Genome Editing of Golden SNP-Carrying Lycopene Epsilon-Cyclase (LcyE) Gene Using the CRSPR-Cas9/HDR and Geminiviral Replicon System in Rice

Lycopene epsilon-cyclase (LcyE) is a key enzyme in the carotenoid biosynthetic pathway of higher plants. Using the CRSPR/Cas9 and the geminiviral replicon, we optimized a method for targeted mutagenesis and golden SNP replacement of the LcyE gene in rice. We have exploited the geminiviral replicon a...

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Detalles Bibliográficos
Autores principales: Kim, Jong Hee, Yu, Jihyeon, Kim, Hee Kyoung, Kim, Jin Young, Kim, Me-Sun, Cho, Yong-Gu, Bae, Sangsu, Kang, Kwon Kyoo, Jung, Yu Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499184/
https://www.ncbi.nlm.nih.gov/pubmed/36142294
http://dx.doi.org/10.3390/ijms231810383

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499184/
https://www.ncbi.nlm.nih.gov/pubmed/36142294
http://dx.doi.org/10.3390/ijms231810383

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