiang Bai, Man Li, Na Deng, Liang |
author_facet | Fei, Yaqi Gai, Yedan Liao, Qingchao Zhang, Linxi Li, Zheng Li, Bojiang Bai, Man Li, Na Deng, Liang |
author_sort | Fei, Yaqi |
collection | PubMed |
description | Donkey milk is consumed by humans for its nutritional and therapeutic properties. MicroRNAs (miRNAs) and messenger RNAs (mRNAs) have been implicated in the regulation of milk component synthesis and mammary gland development. However, the regulatory profile of the miRNAs and mRNAs involved in lactation in donkeys is unclear. We performed mRNA-seq and miRNA-seq and constructed coexpression regulatory networks for the mammary glands during the lactating and nonlactating period of jennies. We identified 3144 differentially expressed (DE) mRNAs (987 upregulated mRNAs and 2157 downregulated mRNAs) and 293 DE miRNAs (231 upregulated miRNAs and 62 downregulated miRNAs) in the lactating group compared to the nonlactating group. The DE miRNA target mRNA were significantly associated with pathways related to RNA polymerase, glycosphingolipid biosynthesis, mRNA surveillance, ribosome biogenesis in eukaryotes, glycerophospholipid metabolism, Ras signaling, and the fly hippo signaling pathway. The mRNA–miRNA coregulation analysis showed that novel-m0032-3p, miR-195, miR-26-5p, miR-23-3p, miR-674-3p, and miR-874-3p are key miRNAs that target mRNAs involved in immunity and milk lipid, protein, and vitamin metabolism in the jenny mammary gland. Our results improve the current knowledge of the molecular mechanisms regulating bioactive milk component metabolism in the mammary glands and could be used to improve milk production in donkeys. |
format | Online Article Text |
id | pubmed-9498803 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94988032022-09-23 An Integrated Analysis of Lactation-Related miRNA and mRNA Expression Profiles in Donkey Mammary Glands Fei, Yaqi Gai, Yedan Liao, Qingchao Zhang, Linxi Li, Zheng Li, Bojiang Bai, Man Li, Na Deng, Liang Genes (Basel) Article Donkey milk is consumed by humans for its nutritional and therapeutic properties. MicroRNAs (miRNAs) and messenger RNAs (mRNAs) have been implicated in the regulation of milk component synthesis and mammary gland development. However, the regulatory profile of the miRNAs and mRNAs involved in lactation in donkeys is unclear. We performed mRNA-seq and miRNA-seq and constructed coexpression regulatory networks for the mammary glands during the lactating and nonlactating period of jennies. We identified 3144 differentially expressed (DE) mRNAs (987 upregulated mRNAs and 2157 downregulated mRNAs) and 293 DE miRNAs (231 upregulated miRNAs and 62 downregulated miRNAs) in the lactating group compared to the nonlactating group. The DE miRNA target mRNA were significantly associated with pathways related to RNA polymerase, glycosphingolipid biosynthesis, mRNA surveillance, ribosome biogenesis in eukaryotes, glycerophospholipid metabolism, Ras signaling, and the fly hippo signaling pathway. The mRNA–miRNA coregulation analysis showed that novel-m0032-3p, miR-195, miR-26-5p, miR-23-3p, miR-674-3p, and miR-874-3p are key miRNAs that target mRNAs involved in immunity and milk lipid, protein, and vitamin metabolism in the jenny mammary gland. Our results improve the current knowledge of the molecular mechanisms regulating bioactive milk component metabolism in the mammary glands and could be used to improve milk production in donkeys. MDPI 2022-09-12 /pmc/articles/PMC9498803/ /pubmed/36140805 http://dx.doi.org/10.3390/genes13091637 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fei, Yaqi Gai, Yedan Liao, Qingchao Zhang, Linxi Li, Zheng Li, Bojiang Bai, Man Li, Na Deng, Liang An Integrated Analysis of Lactation-Related miRNA and mRNA Expression Profiles in Donkey Mammary Glands |
title | An Integrated Analysis of Lactation-Related miRNA and mRNA Expression Profiles in Donkey Mammary Glands |
title_full | An Integrated Analysis of Lactation-Related miRNA and mRNA Expression Profiles in Donkey Mammary Glands |
title_fullStr | An Integrated Analysis of Lactation-Related miRNA and mRNA Expression Profiles in Donkey Mammary Glands |
title_full_unstemmed | An Integrated Analysis of Lactation-Related miRNA and mRNA Expression Profiles in Donkey Mammary Glands |
title_short | An Integrated Analysis of Lactation-Related miRNA and mRNA Expression Profiles in Donkey Mammary Glands |
title_sort | integrated analysis of lactation-related mirna and mrna expression profiles in donkey mammary glands |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498803/ https://www.ncbi.nlm.nih.gov/pubmed/36140805 http://dx.doi.org/10.3390/genes13091637 |
work_keys_str_mv | AT feiyaqi anintegratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT gaiyedan anintegratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT liaoqingchao anintegratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT zhanglinxi anintegratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT lizheng anintegratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT libojiang anintegratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT baiman anintegratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT lina anintegratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT dengliang anintegratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT feiyaqi integratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT gaiyedan integratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT liaoqingchao integratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT zhanglinxi integratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT lizheng integratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT libojiang integratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT baiman integratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT lina integratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands AT dengliang integratedanalysisoflactationrelatedmirnaandmrnaexpressionprofilesindonkeymammaryglands |