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An ACE2-dependent Sarbecovirus in Russian bats is resistant to SARS-CoV-2 vaccines

Spillover of sarbecoviruses from animals to humans has resulted in outbreaks of severe acute respiratory syndrome SARS-CoVs and the ongoing COVID-19 pandemic. Efforts to identify the origins of SARS-CoV-1 and -2 has resulted in the discovery of numerous animal sarbecoviruses–the majority of which ar...

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Autores principales: Seifert, Stephanie N., Bai, Shuangyi, Fawcett, Stephen, Norton, Elizabeth B., Zwezdaryk, Kevin J., Robinson, James, Gunn, Bronwyn, Letko, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498966/
https://www.ncbi.nlm.nih.gov/pubmed/36136995
http://dx.doi.org/10.1371/journal.ppat.1010828
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author Seifert, Stephanie N.
Bai, Shuangyi
Fawcett, Stephen
Norton, Elizabeth B.
Zwezdaryk, Kevin J.
Robinson, James
Gunn, Bronwyn
Letko, Michael
author_facet Seifert, Stephanie N.
Bai, Shuangyi
Fawcett, Stephen
Norton, Elizabeth B.
Zwezdaryk, Kevin J.
Robinson, James
Gunn, Bronwyn
Letko, Michael
author_sort Seifert, Stephanie N.
collection PubMed
description Spillover of sarbecoviruses from animals to humans has resulted in outbreaks of severe acute respiratory syndrome SARS-CoVs and the ongoing COVID-19 pandemic. Efforts to identify the origins of SARS-CoV-1 and -2 has resulted in the discovery of numerous animal sarbecoviruses–the majority of which are only distantly related to known human pathogens and do not infect human cells. The receptor binding domain (RBD) on sarbecoviruses engages receptor molecules on the host cell and mediates cell invasion. Here, we tested the receptor tropism and serological cross reactivity for RBDs from two sarbecoviruses found in Russian horseshoe bats. While these two viruses are in a viral lineage distinct from SARS-CoV-1 and -2, the RBD from one virus, Khosta 2, was capable of using human ACE2 to facilitate cell entry. Viral pseudotypes with a recombinant, SARS-CoV-2 spike encoding for the Khosta 2 RBD were resistant to both SARS-CoV-2 monoclonal antibodies and serum from individuals vaccinated for SARS-CoV-2. Our findings further demonstrate that sarbecoviruses circulating in wildlife outside of Asia also pose a threat to global health and ongoing vaccine campaigns against SARS-CoV-2
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spelling pubmed-94989662022-09-23 An ACE2-dependent Sarbecovirus in Russian bats is resistant to SARS-CoV-2 vaccines Seifert, Stephanie N. Bai, Shuangyi Fawcett, Stephen Norton, Elizabeth B. Zwezdaryk, Kevin J. Robinson, James Gunn, Bronwyn Letko, Michael PLoS Pathog Research Article Spillover of sarbecoviruses from animals to humans has resulted in outbreaks of severe acute respiratory syndrome SARS-CoVs and the ongoing COVID-19 pandemic. Efforts to identify the origins of SARS-CoV-1 and -2 has resulted in the discovery of numerous animal sarbecoviruses–the majority of which are only distantly related to known human pathogens and do not infect human cells. The receptor binding domain (RBD) on sarbecoviruses engages receptor molecules on the host cell and mediates cell invasion. Here, we tested the receptor tropism and serological cross reactivity for RBDs from two sarbecoviruses found in Russian horseshoe bats. While these two viruses are in a viral lineage distinct from SARS-CoV-1 and -2, the RBD from one virus, Khosta 2, was capable of using human ACE2 to facilitate cell entry. Viral pseudotypes with a recombinant, SARS-CoV-2 spike encoding for the Khosta 2 RBD were resistant to both SARS-CoV-2 monoclonal antibodies and serum from individuals vaccinated for SARS-CoV-2. Our findings further demonstrate that sarbecoviruses circulating in wildlife outside of Asia also pose a threat to global health and ongoing vaccine campaigns against SARS-CoV-2 Public Library of Science 2022-09-22 /pmc/articles/PMC9498966/ /pubmed/36136995 http://dx.doi.org/10.1371/journal.ppat.1010828 Text en © 2022 Seifert et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Seifert, Stephanie N.
Bai, Shuangyi
Fawcett, Stephen
Norton, Elizabeth B.
Zwezdaryk, Kevin J.
Robinson, James
Gunn, Bronwyn
Letko, Michael
An ACE2-dependent Sarbecovirus in Russian bats is resistant to SARS-CoV-2 vaccines
title An ACE2-dependent Sarbecovirus in Russian bats is resistant to SARS-CoV-2 vaccines
title_full An ACE2-dependent Sarbecovirus in Russian bats is resistant to SARS-CoV-2 vaccines
title_fullStr An ACE2-dependent Sarbecovirus in Russian bats is resistant to SARS-CoV-2 vaccines
title_full_unstemmed An ACE2-dependent Sarbecovirus in Russian bats is resistant to SARS-CoV-2 vaccines
title_short An ACE2-dependent Sarbecovirus in Russian bats is resistant to SARS-CoV-2 vaccines
title_sort ace2-dependent sarbecovirus in russian bats is resistant to sars-cov-2 vaccines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498966/
https://www.ncbi.nlm.nih.gov/pubmed/36136995
http://dx.doi.org/10.1371/journal.ppat.1010828
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